Abstract: A sealed system for handling, manipulating and formulating materials in an isolated environment which includes an isolation system for performing operations on the materials, an accessory for receiving, performing a procedure, and providing items, and a sealing device interconnecting the isolation system and the accessory. The isolation system has an interior at least partially defined by a plurality of walls and a transfer port positioned on one of the walls for transferring items into and out of the isolation system. The accessory is selectively coupled to the transfer port and has an interior accessed by an opening. The sealing device includes a trough of predetermined configuration and a blade of corresponding configuration. The trough has a sealing substance disposed therein, and the blade and the trough each surround one of the transfer port and the opening. The blade is sealingly mounted to a mounting member which is sealingly mounted to the accessory to seal the interior of the accessory.