Patents by Inventor Eloy Bernal Mendez
Eloy Bernal Mendez has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 8663923Abstract: A nucleotide probe permitting the detection of nucleic acids and constituted of a labeled nucleotide strand having three fragments: a first fragment having a first closing sequence, a second fragment, all or part of which has a recognition sequence for the molecular recognition of the target nucleic acid and a third fragment having a second closing sequence, and at least two markers, one of the ends of the strand of the detection probe being free of any marker, in which, when the two closing sequences are hybridized together, the detection probe has a completely circular shape, the closing sequences thus maintaining the probe in a conformation that cannot be detected in the absence of the target nucleic acid.Type: GrantFiled: July 3, 2009Date of Patent: March 4, 2014Assignee: bioMerieuxInventors: Ali Laayoun, Eloy Bernal Mendez
-
Patent number: 8637322Abstract: The present invention relates to a method for labeling biological molecules of interest contained in a biological sample, consisting in: a) providing a reaction vessel, b) immobilizing capture molecules, which are capable of binding a label of the biological molecules of interest, on a solid support of the vessel, c) introducing the biological sample but also at least one label of the biological molecules of interest into said vessel and optionally any ingredient required for labeling or prelabeling the molecules of interest, d) incubating the vessel content and immobilizing the label which is not reacted with the molecules of interest by binding to the capture molecules, and e) using the labeled molecules of interest for subsequent steps. A method for treating a biological sample is also disclosed. Said invention is preferably used in a manual or automated method for purifying nucleic acids.Type: GrantFiled: May 31, 2006Date of Patent: January 28, 2014Assignee: BiomerieuxInventors: Eloy Bernal-Mendez, Ali Laayoun, Lionel Menou
-
Patent number: 8158345Abstract: The present invention relates to a labeled oligonucleotide comprising a first nucleotide segment and a second nucleotide segment, complementary to a target sequence, a fluorophore, a quencher and at least one alpha-anomeric nucleoside. The invention also relates to the use of such an oligonucleotide and also to a process using such an oligonucleotide.Type: GrantFiled: September 26, 2007Date of Patent: April 17, 2012Assignee: BiomerieuxInventors: Eloy Bernal-Mendez, Ali Laayoun, Alain Laurent
-
Publication number: 20110104762Abstract: The present invention relates to a detection probe acting by molecular recognition of a target sequence, comprising successively in the 5?-3? direction: a first nucleotide segment comprising a sterically hindering structure at its 5? end, and a second nucleotide segment, complementary to the target sequence.Type: ApplicationFiled: December 20, 2010Publication date: May 5, 2011Applicant: BIOMERIEUXInventors: Eloy Bernal-Mendez, Ali Laayoun, Alain Laurent
-
Publication number: 20110091898Abstract: A nucleotide probe permitting the detection of nucleic acids and constituted of a labeled nucleotide strand having three fragments: a first fragment having a first closing sequence, a second fragment, all or part of which has a recognition sequence for the molecular recognition of the target nucleic acid and a third fragment having a second closing sequence, and at least two markers, one of the ends of the strand of the detection probe being free of any marker, in which, when the two closing sequences are hybridized together, the detection probe has a completely circular shape, the closing sequences thus maintaining the probe in a conformation that cannot be detected in the absence of the target nucleic acid.Type: ApplicationFiled: July 3, 2009Publication date: April 21, 2011Applicant: BIOMERIEUXInventors: Ali Laayoun, Eloy Bernal Mendez
-
Patent number: 7691635Abstract: The present invention relates to a temperature-stable labeling reagent of formula (0): in which: R1 represents H or an alkyl, aryl or substituted aryl group, R2 represents a detectable marker or at least two detectable markers interlinked by at least one multimeric structure, L is a linker arm comprising a linear chain of at least two covalent bonds and n is an integer equal to 0 or 1, R3 and R4 represent, independently of one another: H, NO2, Cl, Br, F, I, R2-(L)n-Y—X—, OR, SR, NR2, R, NHCOR, CONHR, COOR, —CO—NH—(CH2)3—(O—CH2—CH2)3—CH2—NH—R2, —CO—NH—(CH2)3—(O—CH2—CH2)4—CH2—NH—R2 with R=alkyl or aryl, A is a linker arm comprising at least one covalent double bond enabling the conjugation of the diazo function with the aromatic ring and u is an integer between 0 and 2, preferably 0 or 1, —Y—X— represents —CONH—, —NHCO—, —CH2O—, —CH2S—, —Z— represents —NH—, —NHCO—, —CONH— or —O—, m is an integer between 1 and 10, preferably between 1 and 3, and p is an integer between 1 and 10, preferably between 1 and 3Type: GrantFiled: March 24, 2005Date of Patent: April 6, 2010Assignee: BiomerieuxInventors: Ali Laayoun, Eloy Bernal-Mendez
