Abstract: The present invention relates to the field of pesticides. More specifically, the present invention provides compositions and methods for the preparation of non-live Chromobacterium pesticides. In a specific embodiment, a method for producing a non-live Chromobacterium biopesticide comprises the steps of (a) growing the Chromobacterium in a bioreactor with culture medium; (b) oxygenating the Chromobacterium during step (a); (c) adding an antibiotic to the Chromobacterium culture; (d) incubating the Chromobacterium culture under hypoxic conditions; (e) pouring the Chromobacterium culture on to a substrate; (f) air drying the Chromobacterium; and (g) crushing the dried Chromobacterium into a powder.