Abstract: A process for producing a compound of interest from an organic substrate, including the steps of: a) preparing a reaction mixture including at least two bacteria genetically modified to express, in their periplasmic space, at least one enzyme, each of said bacteria expressing an enzyme different from the other bacteria, an enzyme being capable of catalyzing a first reaction using said organic substrate so as to provide a first product, each of the enzymes being capable of catalyzing a reaction using a product or a coproduct obtained by a reaction, so as to provide respectively a product, b) leaving the reaction mixture to react, and c) separating the biomass from the supernatant and extracting therefrom said compound of interest consisting of one of the products.