Patents by Inventor Elysia K. Krieger
Elysia K. Krieger has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230383301Abstract: The present disclosure is in the field of plant transformation. The disclosure provides methods for increasing the rate of site-directed integration of a sequence of interest in plants. The disclosure also provides methods for increasing the efficiency of Rhizobiales-mediated plant transformation.Type: ApplicationFiled: June 6, 2023Publication date: November 30, 2023Inventors: Elysia K. KRIEGER, Richard J. LAWRENCE, Zarir E. VAGHCHHIPAWALA, Jianping XU, Xudong YE
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Patent number: 11732268Abstract: The present disclosure is in the field of plant transformation. The disclosure provides methods for increasing the rate of site-directed integration of a sequence of interest in plants. The disclosure also provides methods for increasing the efficiency of Rhizobiales-mediated plant transformation.Type: GrantFiled: June 27, 2017Date of Patent: August 22, 2023Assignee: Monsanto Technology LLCInventors: Elysia K. Krieger, Richard J. Lawrence, Zarir E. Vaghchhipawala, Jianping Xu, Xudong Ye
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Publication number: 20220195405Abstract: The present disclosure provides compositions related to engineered CRISPR endonuclease proteins that have a reduced ability to non-specifically cleave single-stranded DNA (ssDNA) as compared to its reference wildtype protein. This disclosure also provides methods related to the use of, and generation of, engineered CRISPR endonuclease proteins that have a reduced ability to non-specifically cleave ssDNA as compared to its reference wildtype protein.Type: ApplicationFiled: December 16, 2021Publication date: June 23, 2022Applicant: MONSANTO TECHNOLOGY LLCInventors: Graeme S. GARVEY, Elysia K. KRIEGER
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Patent number: 11008580Abstract: This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.Type: GrantFiled: April 20, 2018Date of Patent: May 18, 2021Assignee: MONSANTO TECHNOLOGY LLCInventors: Edwards M. Allen, Sara E. Heisel, Sergey Ivashuta, Elysia K. Krieger, Jennifer Lutke, Robert J. Meister, Yuanji Zhang
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Patent number: 10793869Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.Type: GrantFiled: June 29, 2017Date of Patent: October 6, 2020Assignee: MONSANTO TECHNOLOGY LLCInventors: Edwards M. Allen, Larry A Gilbertson, Sara E. Heisel, Shihshieh Huang, Elysia K. Krieger, Thomas M. Malvar
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Patent number: 10435686Abstract: This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.Type: GrantFiled: December 11, 2014Date of Patent: October 8, 2019Assignee: MONSANTO TECHNOLOGY LLCInventors: Edwards M. Allen, Barry S. Goldman, Liang Guo, Sara E. Heisel, Shihshieh Huang, Sergey I. Ivashuta, David K. Kovalic, Elysia K. Krieger, James K. Roberts, Yuanji Zhang
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Publication number: 20190211344Abstract: The present disclosure is in the field of plant transformation. The disclosure provides methods for increasing the rate of site-directed integration of a sequence of interest in plants. The disclosure also provides methods for increasing the efficiency of Rhizobiales-mediated plant transformation.Type: ApplicationFiled: June 27, 2017Publication date: July 11, 2019Inventors: Elysia K. KRIEGER, Richard J. LAWRENCE, Zarir E. VAGHCHHIPAWALA, Jianping XU, Xudong YE
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Publication number: 20180237791Abstract: This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.Type: ApplicationFiled: April 20, 2018Publication date: August 23, 2018Inventors: Edwards M. ALLEN, Sara E. HEISEL, Sergey IVASHUTA, Elysia K. KRIEGER, Jennifer LUTKE, Robert J. MEISTER, Yuanji ZHANG
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Patent number: 9976152Abstract: This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.Type: GrantFiled: March 9, 2016Date of Patent: May 22, 2018Assignee: MONSANTO TECHNOLOGY LLCInventors: Edwards M. Allen, Sara E. Heisel, Sergey Ivashuta, Elysia K. Krieger, Jennifer Lutke, Robert J. Meister, Yuanji Zhang
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Publication number: 20180002713Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.Type: ApplicationFiled: June 29, 2017Publication date: January 4, 2018Inventors: Edwards M. ALLEN, Larry A. GILBERTSON, Sara E. HEISEL, Shihshieh HUANG, Elysia K. KRIEGER, Thomas M. MALVAR
