Abstract: Aspects describe nanoparticle-delivered adenyl cyclase activators (e.g., forskolin via LCMSN) to induce sWAT browning, enhancing thermogenesis for wound healing and cold injury protection. Applications include chronic wounds, diabetic ulcers, burns, frostbite, and hypothermia via subcutaneous or transdermal routes, with demonstrated acceleration of closure, reduced inflammation/necrosis, and improved angiogenesis/ECM remodeling in frostbite models.

Abstract: Methods and compositions related to thermogenic adipose for study and therapeutics are described. Certain embodiments are directed to vascularized thermogenic adipose tissue developed using microvascular fragments (MVFs). MVFs are isolated and thermogenic adipose formed.

Abstract: A thermo-responsive hydrogel, including a biocompatible monomer and/or polymer having a side chain-linked amino acid. The hydrogel is thermo-responsive at a physiological temperature, and can include, incorporate, or encapsulate a treatment agent, such as a drug composition, a biomolecule, and/or a nano-particle. The hydrogel is useful in delivering the treatment agent. The hydrogel is in a first physicochemical state for administration to a mammal. The hydrogel is thermo-responsive at a physiological temperature of the mammal, and changes to a second physicochemical state that is more solid than the first physicochemical state. In the second physicochemical state the thermo-responsive hydrogel releases the treatment agent.

Abstract: A thermo-responsive hydrogel, including a biocompatible monomer and/or polymer having an amino acid side chain. The hydrogel is thermo-responsive at a physiological temperature, and can include, incorporate, or encapsulate a treatment agent, such as a drug composition, a biomolecule, and/or a nanoparticle. The hydrogel is useful in delivering the treatment agent. The hydrogel is in a first physicochemical state for administration to a mammal. The hydrogel is thermo-responsive at a physiological temperature of the mammal, and changes to a second physicochemical state that is more solid than the first physicochemical state. In the second physicochemical state the thermo-responsive hydrogel releases the treatment agent.

Abstract: A wound healing chamber is disclosed, which includes: a fixing plate with a first opening, wherein suturing holes are circularly formed around a peripheral section of the first opening, and the fixing plate is fixed on an object through the suturing holes; and a detachable cap mounted on the fixing plate, wherein the cap corresponds to the first opening, and the first opening is open or closed by the detachable cap.

Abstract: Methods are provided for basement membrane extraction and gellation whereby a biologically active, stable, three dimensional gel can be induced within five minutes or less with basement membrane components from substantially any tissue selected by the preparer. The three dimensional gel can further be substantially free of cellular contaminants. Thus, a wide range of diagnostic and therapeutic uses for such gels can be made available.