Abstract: There is provided a fusion construct of formula (I): X-A-linker-B-Z (I) wherein: (1) A and B are identical or different and are independently: (a) a polypeptide comprising a SACOL0029 polypeptide as set forth in any one of the sequences depicted in FIG. 24 (SEQ ID NOs: 5 and 121 to 131), a SACOL264 polypeptide (SEQ ID NO: 185), a SACOL0442 polypeptide as set forth in any one of the sequences depicted in FIG. 22D (SEQ ID NOs: 29 and 82 to 92), a SACOL0718 polypeptide (SEQ ID NO: 186), a SACOL0720 polypeptide as set forth in any one of the sequences depicted in FIGS. 23I-J (SEQ ID NOs: 11 and 109 to 120), a SACOL1353 polypeptide (SEQ ID NO: 187), a SACOL1416 polypeptide (SEQ ID NO: 188), a SACOL1611 polypeptide (SEQ ID NO: 189), a SACOL1867 polypeptide as set forth in any one of the sequences depicted in FIG.

Abstract: The present invention relates to the use of a plasmid encoding Staphylococcus aureus polypeptides and its use in the preparation of compositions and vaccines. More specifically, the present invention is concerned with compositions, DNA vaccines and methods for providing an immune response and/or a protective immunity into mammals against a Staphylococcus aureus associated disease, such as mastitis. The plasmid used in the composition or DNA vaccine comprises at least one nucleotide coding sequence of a Staphylococcus aureus polypeptide, such as the Clumping factor A (ClfA), the fibronectin-binding protein A, the sortase-A or the pre-pheromone (ArgD).