Abstract: Provided is an application of a sphere substance in the preparation of an intravenous injection, relating to the technical field of biomedicine. Intravenous injection of the sphere substance formed by MSC spheres or biomaterials is used as a new in-vivo delivery solution. Compared with the intravenous injection of discrete MSCs, the intravenous injection of the MSC spheres reduces the retention of the MSCs in lungs, and prolongs the-retention time and activity of the MSCs in blood and peripheral tissues, such that therapeutic effects for autoimmune diseases, inflammatory diseases, and tissues and organ injuries can be improved. In addition, the intravenous injection of biomaterial spheres is safe, can carry drugs, biologically active molecules or detection molecules, is applied to diagnosis and treatment of an animal disease model or a patient, is more effective and more durable than monomer molecules, and can also carry a variety of molecules to generate a synergistic effect.

Abstract: The present disclosure discloses use of mesenchymal stem cells in preparation of a formulation for promoting fat transplantation, relating to the field of biotechnologies. The inventors found through research that compared with MSCs derived from somatic cells, MSCs derived from human pluripotent stem cells have more stable quality, are not affected by donor's physical quality, disease and treatment process, and can promote fat transplantation by enhancing tissue remodeling, angiogenesis and adipose cell survival and decreasing tissue fibrosis.

Abstract: Disclosed are a device for preparing stem cell spheroids, a method for preparing stem cell spheroids and a method for preserving stem cells which relate to the technical field of preservation and transportation of stem cells. The device for preparing stem cell spheroids disclosed herein includes a substrate. The substrate is provided thereon with culture chambers, each of which has side walls and a bottom wall made of a material incompatible with stem cells. By culturing stem cells with this device, the stem cells may be made into spheroids and thus form stem cell spheroids. The device may be used as a means of preserving or transporting stem cells which makes it possible for stem cells to still have a higher viability and pluripotency even after a long time of preservation, storage and transportation under ambient temperature condition in the form of spheroids.

Abstract: A culture method for enhancing the activity of stem cells, and a method of preparing a formulation for treating central nervous diseases, and a method of treating central nervous diseases are provided. In some aspects, stem cell clusters are cultured for 3 to 11 days in a sealed container filled with a culture medium, under a condition in which no oxygen and carbon dioxide are supplied. By placing stem cells in a sealed container to stabilize the properties and enhance the functions thereof under a room temperature condition, the stemness and apoptosis resistance of the stem cells are improved, thereby significantly improving the therapeutic effect of the stem cells after transplantation into a human body.