Patents by Inventor Eric Endsley
Eric Endsley has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11790527Abstract: Systems and methods are provided for automatically imaging and analyzing cell samples in an incubator. An actuated microscope operates to generate images of samples within wells of a sample container across days, weeks, or months. A plurality of images is generated for each scan of a particular well, and the images within such a scan are used to image and analysis metabolically active cells in the well. Tins analysis includes generating a “range image” by subtracting the minimum intensity value, across the scan, for each pixel from the maximum intensity value. This range image thus emphasizes cells or portions of cells that exhibit changes in activity over a scan period (e.g., neurons, myocytes, cardiomyocytes) while de-emphasizing regions that exhibit consistently high intensities when images (e.g., regions exhibiting a great deal of autofluorescence unrelated to cell activity).Type: GrantFiled: September 8, 2022Date of Patent: October 17, 2023Assignee: Sartorius BioAnalytical Instruments, Inc.Inventors: Daniel Appledorn, Eric Endsley, Nevine Holtz, Brad Neagle, David Rock, Kirk Schroeder
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Publication number: 20230005143Abstract: Systems and methods are provided for automatically imaging and analyzing cell samples in an incubator. An actuated microscope operates to generate images of samples within wells of a sample container across days, weeks, or months. A plurality of images is generated for each scan of a particular well, and the images within such a scan are used to image and analysis metabolically active cells in the well. Tins analysis includes generating a “range image” by subtracting the minimum intensity value, across the scan, for each pixel from the maximum intensity value. This range image thus emphasizes cells or portions of cells that exhibit changes in activity over a scan period (e.g., neurons, myocytes, cardiomyocytes) while de-emphasizing regions that exhibit consistently high intensities when images (e.g., regions exhibiting a great deal of autofluorescence unrelated to cell activity).Type: ApplicationFiled: September 8, 2022Publication date: January 5, 2023Inventors: Daniel APPLEDORN, Eric ENDSLEY, Nevine HOLTZ, Brad NEAGLE, David ROCK, Kirk SCHROEDER
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Patent number: 11481895Abstract: Systems and methods are provided for automatically imaging and analyzing cell samples in an incubator. An actuated microscope operates to generate images of samples within wells of a sample container across days, weeks, or months. A plurality of images is generated for each scan of a particular well, and the images within such a scan are used to image and analysis metabolically active cells in the well. Tins analysis includes generating a “range image” by subtracting the minimum intensity value, across the scan, for each pixel from the maximum intensity value. This range image thus emphasizes cells or portions of cells that exhibit changes in activity over a scan period (e.g., neurons, myocytes, cardiomyocytes) while de-emphasizing regions that exhibit consistently high intensities when images (e.g., regions exhibiting a great deal of autofluorescence unrelated to cell activity).Type: GrantFiled: October 30, 2018Date of Patent: October 25, 2022Assignee: Sartorius BioAnalytical Instruments, Inc.Inventors: Daniel Appledorn, Eric Endsley, Nevine Holtz, Brad Neagle, David Rock, Kirk Schroeder
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Patent number: 11320380Abstract: An imaging apparatus is provided to facilitate epifluorescent imaging of three (or more) color channels and to perform phase contrast and/or bright field imaging of samples without manual adjustment of the imaging apparatus. This allows for automated imaging, over extended periods of time, of a plurality of samples by a device located inside an incubator without disturbing the incubator environment to manually adjust the apparatus. Also provided are embodiments to facilitate user swapping of removable optical modules and/or transillumination modules to allow the imaging apparatus to be adapted to different combinations of assays and/or fluorescent indicators so as to increase the variety of experiments and/or fluorescent dyes that can be imaged using the imaging apparatus.Type: GrantFiled: April 21, 2020Date of Patent: May 3, 2022Assignee: Sartorius BioAnalytical Instruments, Inc.Inventors: Bradley Neagle, Alan Riggs, Michael Kusner, Kyle Schutte, Eric Endsley
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Publication number: 20210325309Abstract: An imaging apparatus is provided to facilitate epifluorescent imaging of three (or more) color channels and to perform phase contrast and/or bright field imaging of samples without manual adjustment of the imaging apparatus. This allows for automated imaging, over extended periods of time, of a plurality of samples by a device located inside an incubator without disturbing the incubator environment to manually adjust the apparatus. Also provided are embodiments to facilitate user swapping of removable optical modules and/or transillumination modules to allow the imaging apparatus to be adapted to different combinations of assays and/or fluorescent indicators so as to increase the variety of experiments and/or fluorescent dyes that can be imaged using the imaging apparatus.Type: ApplicationFiled: April 21, 2020Publication date: October 21, 2021Inventors: Bradley Neagle, Alan Riggs, Michael Kusner, Kyle Schutte, Eric Endsley
