Abstract: A method for the detection of minority genomes in virus quasispecies using DNA microchips. The method makes it possible to detect minority genomes, more particularly minority memory genomes, in a nucleic acids population of a virus quasispecie, which are present in a population of less than 50%, containing at least one mutation relative to the majority genomes of the quasispecie. The method involves the following steps: a) extracting the nucleic acid of the virus quasispecie form a sample susceptible of containing the virus quasispecie; b) amplifying at least one fragment of the nucleic acid of the virus quasispecie, and c) detecting and analyzing the existence of minority genomes using DNA microchip-base techniques. The method can be used in genetic diagnosis of viral diseases.