Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain low error rate, low phasing rate, or increased incorporation rate for a second generation ffN under reduced incorporation times, as well as methods and kits using the same.

Abstract: A reel for tightening an article includes a housing and a spool positioned within the housing. A knob is coupled with the spool to cause the spool to rotate within the housing and thereby wind a tension member about a central post of the spool. A load holding mechanism is coupled with the spool and the housing and includes a first friction element and a second friction element that are frictionally engageable to prevent rotation of the spool in a second direction to prevent unwinding of the tension member from the spool's central post. Rotation of the knob in a first direction reduces frictional engagement of the friction elements to enable rotation of the spool in the first direction and tension in the tension member biases the spool toward rotation in the second direction which increases the frictional engagement of the friction elements.

Abstract: A reel for tightening an article includes a housing and a spool positioned within the housing. A knob is coupled with the spool to cause the spool to rotate within the housing and thereby wind a tension member about a central post of the spool. A load holding mechanism is coupled with the spool and the housing and includes a first friction element and a second friction element that are frictionally engageable to prevent rotation of the spool in a second direction to prevent unwinding of the tension member from the spool's central post. Rotation of the knob in a first direction reduces frictional engagement of the friction elements to enable rotation of the spool in the first direction and tension in the tension member biases the spool toward rotation in the second direction which increases the frictional engagement of the friction elements.

Abstract: A reel for tightening an article includes a housing and a spool positioned within the housing. A knob is coupled with the spool to cause the spool to rotate within the housing and thereby wind a tension member about a central post of the spool. A load holding mechanism is coupled with the spool and the housing and includes a first friction element and a second friction element that are frictionally engageable to prevent rotation of the spool in a second direction to prevent unwinding of the tension member from the spool's central post. Rotation of the knob in a first direction reduces frictional engagement of the friction elements to enable rotation of the spool in the first direction and tension in the tension member biases the spool toward rotation in the second direction which increases the frictional engagement of the friction elements.

Abstract: A reel for tightening an article includes a housing and a spool positioned within the housing. A knob is coupled with the spool to cause the spool to rotate within the housing and thereby wind a tension member about a central post of the spool. A load holding mechanism is coupled with the spool and the housing and includes a first friction element and a second friction element that are frictionally engageable to prevent rotation of the spool in a second direction to prevent unwinding of the tension member from the spool's central post. Rotation of the knob in a first direction reduces frictional engagement of the friction elements to enable rotation of the spool in the first direction and tension in the tension member biases the spool toward rotation in the second direction which increases the frictional engagement of the friction elements.

Abstract: A reel based lacing system is configured to allow the incremental tightening of a lace about a spool by rotation of a knob in the tightening direction. In some embodiments, the system can include a substantially inflexible pawl beam configured to resist rotation of the knob in the loosening direction and a pawl spring configured to bias the pawl against the housing and to allow the pawl to be displaced away from the housing when the knob is rotated in the tightening direction.

Abstract: This disclosure relates to articles that include a tightening mechanism, such as reel-based lace tightening mechanism, configured to tighten the article by rotation of a knob. The articles can include a concealing portion that is configured to conceal or protect at least a portion of the tightening mechanism, such as the knob. The concealing portion can be configured to prevent unintentional actuation of the tightening mechanism, such as during contact sports. The concealing portion can be configured to hide the tightening mechanism from view to improve the visual appearance of the article. The concealing portion can be collapsible such that a user can press the concealing portion down to expose the knob of the tightening mechanism.

Abstract: A reel for tightening an article includes a housing and a spool positioned within the housing. A knob is coupled with the spool to cause the spool to rotate within the housing and thereby wind a tension member about a central post of the spool. A load holding mechanism is coupled with the spool and the housing and includes a first friction element and a second friction element that are frictionally engageable to prevent rotation of the spool in a second direction to prevent unwinding of the tension member from the spool's central post. Rotation of the knob in a first direction reduces frictional engagement of the friction elements to enable rotation of the spool in the first direction and tension in the tension member biases the spool toward rotation in the second direction which increases the frictional engagement of the friction elements.

Abstract: A reel based lacing system is disclosed. The reel can be configured to allow the incremental tightening of a lace about a spool by rotation of a knob in the tightening direction. In some embodiments, the system can include a substantially inflexible pawl beam configured to resist rotation of the knob in the loosening direction and a pawl spring configured to bias the pawl against the housing and to allow the pawl to be displaced away from the housing when the knob is rotated in the tightening direction.

