Abstract: This invention relates to an isolated nucleic acid fragment encoding a viral movement protein. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the viral movement protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the viral movement protein in a transformed host cell.

Abstract: This invention relates to an isolated nucleic acid fragment encoding a viral movement protein. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the viral movement protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the viral movement protein in a transformed host cell.

Abstract: Methods and compositions are provided for reducing the level of expression of a target polynucleotide in an organism. The methods and compositions selectively silence the target polynucleotide through the expression of a chimeric polynucleotide comprising the target for a sRNA (the trigger sequence) operably linked to a sequence corresponding to all or part of the gene or genes to be silenced. In this manner, the final target of silencing is an endogenous gene in the organism in which the chimeric polynucleotide is expressed. In a further embodiment, the miRNA target is that of a heterologous miRNA or siRNA, the latter of which is coexpressed in the cells at the appropriate developmental stage to provide silencing of the final target when and where desired. In a further embodiment, the final target may be a gene in a second organism, such as a plant pest, that feeds upon the organism containing the chimeric gene or genes.

Abstract: This invention relates to an isolated nucleic acid fragment encoding a viral movement protein. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the viral movement protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the viral movement protein in a transformed host cell.

Abstract: This invention relates to isolated nucleic acid fragments encoding all or a substantial portion of a corn, rice, wheat or soybean 4-&agr;-glucanotransferase. The invention also relates to the construction of chimeric genes encoding all or a portion of a corn, rice, wheat or soybean 4-&agr;-glucanotransferase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of 4-&agr;-glucanotransferase in a transformed host cell.

Abstract: A plant consisting essentially of cells which comprise in their genome a homozygous male-sterility genotype at a first genetic locus; and a color-linked restorer genotype at a second genetic locus, which is heterozygous (Rf/-) for a foreign DNA Rf. The foreign DNA Rf comprises: a) a fertility-restorer gene capable of preventing the phenotypic expression of the male-sterility genotype, and b) at least one anthocyanin regulatory gene involved in the regulation of anthocyanin biosynthesis in cells of seeds of the plant which is capable of producing anthocyanin at least in the seeds of the plant, so that anthocyanin production in the seeds is visible externally. Preferably, the anthocyanin regulatory gene is a shortened R, B or C1 gene or a continuation thereof.

Abstract: This invention relates to isolated nucleic acid fragments encoding all or a substantial portion of a corn 4-.alpha.-glucanotransferase. The invention also relates to the construction of chimeric genes encoding all or a portion of a corn 4-.alpha.-glucanotransferase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of corn 4-.alpha.-glucanotransferase in a transformed host cell.

Abstract: This invention is directed to a method to maintain male sterility in corn by the genetic linkage via genetic engineering of a homozygous male-fertility gene with a color linked restorer and plant comprising said genes. Color genes disclosed as useful in this invention include those involved in anthocyanin biosynthesis, which in corn are under the control of as many as 20 or more genes. In particular, the use of the anthocyanin color genes R, B and C1, and specific regulatory elements sequences are taught.

Abstract: A seed-specific promoter is described which is obtained from an Arabidopsis seed storage protein gene encoding 2S albumin.

Abstract: A process for the production of plants with increased content of appropriate amino acids having high nutritional properties through the modification of plant genes encoding plant storage proteins, particularly the 2S albumins. Seed-forming plants susceptible to Agrobacterium transformation, the genome of which plants includes a recombinant DNA encoding a precursor of a modified 2S albumin, under the control of a promoter, are described, including the particular recombinant DNA.

Abstract: A process is described for producing a polypeptide by cultivating a plant whose genome contains a recombinant DNA. The recombinant DNA includes:a) a first DNA sequence which encodes a precursor of a 2S albumin; andb) a heterologous second DNA sequence that encodes the polypeptide and that is inserted into, or replaces at least in part, a hypervariable region of the first DNA between codons which code for fourth and fifth cysteine residues of the large subunit of the 2S albumin. The ends of the second DNA sequence are each linked to one or more codons that encode one or more amino acid residues, to define first selectively cleavable border sites surrounding the polypeptide for separating the polypeptide from surrounding parts of the 2S albumin; and the hypervariable region, containing the second DNA sequence, encodes no more than approximately the same number of amino acids as are encoded by the hypervariable region without the second DNA sequence.