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Patent number: 11149242Abstract: Systems, methods, and apparatuses of controlling fluid flow are disclosed. An apparatus includes a first microplate having a first open portion and defining one or more first wells therein, a second microplate having a second open portion and defining one or more second wells therein, and a pneumatic lid constructed of styrene ethylene butylene styrene (SEBS). The pneumatic lid extends over the first open portion and the second open portion and includes one or more microfluidic channels that fluidly couple the one or more first wells to the one or more second wells. The pneumatic lid provides an airtight seal over the first microplate and the second microplate.Type: GrantFiled: March 23, 2020Date of Patent: October 19, 2021Assignees: Essen Instruments, Inc., National Research Council of CanadaInventors: Kirk S. Schroeder, Bradley D. Neagle, Eric Endsley, Daniel Appledorn, Keith Morton
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Publication number: 20210065362Abstract: Systems and methods are provided for automatically imaging and analyzing cell samples in an incubator. An actuated microscope operates to generate images of samples within wells of a sample container across days, weeks, or months. A plurality of images is generated for each scan of a particular well, and the images within such a scan are used to image and analysis metabolically active cells in the well. Tins analysis includes generating a “range image” by subtracting the minimum intensity value, across the scan, for each pixel from the maximum intensity value. This range image thus emphasizes cells or portions of cells that exhibit changes in activity over a scan period (e.g., neurons, myocytes, cardiomyocytes) while de-emphasizing regions that exhibit consistently high intensities when images (e.g., regions exhibiting a great deal of autofluorescence unrelated to cell activity).Type: ApplicationFiled: October 30, 2018Publication date: March 4, 2021Inventors: Daniel APPLEDORN, Eric ENDSLEY, Nevine HOLTZ, Brad NEAGLE, David ROCK, Kirk SCHROEDER
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Publication number: 20200224139Abstract: Systems, methods, and apparatuses of controlling fluid flow are disclosed. An apparatus includes a first microplate having a first open portion and defining one or more first wells therein, a second microplate having a second open portion and defining one or more second wells therein, and a pneumatic lid constructed of styrene ethylene butylene styrene (SEBS). The pneumatic lid extends over the first open portion and the second open portion and includes one or more microfluidic channels that fluidly couple the one or more first wells to the one or more second wells. The pneumatic lid provides an airtight seal over the first microplate and the second microplate.Type: ApplicationFiled: March 23, 2020Publication date: July 16, 2020Inventors: Kirk S. SCHROEDER, Bradley D. NEAGLE, Eric ENDSLEY, Daniel APPLEDORN, Keith MORTON
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Patent number: 10633624Abstract: Systems, methods, and apparatuses of controlling fluid flow are disclosed. An apparatus includes a first microplate having a first open portion and defining one or more first wells therein, a second microplate having a second open portion and defining one or more second wells therein, and a pneumatic lid constructed of styrene ethylene butylene styrene (SEBS). The pneumatic lid extends over the first open portion and the second open portion and includes one or more microfluidic channels that fluidly couple the one or more first wells to the one or more second wells. The pneumatic lid provides an airtight seal over the first microplate and the second microplate.Type: GrantFiled: July 1, 2019Date of Patent: April 28, 2020Assignees: ESSEN INSTRUMENTS, INC., NATIONAL RESEARCH COUNCIL OF CANADAInventors: Kirk S. Schroeder, Bradley D. Neagle, Eric Endsley, Daniel Appledorn, Keith Morton
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Publication number: 20190322972Abstract: Systems, methods, and apparatuses of controlling fluid flow are disclosed. An apparatus includes a first microplate having a first open portion and defining one or more first wells therein, a second microplate having a second open portion and defining one or more second wells therein, and a pneumatic lid constructed of styrene ethylene butylene styrene (SEBS). The pneumatic lid extends over the first open portion and the second open portion and includes one or more microfluidic channels that fluidly couple the one or more first wells to the one or more second wells. The pneumatic lid provides an airtight seal over the first microplate and the second microplate.Type: ApplicationFiled: July 1, 2019Publication date: October 24, 2019Inventors: Kirk S. SCHROEDER, Bradley D. NEAGLE, Eric ENDSLEY, Daniel APPLEDORN, Keith MORTON
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Patent number: 6068073Abstract: A versatile robot provides a plurality of different, primary locomotion means and the capability of switching between modes of movement on a rapid basis to traverse a variety of terrains at high speed. In the preferred embodiment, the robot uses a plurality of legs as a first primary means of locomotion and a plurality of wheels as a second, primary means of locomotion. As such, the robot may use the wheels for high speed locomotion (in excess of 10 mph) over prepared surfaces, and the legs for traversing unprepared surfaces and man-made obstacles at lower speed. The system further employs means for monitoring leg or joint position to determine ground contact or obstacles.Type: GrantFiled: May 9, 1997Date of Patent: May 30, 2000Assignee: Cybernet Systems CorporationInventors: Gerald P. Roston, Eric Endsley