-
Publication number: 20090311688Abstract: The present invention relates to a labeled oligonucleotide comprising a first nucleotide segment and a second nucleotide segment, complementary to a target sequence, characterized in that it comprises a fluorophore, a quencher and at least one alpha-anomeric nucleoside. The invention also relates to the use of such an oligonucleotide and also to a process using such an oligonucleotide.Type: ApplicationFiled: September 26, 2007Publication date: December 17, 2009Applicant: BIOMERIEUXInventors: Eloy Bernal-Mendez, Ali Laayoun, Alain Laurent
-
Publication number: 20090215188Abstract: The present invention relates to a method for labeling biological molecules of interest contained in a biological sample, consisting in: a) providing a reaction vessel, b) immobilizing capture molecules, which are capable of binding a label of the biological molecules of interest, on a solid support of the vessel, c) introducing the biological sample but also at least one label of the biological molecules of interest into said vessel and optionally any ingredient required for labeling or prelabeling the molecules of interest, d) incubating the vessel content and immobilizing the label which is not reacted with the molecules of interest by binding to the capture molecules, and e) using the labeled molecules of interest for subsequent steps. A method for treating a biological sample is also disclosed. Said invention is preferably used in a manual or automated method for purifying nucleic acids.Type: ApplicationFiled: May 31, 2006Publication date: August 27, 2009Applicant: BIOMERIEUXInventors: Eloy Bernal-Mendez, Ali Laayoun, Lionel Menou
-
Publication number: 20090117575Abstract: The present invention relates to a detection probe acting by molecular recognition of a target sequence, comprising successively in the 5?-3? direction: a first nucleotide segment comprising a sterically hindering structure at its 5? end, and a second nucleotide segment, complementary to the target sequence.Type: ApplicationFiled: April 23, 2007Publication date: May 7, 2009Applicant: BIOMERIEUXInventors: Eloy Bernal-Mendez, Ali Laayoun, Alain Laurent
-
Patent number: 7338805Abstract: The present invention relates to a temperature-stable labeling reagent of formula: in which R1 represents H or an alkyl, aryl or substituted aryl group, R2 represents a detectable marker or at least two detectable markers linked together by at least one multimeric structure, L is a linking arm containing a linear succession of at least two covalent bonds and n an integer equal to 0 or 1, R3 and R4 represent independently of each other: H, NO2, Cl, Br, F, I, R2-(L)n-Y—X—, OR, SR, NR2, R, NHCOR, CONHR, COOR with R=alkyl or aryl, A is a linking arm containing at least one covalent double bond allowing conjugation of the diazo functional group with the aromatic ring and u is an integer between 0 and 2, preferably equal to 0 or 1, and —Y—X— represents —CONH—, —NHCO—, —CH2O—, —CH2S—.Type: GrantFiled: May 3, 2002Date of Patent: March 4, 2008Assignees: Bio Merieux, Universite Joseph Fourier Domaine, Centre National de la Recherche ScientifiqueInventors: Cecile Bourget, Jean Lhomme, Ali Laayoun, Mitsuharu Kotera, Emmanuelle Trevisiol, Lionel Menou, Eloy Bernal Mendez
-
Publication number: 20080032288Abstract: The present invention relates to a temperature-stable labeling reagent of formula (0): in which: R1 represents H or an alkyl, aryl or substituted aryl group, R2 represents a detectable marker or at least two detectable markers interlinked by at least one multimeric structure, L is a linker arm comprising a linear chain of at least two covalent bonds and n is an integer equal to 0 or 1, R3 and R4 represent, independently of one another: H, NO2, Cl, Br, F, I, R2 —(L)n—Y—X—, OR, SR, NR2, R, NHCOR, CONHR, COOR, —CO—NH—(CH2)3—(O—CH2—CH2)3-CH2—NH—R2, —CO—NH—(CH2)3—(O—CH2—CH2)4—CH2—NH—R2 with R=alkyl or aryl, A is a linker arm comprising at least one covalent double bond enabling the conjugation of the diazo function with the aromatic ring and u is an integer between 0 and 2, preferably 0 or 1, —Y—X— represents —CONH—, —NHCO—, —CH2O—, —CH2S—, -Z-represents —NH—, —NHCO—, —CONH— or —O—, m is an integer between 1 and 10, preferably between 1 and 3, and p is an integer between 1 and 10, preferably between 1 and 3Type: ApplicationFiled: March 24, 2005Publication date: February 7, 2008Applicant: bioMerieuxInventors: Ali Laayoun, Eloy Bernal-Mendez
-
Publication number: 20030186448Abstract: The present invention relates to a temperature-stable labeling reagent of formula: 1Type: ApplicationFiled: May 3, 2002Publication date: October 2, 2003Applicant: BIO MERIEUXInventors: Cecile Bourget, Jean Lhomme, Ali Laayoun, Mitsuharu Kotera, Emmanuelle Trevisiol, Lionel Menou, Eloy Bernal Mendez