Abstract: Described is a lateral flow high throughput assay device analyzer for preparing and analyzing a plurality of lateral flow assay samples. The analyzer comprises a cartridge stage for supporting an assay cartridge, an elevation adjustment mechanism, and a translation adjustment mechanism for aligning the cartridge stage and assay cartridge relative to a vertical support structure, fluid metering device, and detection device for high throughput lateral flow assay analysis.

Abstract: A method and system for subtractive patterning of a substrate, which is utilized in the making of paper-based microfluidic analytical devices (pPADs). By adhering the substrate on an impermeable backing material, the substrate is etched to yield high resolution features, which can be utilized to construct MPADS capable of flowing and testing extremely small sample volumes. This system and method can be modified for various substrates to construct features for two and three dimensional flow systems. A substrate assembly is formed by affixing a substrate layer (e.g. paper) to an impermeable layer (e.g. foil). Portions of the substrate layer are cut away using an etching device to form one or more subtractive patterns on the substrate assembly the define fluid flow regions.