Abstract: Methods of determining a therapeutic response to immunotherapy in a subject suffering from lung cancer are provided. Kits for use in determining a therapeutic response to immunotherapy are also provided.

Abstract: A method comprising receiving, for each of a plurality of subjects having a specified type of disease and receiving a specified therapy for treating the disease, a first biological signature obtained pre-treatment and a second biological signature obtained on-treatment; calculating, for each of the plurality of subjects, a set of values representing a ratio between the first and second biological signatures associated with the respective subject; at a training stage, training a machine learning model on a training set comprising: (i) the calculated sets of values, and (ii) labels associated with an outcome of the specified therapy in each of the subjects; to generate a classifier suitable for predicting a response in a target patient to said specified therapy.