Patents by Inventor Ellen Guthrie

Ellen Guthrie has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Deglycosylation reagents and methods
    Patent number: 9964548
    Abstract: Compositions and methods are provided for efficiently preparing a completely deglycosylated antibody where efficiency is measured in relative amounts of reagents in soluble or lyophilized form, and time and temperature of the reaction. Compositions and methods are also provided for separating substantially all N-linked glycans from a glycosylated antibody and for preserving functionality of the antibody. The methods are compatible with glycan labeling and protease digestion without the need for prior purification steps.
    Type: Grant
    Filed: December 28, 2016
    Date of Patent: May 8, 2018
    Assignee: New England Biolabs, Inc.
    Inventors: Paula Magnelli, Ellen Guthrie, Christopher H. Taron, Ming-Qun Xu, John Buswell
  • Deglycosylation Reagents and Methods
    Publication number: 20170138959
    Abstract: Compositions and methods are provided for efficiently preparing a completely deglycosylated antibody where efficiency is measured in relative amounts of reagents in soluble or lyophilized form, and time and temperature of the reaction. Compositions and methods are also provided for separating substantially all N-linked glycans from a glycosylated antibody and for preserving functionality of the antibody. The methods are compatible with glycan labeling and protease digestion without the need for prior purification steps.
    Type: Application
    Filed: December 28, 2016
    Publication date: May 18, 2017
    Applicant: New England Biolabs, Inc.
    Inventors: Paula Magnelli, Ellen Guthrie, Christopher H. Taron, Ming-Qun Xu, John Buswell
  • Deglycosylation Reagents and Methods
    Publication number: 20150346194
    Abstract: Compositions and methods are provided for efficiently preparing a completely deglycosylated antibody where efficiency is measured in relative amounts of reagents in soluble or lyophilized form, and time and temperature of the reaction. Compositions and methods are also provided for separating substantially all N-linked glycans from a glycosylated antibody and for preserving functionality of the antibody. The methods are compatible with glycan labeling and protease digestion without the need for prior purification steps.
    Type: Application
    Filed: May 29, 2015
    Publication date: December 3, 2015
    Applicant: NEW ENGLAND BIOLABS, INC.
    Inventors: Paula Magnelli, Ellen Guthrie, Christopher H. Taron, Ming-Qun Xu, John Buswell
  • Endoglycosidases that cleave -linked glycans
    Patent number: 8969034
    Abstract: Methods and compositions have been described that relate to a newly identified polypeptide family wherein each member has O-glycosidase activity and specified sequence characteristics. This family of enzymes can be used for example for cleaving O-linked glycans and for synthesis of neoglycopeptides or neoglycoproteins.
    Type: Grant
    Filed: April 7, 2009
    Date of Patent: March 3, 2015
    Assignee: New England Biolabs, Inc.
    Inventors: Dimitris Koutsioulis, Ellen Guthrie
  • Endoglycosidases that Cleave O-linked Glycans
    Publication number: 20110053215
    Abstract: Methods and compositions have been described that relate to a newly identified polypeptide family wherein each member has O-glycosidase activity and specified sequence characteristics. This family of enzymes can be used for example for cleaving O-linked glycans and for synthesis of neoglycopeptides or neoglycoproteins.
    Type: Application
    Filed: April 7, 2009
    Publication date: March 3, 2011
    Applicant: NEW ENGLAND BIOLABS, INC.
    Inventors: Dimitris Koutsioulis, Ellen Guthrie
  • Overexpression, purification and characterization of a thermolabile phosphatase
    Patent number: 7319014
    Abstract: Compositions for an alkaline phosphatase and methods for over-expression and purification of thermolabile Antarctic phosphatase (TAP) are provided. Uses for TAP include dephosphorylation of nucleic acids, sugars, peptides and proteins. TAP as described herein has advantages over phosphatases from other sources with respect to thermolability at 65° C. and efficiency of dephosphorylation activity at approximately neutral pH.
    Type: Grant
    Filed: February 20, 2004
    Date of Patent: January 15, 2008
    Assignee: New England Biolabs, Inc.
    Inventors: Ellen Guthrie, Theodore Davis, Jack Benner, II
  • Overexpression, purification and characterization of a thermoolabile phosphatase
    Publication number: 20060252118
    Abstract: Compositions for an alkaline phosphatase and methods for over-expression and purification of thermolabile Antarctic phosphatase (TAP) are provided. Uses for TAP include dephosphorylation of nucleic acids, sugars, peptides and proteins. TAP as described herein has advantages over phosphatases from other sources with respect to thermolability at 65° C. and efficiency of dephosphorylation activity at approximately neutral pH.
    Type: Application
    Filed: February 20, 2004
    Publication date: November 9, 2006
    Inventors: Ellen Guthrie, Theodore Davis, Jack Benner