Abstract: Disclosed is a microfluidic device implanted in an ex-vivo skin explant to control perfusion, diffusion and collection of molecules over long periods. Also disclosed is a method for assessing permeation or infusion of a biomarker of interest through the skin and to a method for detecting and/or quantifying a biomarker of interest contained in the liquid secreted by the ex-vivo skin explant.

Abstract: Disclosed is an ex vivo model for subcutaneous injection and aims at providing an in vitro method including the steps of i) immersing a skin explant in a solidifiable liquid matrix such that the upper face of the epidermis is not covered, which matrix is itself contained in a cell culture insert, the bottom of which consists of a porous membrane, and (ii) solidifying this matrix so as to trap the immersed portion of this skin explant, wherein the upper face of the epidermis is not covered, and adhering this same matrix to the side walls and the porous membrane of the insert, wherein the skin explant includes a thickness of at least 5 mm of hypodermis.