Abstract: A passive milk collection device for storing milk comprises a flexible inner member including a nipple hole for receiving a nipple, and in which, in use, part of the rear surface of the inner member is configured to securely seal onto a user's breast. The device includes a removable rigid outer member configured to attach onto the front surface of the inner member and to provide a free air space around the nipple area for storing milk from the nipple. The circumference of the outer edge of the rear surface of the flexible inner member is substantially larger than the circumference of the rigid outer member.

Abstract: The present invention provides a method of engineering a cell, comprising the steps of introducing into the cell: i) a site-directed nuclease which is capable of cleaving a target nucleotide sequence at the 3?-end of Intron 1 of a Cytotoxic T-Lymphocyte Associated Protein 4 gene (CTLA4); and ii) a nucleic acid construct which comprises a nucleic acid sequence comprising one or more of exon 2, exon 3 and exon 4 of CTLA4, or a sequence with at least 70% identity to exon 2, exon 3 and/or exon 4 of the CTLA4 gene, and 5?- and 3?-homology arms, wherein each of the 5? and '3 homology arms is essentially complementary to a sequence flanking the target nucleotide sequence at the 3?-end of Intron 1 of CTLA4.

Abstract: A vector comprising a first polynucleotide encoding a FOXP3 polypeptide and a second polynucleotide encoding a chimeric antigen receptor (CAR), wherein the CAR comprises an antigen recognition domain which specifically binds to a human leukocyte antigen (HLA), wherein the first polynucleotide and the second polynucleotide are operably linked to the same promoter, and wherein the first polynucleotide is upstream of the second polynucleotide.

Abstract: A passive milk collection device for storing milk comprises a flexible inner member including a nipple hole for receiving a nipple, and in which, in use, part of the rear surface of the inner member is configured to securely seal onto a user's breast. The device includes a removable rigid outer member configured to attach onto the front surface of the inner member and to provide a free air space around the nipple area for storing milk from the nipple. The circumference of the outer edge of the rear surface of the flexible inner member is substantially larger than the circumference of the rigid outer member.

Abstract: Use of C-X-C chemokine receptor type 4 (CXCR4) for increasing the capacity for self-renewal and/or persistence in a T cell; increasing the capacity for engraftment in a T cell; and/or increasing the memory function of a T cell.