Abstract: A method for determining in a sample solution the presence of a target nucleic acid of interest, where the target nucleic acid of interest differs from one or more other target nucleic acids by only one or two nucleotides. The method comprises providing a first plurality of different probes and a second plurality of different probes, performing hybridization, where the hybridization includes contacting the sample solution with each probe of the first and second plurality of probes, and detecting a hybridization signal for each probe with the sample solution, determining the hybridization free energy for the hybridization between each probe of the first and second plurality of probes and the target nucleic acid of interest, and determining the presence of the target nucleic acid of interest based on the relationship between the hybridization signals detected for the plurality of probes.

Abstract: A method is described for the analysis of hybridisation between a target in solution and a probe bound at a surface. The method comprises receiving detection intensity results for hybridisation of the target with a plurality of different probes, the probes being selected so that a range of hybridisation detection intensity results for the hybridisation between the target and the probe is covered. The method further comprises analysing the detection intensity results as function of the hybridisation free energy. According to embodiments of the present invention, the receiving and/or analysing takes into consideration a thermodynamic non-equilibrium state for the target-probe bounding state.

Abstract: A method is described for the analysis of hybridisation between a target in solution and a probe bound at a surface. The method comprises receiving detection intensity results for hybridisation of the target with a plurality of different probes, the probes being selected so that a range of hybridisation detection intensity results for the hybridisation between the target and the probe is covered. The method further comprises analysing the detection intensity results as function of the hybridisation free energy. According to embodiments of the present invention, the receiving and/or analysing takes into consideration a thermodynamic non-equilibrium state for the target-probe bounding state.