Abstract: The present invention is directed to methods and compositions for detection of target analytes, comprising proteins and nucleic acids, in multiple cellular compartments. Preferred embodiments comprise the use of complement-mediated assays. Methods and compositions for monitoring multiple stages of disease and infection are presented.

Abstract: The present invention comprises methods and compositions using biological factors, such as complement components, and manipulation of cells of erythroblastic lineage and myeloid lineage to facilitate clearance of pathologic targets from the blood stream in specific phagocytic compartment.

Abstract: A method of detecting a target analyte comprising the steps of providing a sample suspected of having a target analyte, protecting a specific target analyte, eliminating non-specific analytes, and detecting the presence of target with a signal.

Abstract: The present invention provides methods and compositions using biological factors, such as complement components, and manipulation of cells of erythroblastic lineage and myeloid lineage to facilitate clearance of pathologic targets from the blood stream of a patient in specific phagocytic compartment.

Abstract: The present invention is directed to methods and compositions for detection of target analytes, comprising proteins and nucleic acids, in multiple cellular compartments. Preferred embodiments comprise the use of complement-mediated assays. Methods and compositions for monitoring multiple stages of disease and infection are presented.

Abstract: The present invention is directed to methods and compositions for signal amplification used in analyzing large quantities of a DNA, protein, cellular or RNA sample to detect a target analyte. The methods of the present invention, Zymogen Mediated Signal Amplification, ZMSA, minimize the occurrence of false positive and false negative conclusions in diagnostic results without DNA amplification or reverse transcriptase reactions. The signal amplification methods of the present invention employ highly increased signal production capabilities of an enzyme and its substrate. Embodiments of the present invention may take the form of an autocatalytic reaction or a synthetic enzyme.

Abstract: Methods and compositions are provided for the detection of specific nucleic acid sequences purified from cellular or tissue sources. More particularly, the present invention includes methods and compositions for the detection of nucleic acid sequences using a protection molecule that forms a protected nucleic acid sequence (PNAS) such as a triplex or duplex nucleic acid structure that includes the target nucleic acid sequence. An assay using the methods of the present invention may include one, two or three levels of specificity to minimize false positive signals. An assay using the methods or compositions of the present invention can be performed on large amounts of purified DNA in a single test, with high levels of sensitivity, thus eliminating the need for DNA amplification procedures.

Abstract: Methods and compositions are provided for the detection of specific nucleic acid sequences purified from cellular or tissue sources. More particularly, the present invention includes methods and compositions for the detection of nucleic acid sequences using a protection molecule that forms a protected nucleic acid sequence (PNAS) such as a triplex or duplex nucleic acid structure that includes the target nucleic acid sequence. An assay using the methods of the present invention may include one, two or three levels of specificity to minimize false positive signals. An assay using the methods or compositions of the present invention can be performed on large amounts of purified DNA in a single test, with high levels of sensitivity, thus eliminating the need for DNA amplification procedures.

Abstract: Methods and compositions are provided for the detection of specific nucleic acid sequences purified from cellular or tissue sources. More particularly, the present invention includes methods and compositions for the detection of nucleic acid sequences using a protection molecule that forms a protected nucleic acid sequence (PNAS) such as a triplex or duplex nucleic acid structure that includes the target nucleic acid sequence. An assay using the methods of the present invention may include one, two or three levels of specificity to minimize false positive signals. An assay using the methods or compositions of the present invention can be performed on large amounts of purified DNA in a single test, with high levels of sensitivity, thus eliminating the need for DNA amplification procedures.

Abstract: Methods and compositions are provided for the detection of specific nucleic acid sequences purified from cellular or tissue sources. More particularly, the present invention includes methods and compositions for the detection of nucleic acid sequences using a protection molecule that forms a protected nucleic acid sequence (PNAS) such as a triplex or duplex nucleic acid structure that includes the target nucleic acid sequence. An assay using the methods of the present invention may include one, two or three levels of specificity to minimize false positive signals. An assay using the methods or compositions of the present invention can be performed on large amounts of purified DNA in a single test, with high levels of sensitivity, thus eliminating the need for DNA amplification procedures.