Abstract: The development of miniaturized chromatographic systems localized within individual polymer microspheres and their incorporation into a bead-based cross-reactive sensor array platform is described herein. The integrated chromatographic and detection concept is based on the creation of distinct functional layers within the microspheres. In this first example of the new methodology, complexing ligands have been selectively immobilized to create “separation” layers harboring an affinity for various analytes. Information concerning the identities and concentrations of analytes may be drawn from the temporal properties of the beads' optical responses. Varying the nature of the ligand in the separation shell yields a collection of cross-reactive sensing elements well suited for use in array-based micro-total-analysis systems.

Abstract: Apparatus for receiving and positioning optical components. The apparatus includes a substrate, one or more mounting slots, and one or more springs. The one or more mounting slots are formed in the substrate, and each mounting slot includes a mounting slot wall. At least one of the mounting slots is adapted to receive an optical component. At least one of the mounting slots is coupled to one of the springs.

Abstract: Synthetic receptor cores are provided that comprise a compound represented by the following formula: wherein R1, R2, and R3 independently comprise at least one moiety chosen from a hydroxyl group, an amine group, and derivatives thereof. Synthetic receptor cores are provided that comprise a compound represented by the following formula: wherein R comprises at least one moiety chosen from a hydroxyl group, an amine group, and derivatives thereof. Synthetic receptors are provided that comprise a synthetic receptor core; and an analyte binding moiety, wherein the analyte binding moiety is capable of complexion with an analyte. Systems, methods, and kits are also provided that use a synthetic receptor.

Abstract: The invention includes an enantioselective indicator-displacement assay useful to determine enantiomeric excess (ee) enantiomeric samples calorimetrically. Determination may be by inspection of color with the naked eye, spectrographic measurement, or mathematical calculation. Concentration may also be determined. The assay may involve two independent absorption measurements. On suitable group of enantiomeric molecules to be assayed include ?-hydroxyacids. The inherent relationship between the absorbance of the indicator-displacement ensemble and the overall concentration and ee of the analyte is established through solution equilibria. The invention also includes use of the assay in drug screening and manufacturing, high throughput screening of catalysts and kits for use in conducting assays of the invention.

Abstract: A system for the rapid characterization of analytes in saliva. In one embodiment, a system for detecting analytes includes a light source, a sensor array, and a detector. The sensor array is formed from a supporting member, in which a plurality of cavities may be formed. A series of chemically sensitive particles, in one embodiment, are positioned within the cavities. The particles may produce a signal when a receptor, coupled to the particle, interacts with the cardiovascular risk factor analyte and the particle-analyte complex is visualized using a visualization reagent. Using pattern recognition techniques, the analytes within a multi-analyte fluid may be characterized. In an embodiment, each cavity of the plurality of cavities is designed to capture and contain a specific size particle. Flexible projections may be positioned over each of the cavities to provide retention of the particles in the cavities.

Abstract: A system for the rapid characterization of multi-analyte fluids, in one embodiment, includes a light source, a sensor array, and a detector. The sensor array is formed from a supporting member into which a plurality of cavities may be formed. A series of chemically sensitive particles are, in one embodiment positioned within the cavities. The particles may be configured to produce a signal when a receptor coupled to the particle interacts with the analyte. Using pattern recognition techniques, the analytes within a multi-analyte fluid may be characterized.

Abstract: A system for the rapid characterization of multi-analyte fluids, in one embodiment, includes a light source, a sensor array, and a detector. The sensor array is formed from a supporting member into which a plurality of cavities may be formed. A series of chemically sensitive particles are, in one embodiment positioned within the cavities. The particles may be configured to produce a signal when a receptor coupled to the particle interacts with the analyte. Using pattern recognition techniques, the analytes within a multi-analyte fluid may be characterized.

Abstract: Apparatus for receiving and positioning optical components. The apparatus includes a substrate, one or more mounting slots, and one or more springs. The one or more mounting slots are formed in the substrate, and each mounting slot includes a mounting slot wall. At least one of the mounting slots is adapted to receive an optical component. At least one of the mounting slots is coupled to one of the springs.