Patents by Inventor Encarnacion Mellado Duran

Encarnacion Mellado Duran has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Enzymatic process for the preparation of cephalosporanic 7$B(G)-(4-Carboxybutanamide) acid by means of the modified enzyme D-aminoacid oxidase of trigonopsis variabilis produced in escherichia coli
    Patent number: 6635458
    Abstract: Enzymatic process for the preparation of cephalosporanic 7&bgr;-(4-carboxybutanamide) acid by using the modified enzyme D-aminoacid oxidase of Trigonopsis variabilis produced in Escherichia coli. The process for the expression of the enzyme comprises: (I) isolating the DNA corresponding to gene which codes for the enzyme D-aminoacid oxidase; (II) removing the intron which is contained in said gene; (III) inserting the DNA fragment obtained into the plasmide which is capable of replication in Escherichia coli; (IV) fusing at the extremity 5′ of the structural region of the gene a synthetic assembler which contains a nucleotide sequence which codes for six histidines; (V) transforming a strain of Escherichia coli with the resulting recombinant plasmide; (VI) cultivating the transformed cells of Escherichia coli; and (VII) recovering the enzyme D-aminoacid oxidase of the former cultivation operation through affinity chromatography.
    Type: Grant
    Filed: July 15, 1999
    Date of Patent: October 21, 2003
    Assignee: Antibioticos, S.A.U.
    Inventors: Jose Luis Garcia Lopez, Estrella Cortes Rubio, Jorge Alonso Palacios, Encarnacion Mellado Duran, Bruno Diez Garcia, Jose Manuel Guisan Seijas, Franciso Salto Maldonado, Barredo Fuente
  • ENZYMATIC PROCESS FOR THE PREPARATION OF CEPHALOSPORANIC 7$B(G)-(4-CARBOXYBUTANAMIDE) ACID BY MEANS OF THE MODIFIED ENZYME D-AMINOACID OXIDASE OF TRIGONOPSIS VARIABILIS PRODUCED IN ESCHERICHIA COLI
    Publication number: 20030119087
    Abstract: Enzymatic process for the preparation of cephalosporanic 7?-(4-carboxybutananude) acid by using the modified enzyme D-aminoacid oxidase of Trigonopsis variabilis produced in Escherichia coli. The process for the expression of the enzyme comprises: (I) isolating the DNA corresponding to gene which codes for the enzyme D-aminoacid oxidase; (II) removing the intron which is contained in said gene; (III) inserting the DNA fragment obtained into the plasmide which is capable of replication in Escherichia coli; (IV) fusing at the extremity 5′ of the structural region of the gene a synthetic assembler which contains a nucleotide sequence which codes for six histidines; (V) transforming a strain of Escherichia coli with the resulting recombinant plasmide; (VI) cultivating the transformed cells of Escherichia coli; and (VII) recovering the enzyme D-aminoacid oxidase of the former cultivation operation through affinity chromatography.
    Type: Application
    Filed: July 15, 1999
    Publication date: June 26, 2003
    Inventors: JOSE LUIS GARCIA LOPEZ, ESTRELLA CORTES RUBIO, JORGE ALONSO PALACIOS, ENCARNACION MELLADO DURAN, BRUNO DIEZ GARCIA, JOSE MANUEL GUISAN SEIJAS, FRANCISO SALTO MALDONADO, BARREDO FUENTE