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Patent number: 9708620Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.Type: GrantFiled: December 24, 2014Date of Patent: July 18, 2017Assignee: Monsanto Technology LLCInventors: Edwards M. Allen, Larry A. Gilbertson, Sara E. Heisel, Shihshieh Huang, Elysia K. Krieger, Thomas M. Malvar
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Publication number: 20160186194Abstract: This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.Type: ApplicationFiled: March 9, 2016Publication date: June 30, 2016Applicant: Monsanto Technology LLCInventors: Edwards M. Allen, Sara E. Heisel, Sergey Ivashuta, Elysia K. Krieger, Jennifer Lutke, Robert J. Meister, Yuanji Zhang
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Patent number: 9315819Abstract: This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.Type: GrantFiled: February 27, 2014Date of Patent: April 19, 2016Assignee: Monsanto Technology LLCInventors: Edwards M. Allen, Sara E. Heisel, Sergey Ivashuta, Elysia K. Krieger, Jennifer Lutke, Robert J. Meister, Yuanji Zhang
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Patent number: 9212370Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.Type: GrantFiled: March 18, 2010Date of Patent: December 15, 2015Assignee: Monsanto Technology LLCInventors: Edwards M. Allen, Larry A. Gilbertson, Sara E. Heisel, Shihshieh Huang, Elysia K. Krieger, Thomas M. Malvar
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Publication number: 20150184179Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.Type: ApplicationFiled: December 24, 2014Publication date: July 2, 2015Applicant: MONSANTO TECHNOLOGY LLCInventors: Edwards M. Allen, Larry A. Gilbertson, Sara E. Heisel, Shihshieh Huang, Elysia K. Krieger, Thomas M. Malvar
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Publication number: 20150166993Abstract: This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.Type: ApplicationFiled: December 11, 2014Publication date: June 18, 2015Applicant: MONSANTO TECHNOLOGY LLCInventors: Edwards M. Allen, Barry S. Goldman, Liang Guo, Sara E. Heisel, Shihshieh Huang, Sergey I. Ivashuta, David K. Kovalic, Elysia K. Krieger, James K. Roberts, Yuanji Zhang
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Patent number: 9000264Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.Type: GrantFiled: July 8, 2010Date of Patent: April 7, 2015Assignee: Monsanto Technology LLCInventors: Edwards M. Allen, Larry A. Gilbertson, Nancy M. Houmard, Shihshieh Huang, Elysia K. Krieger, Thomas M. Malvar
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Patent number: 8946511Abstract: This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.Type: GrantFiled: October 12, 2007Date of Patent: February 3, 2015Assignee: Monsanto Technology LLCInventors: Edwards Allen, Barry S. Goldman, Liang Guo, Sara E. Heisel, Shihshieh Huang, Sergey I. Ivashuta, David K. Kovalik, Elysia K. Krieger, James K. Roberts, Yuanji Zhang
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Publication number: 20140317780Abstract: This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.Type: ApplicationFiled: February 27, 2014Publication date: October 23, 2014Applicant: Monsanto Technology LLCInventors: Edwards M. Allen, Sara E. Heisel, Sergey Ivashuta, Elysia K. Krieger, Jennifer Lutke, Robert J. Meister, Yuanji Zhang
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Patent number: 8415527Abstract: The present invention has incorporated a non-lethal negative selectable marker gene into the vector backbone DNA of a DNA plasmid used to transform plant cells. These transgenes are designed to express a non-lethal gene product in plant cells that contain the vector backbone DNA of the DNA plasmid. The gene products of the non-lethal negative selectable marker gene are involved in plant hormone biosynthesis pathways, plant hormone substrate diversion, plant hormone degradation, plant hormone signaling or metabolic interference. The use of these DNA plasmids to transform plant cells provides for the enhanced production of commercially viable plants.Type: GrantFiled: August 17, 2009Date of Patent: April 9, 2013Assignee: Monsanto Technology LLCInventors: Larry A. Gilbertson, Elysia K. Krieger, Wanggen Zhang, Xudong Ye