Abstract: Compositions comprising an inactive cellulosome-producing microorganism and detectable extra-cellular osidase are provided. Compositions comprising an inactive Caldicellulosiruptor bescii and detectable extra-cellular beta-glucosidase or extra-cellular beta-xylosidase are also provided.

Abstract: Compositions comprising unprocessed cell pellets of a cellulosome-producing microorganism grown on cellulosic biomass are provided. Further provided are methods for producing the compositions and uses thereof in hydrolysis of cellulosic substrates. In particular, the compositions advantageously contain extracellular beta-glucosidase, either expressed on the cells themselves or extrinsically added to the cell pellets.

Abstract: Compositions comprising unprocessed cell pellets of a cellulosome-producing microorganism grown on cellulosic biomass are provided. Further provided are methods for producing the compositions and uses thereof in hydrolysis of cellulosic substrates. In particular, the compositions advantageously contain extracellular beta-glucosidase, either expressed on the cells themselves or extrinsically added to the cell pellets.

Abstract: The present invention provides compositions comprising a cellulolytic bacterium and an isolated BETA-glucosidase enzyme or comprising cellulolytic bacterium that expresses a secreted recombinant BETA-glucosidase enzyme, and methods of using same for hydrolysis of cellulose and cellulosic feedstocks.

Abstract: The present invention relates to libraries of chimeric proteins and libraries of polynucleotides encoding same, wherein each chimeric protein comprises a cellulose-binding region and at least one exogenous moiety, the exogenous moiety preferably comprising at least one peptide. The present invention further relates to use of the libraries for screening assays. Methods of high throughput screening using these libraries for biological active moieties such as enzymes and antibodies are also included within the scope of the present invention.

Abstract: The present invention provides microarrays of proteins comprising a cellulose binding region as a means for binding to a cellulase substrate such as cellulose. The cellulose binding region may further contain biologically active exogenous moities introduced therein while maintaining the ability to bind cellulase substrates with high affinity. In addition, methods of construction of the microarrays and using the microarrays are disclosed. The microarrays of the invention are particularly useful for displaying peptide libraries, random or rationally designed, and for high throughput screening for ligands, epitopes or ligand binding sites.

Abstract: AbstractThe present invention relates to a multi-functional targeting complex for inducing a specific immuno-stimulatory reaction, preferably, phagocytosis, against at least one target pathogenic agent. The complex of the invention comprises at least one target recognition component comprising a molecule that specifically binds to the desired target agent, an immuno-active component comprising an immuno-stimulatory agent; and optionally, a connecting component that associates the targeting component and the immuno-active component. The complex of the invention provides an effective therapeutic prevention and treatment of various pathogenic disorders, such as mastitis in caws and furunculosis in fish. The invention further relates to compositions comprising the targeting complex, methods of treatment and uses thereof.

Abstract: Biotin-binding modified avidin-type molecules are provided in which the essential tyrosine residue in the biotin-binding site is modified in such a way that its pKa is decreased in comparison to the pKa of the unmodified tyrosine residue in the corresponding unmodified avidin-type molecule. The avidin-type molecules include: (i) native egg-white avidin; (ii) recombinant avidin; (iii) deglycosylated forms of avidin; (iv) bacterial streptavidin; (v) recombinant streptavidin; (vi) truncated streptavidin; and (vii) derivatives of (i)-(vi) which are modified at sites other than the essential tyrosine residue. The modification is achieved by substitution at one or both ortho positions to the hydroxy radical of the tyrosine residue by radicals such as nitro, halogen, azo and amino. The modified avidin-type molecules can be used in all applications of the avidin-biotin technology.