Abstract: Microwaveable popcorn arrangements are provided, the arrangement being a bag having a popcorn charge of popcorn kernels and oil/fat component therein. The arrangements include folded bags having preferred, internal, seal and/or adhesive field configurations. The preferred configurations inhibit the undesired flow of oil/fat from the popcorn charge. Microwave popcorn products and methods of preparation and use, are provided.

Abstract: This disclosure relates to articles that include a tightening mechanism, such as reel-based lace tightening mechanism, configured to tighten the article by rotation of a knob. The articles can include a concealing portion that is configured to conceal or protect at least a portion of the tightening mechanism, such as the knob. The concealing portion can be configured to prevent unintentional actuation of the tightening mechanism, such as during contact sports. The concealing portion can be configured to hide the tightening mechanism from view to improve the visual appearance of the article. The concealing portion can be collapsible such that a user can press the concealing portion down to expose the knob of the tightening mechanism.

Abstract: A reel based lacing system is disclosed. The reel can be configured to allow the incremental tightening of a lace about a spool by rotation of a knob in the tightening direction. In some embodiments, the system can include a substantially inflexible pawl beam configured to resist rotation of the knob in the loosening direction and a pawl spring configured to bias the pawl against the housing and to allow the pawl to be displaced away from the housing when the knob is rotated in the tightening direction.

Abstract: By way of example, a cam assembly and strap based closure system using a spiral is configured for bringing two sides of an article together. When the user inserts the strap into the cam assembly and turns a knob, the strap is driven into the cam assembly and the strap pins engage one or more cam spirals. The system is configured such that strap pins are engaged at a constant angle which may be self-locking. The system is infinitely adjustable and the torque felt by the knob is constant. The system is configured to be a quick release system and allows rapid insertion of the strap for faster operation.

Abstract: There is provided headwear comprising, a crown defining an opening for receiving a head of a wearer, an actuator configured to receive an application of a force, and a force transmission member coupled to the actuator. The actuator is configured to effect, when a head is received within the crown, and relative to the head received within the crown, a change in disposition of the force transmission member to any one of a plurality of conditions in response to an application of force to the actuator, wherein, in at least one of the plurality of conditions, an operative surface of the force transmission member is bearing against a surface fraction of the head received within the crown.

Abstract: A system and method for exercising core muscles, particularly the lumbar intrinsic musculature, including the multifidi. A system is disclosed that includes: a first sensor for detecting upper body exertions of a user engaged in an exercise; a second sensor for detecting lower torso exertions for the user engaged in the exercise; a third sensor for detecting lower extremity exertions for the user engaged in the exercise; a control system for processing sensor data from the first, second and third sensor, said control system including: a user interface system for communicating information with the user; a data collection system for collecting sensor data; an analysis system for analyzing the sensor data and determining if the user is performing the exercise in a technically correct manner; and a feedback system for alerting the user when the exercise is not being performed in the technically correct manner.

Abstract: A reel based lacing system is disclosed. The reel can be configured to allow the incremental tightening of a lace about a spool by rotation of a knob in the tightening direction. In some embodiments, the system can include a substantially inflexible pawl beam configured to resist rotation of the knob in the loosening direction and a pawl spring configured to bias the pawl against the housing and to allow the pawl to be displaced away from the housing when the knob is rotated in the tightening direction.

Abstract: There is provided headwear comprising, a crown defining an opening for receiving a head of a wearer, an actuator configured to receive an application of a force, and a force transmission member coupled to the actuator. The actuator is configured to effect, when a head is received within the crown, and relative to the head received within the crown, a change in disposition of the force transmission member to any one of a plurality of conditions in response to an application of force to the actuator, wherein, in at least one of the plurality of conditions, an operative surface of the force transmission member is bearing against a surface fraction of the head received within the crown.

Abstract: Microwaveable popcorn arrangements are provided, the arrangement being a bag having a popcorn charge of popcorn kernels and oil/fat component therein. The arrangements include folded bags having preferred, internal, seal and/or adhesive field configurations. The preferred configurations inhibit the undesired flow of oil/fat from the popcorn charge. Microwave popcorn products and methods of preparation and use, are provided.

Abstract: Microwaveable popcorn arrangements are provided, the arrangement being a bag having a popcorn charge of popcorn kernels and oil/fat component therein. The arrangements include folded bags having preferred, internal, seal and/or adhesive field configurations. The preferred configurations inhibit the undesired flow of oil/fat from the popcorn charge. Microwave popcorn products and methods of preparation and use, are provided.