Patents by Inventor Eric H. Humphries

Eric H. Humphries has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Avian immunoglobulin-producing cell lines
    Patent number: 5258299
    Abstract: The present invention relates to a method of preparing efficiently producing antibody avian cell clones. The method generally involves infecting a B-lymphocyte population with a v-rel gene using transfeThe government may have certain rights in the invention pursuant to grant No. CA 41450 from the National Institutes of Health.
    Type: Grant
    Filed: January 14, 1991
    Date of Patent: November 2, 1993
    Assignee: Board of Regents, The University of Texas System
    Inventor: Eric H. Humphries
  • Chicken-derived immunoglobulin-producing cell lines
    Patent number: 5049502
    Abstract: The present invention involves a method for preparing antibody-producing chicken cell clones. This method comprises a series of steps including initially immunizing a first chicken with a desired antigen. A population of antibody-producing lymphocytes from bursa or spleen of the first chicken is separated.The antibody-producing lymphocyte population is then infected with helper-free reticuloendotheliosis virus REV-T or helper-free reticuloendotheliosis virus REV-T and CSV (preferably REV-T(CSV) and transplanted into a second chicken, the second chicken having been pretreated to remove normal B cells. The transplanted lymphocytes to proliferate in the second chicken, preferably for a period of at least about two weeks.The lymphocytes from spleen, bursa or peripheral blood of the second chicken are isolated and plated out, for example in microtiter plates. Cell clones producing antibody such as IgG or IgM directed against the desired antigen are then selected.
    Type: Grant
    Filed: December 31, 1987
    Date of Patent: September 17, 1991
    Assignee: Board of Regents, The University of Texas System
    Inventor: Eric H. Humphries
  • Avian immunoglobulin-producing cell lines
    Patent number: 5028540
    Abstract: The present invention involves a method for preparing antibody-producing chicken cell clones. This method comprises a series of steps including initially immunizing a first chicken with a desired antigen. A population of antibody-producing lymphocytes from bursa or spleen of the first chicken is separated.The antibody-producing lymphocyte population is then infected with helper-free reticuloendotheliosis virus REV-T or helper-free reticuloendotheliosis virus REV-T and CSV (preferably REV-T(CSV) and transplanted into a second chicken, the second chicken having been pretreated to remove normal B cells. The transplanted lymphocytes to proliferate in the second chicken, preferably for a period of at least about two weeks.The lymphocytes from spleen, bursa or peripheral blood of the second chicken are isolated and plated out, for example in microtiter plates. Cell clones producing antibody such as IgG or IgM directed against the desired antigen are then selected.
    Type: Grant
    Filed: December 21, 1988
    Date of Patent: July 2, 1991
    Assignee: The Board of Regents, The University of Texas System
    Inventor: Eric H. Humphries