Abstract: Novel peptides which are conformationally constrained backbone cyclized antagonists of IL-6, are disclosed. Methods for synthesizing the IL-6 antagonists are also disclosed. Furthermore, pharmaceutical compositions comprising IL-6 antagonists, and methods of using such compositions are disclosed.

Abstract: The hybridoma 34-1 (CNCM I-813) which produces anti-human IL-6 monoclonal antibody 34-1 is disclosed. Methods of producing the monoclonal antibody and methods for affinity purification of human IL-6 using monoclonal antibody 34-1 are also described.

Abstract: A single-step procedure for the immobilization of antibodies, comprising reacting a purified antibody with buffered periodate in an amount sufficient to produce aldehyde groups in the presence of a polymer carrier material which has been modified with adipic acid dihydrazide is disclosed. The present invention provides dramatic improvements in activity. and yield of immobilized antibody over the prior multi-step procedure employing separate oxidation and immobilization steps.

Abstract: Antibodies which are immobilized and covalently bound to a matrix polymer by means of a modification in a carbohydrate region of the antibodies; wherein the binding of the antibodies is effected by condensing at least one aldehyde group in an oxidized carbohydrate region and at least one epoxide function of an epoxy-group-containing matrix polymer, said condensation being conducted in the presence of a bifunctional reagent which has, on one end position of a spacer unit having at least three members, an amino group capable of condensing with the aldehyde group, and which has on the other end position a group which reacts covalently with the epoxide function.