Abstract: Multiple component grafts are provided for treatment of tissue defects and comprise two or more components, each of which is a tissue-derived matrix and at least two of which are derived from different types of tissue. For example, a first component may be a matrix derived from cartilage tissue such as cartilage fibers with or without viable cells, cartilage particles with or without viable cells, or combinations of any two or more such cartilage-derived matrices. A second component may be a matrix derived from bone tissue such as mineralized or demineralized cortical bone fibers, viable cancellous bone matrix (e.g., cryopreserved or lyophilized chips, particulates, powder, sheets, putty, flowable fluid, etc.), demineralized or demineralized cancellous bone matrix (chips, particulates, powder, sheets, putty, flowable fluid, etc.), or combinations of any two or more of such bone-derived matrices. Also provided are methods for making and using such multiple component grafts.

Abstract: Preserved tissue samples contain endogenous viable cells and retain or promote biological activity after being stored at temperatures above freezing for extended periods of time (e.g., from 14 days to 3 years). The preserved tissue samples are implanted in or on a subject and, after rehydration, they retain beneficial biological activity, promote beneficial biological activity, or both. The beneficial biological activity comprises promoting one or more of tissue healing, tissue growth, and tissue generation. Methods for preparing the preserved tissue samples include contacting a recovered tissue sample with one or more protectants, followed by lyopreservation. Suitable protectants include sugars, polyphenols, carotenoids, and combinations thereof. Preferred protectants include glucose, fructose, sucrose, trehalose, dextran, EGCG, and combinations thereof. The recovered tissue sample may be any of several possible issue types.

Abstract: Provided herein are compositions containing dehydrated placental tissue particulates, methods of making the compositions and methods for treating various musculoskeletal disorders and other conditions using such compositions, including osteoarthritis (OA), degenerative disc disease, tendonitis, plantar fasciitis, and pain associated therewith.

Abstract: Decellularized muscle matrices are provided for use as implants and grafts to repair, regenerate, supplement, reinforce and replace muscle tissue. The decellularized muscle matrices are derived from muscle tissue having preserved extracellular matrix components, retained muscle-forming potential, and from which immunogenic components have been removed. The decellularized muscle matrices are produced in various physical forms and combinations. Methods for making and using the decellularized muscle matrices are also provided.

Abstract: Provided herein are compositions containing dehydrated placental tissue particulates, methods of making the compositions and methods for treating various musculoskeletal disorders and other conditions using such compositions, including osteoarthritis (OA), degenerative disc disease, tendonitis, plantar fasciitis, and pain associated therewith.

Abstract: The invention is directed toward a cartilage repair assembly comprising a shaped allograft two piece construct with a demineralized cancellous cap and a mineralized cylindrical base member defining a blind bore with a through-going transverse bore intersecting the blind bore. The demineralized cancellous cap has a cylindrical top portion and a smaller diameter cylindrical stem extending away from the top portion which fits into the blind bore of the mineralized base member. The cap stem defines a transverse through-going bore which is aligned with the through-going bore of the base member to receive a cylindrical cortical pin holding the cap within the base member. The shaped structure is dimensioned to fit in a drilled bore in a cartilage defect area so that the assembly engages the side wall of the drilled bore in an interference fit.

Abstract: The invention is directed toward an osteochondral repair assembly comprising a shaped allograft construct comprising an unbalanced barbell-shaped cylindrical cancellous bone primary member formed with a mineralized cylindrical base section having a smaller diameter cylindrical stem leading to a second cylindrical section which is demineralized. A mineralized ring-shaped support member is forced over the compressed demineralized second demineralized the aperture of the ring-shaped member to fit around the stem with one ring surface being adjacent the bottom surface to the second cylindrical section and the opposite ring surface being adjacent the upper surface of the mineralized cylindrical base section.

Abstract: A construct for repairing articular cartilage defects includes a cap member and a base member. The cap member has an upper section and a stem depending from a central region thereof. The upper section includes a peripheral region. The stem includes a cavity. The base member has first and second ends, the first end including an annular recess dimensioned such that the stem is receivable therein. The first end also includes an annular edge positioned laterally outwardly from the annular recess, for abutting and supporting the peripheral region of the upper section when the stem is received in the annular recess. The base member further includes an island which is surrounded by the annular recess and receivable in the cavity of the stem, such that the island is abuttable with and may thereby support the central region of the upper section when the stem is received in the annular recess.

Abstract: Implants comprising a plurality of separate cortical bone units, which have been at least partially demineralized and are osteoinductive, are described herein. The implants can be used in methods for treating bone. Also, disclosed are methods for treating spinal conditions using these implants. The spinal conditions include but are not limited to repairing damage to or defects in the spine, such as fractures in a vertebral body or degeneration of spinal discs.

Abstract: The invention is directed toward a cartilage repair assembly comprising a shaped allograft two piece construct with a demineralized cancellous cap and a mineralized cylindrical base member defining a blind bore with a through-going transverse bore intersecting the blind bore. The demineralized cancellous cap has a cylindrical top portion and a smaller diameter cylindrical stem extending away from the top portion which fits into the blind bore of the mineralized base member. The cap stem defines a transverse through-going bore which is aligned with the through-going bore of the base member to receive a cylindrical cortical pin holding the cap within the base member. The shaped structure is dimensioned to fit in a drilled bore in a cartilage defect area so that the assembly engages the side wall of the drilled bore in an interference fit.

Abstract: Constructs that are at least partially constructed of allograft cancellous bone are disclosed, along with cartilage particles that may be used with the constructs for repairing articular cartilage defects. A multi-piece construct includes a base member, a cap member and at least one pin that secures the cap member to the base member. The base member may be constructed of mineralized cancellous bone, and is used to replace the subchondral bone removed when a surgeon cuts a bore in the area of an adjacent cartilage defect. The base member includes a blind bore and first and second through-going transverse bores in opposite sides of a wall of the base member. The cap member includes an upper section that has a thickness that is similar to that of a patient's surrounding articular cartilage layer and a stem depending from the upper section that is dimensioned to be received in and by the blind bore of the base member.

Abstract: A composition for spinal disc repair comprising a plurality of uniformly shaped demineralized cancellous bone pieces ranging in size from about 2.0 mm to about 4.0 mm loaded into a porous mesh container with the cancellous bone pieces being treated so that they are not osteoinductive and are packed in the porous mesh container under compression forces.

Abstract: A meniscus repair composition for application to a meniscus defect site to promote growth of new tissue at the meniscus defect site is provided. The composition comprises: from about 10 to about 50 percent by weight of allograft meniscus particles having an average particle size of from about 10 ?m to about 500 ?m; a carrier selected from the group consisting of sodium hyaluronate, gelatin, collagen, polyethylene glycol, glycerin, carboxymethylcellulose, dextrose, blood derivatives, aqueous solutions thereof, and mixtures thereof; and a curing agent. The curing agent may be the carrier where the carrier is cross-linkable. When introduced to a defect site in a meniscus and cured, the composition will not flow away from the defect site, and the composition is non-adhering to the defect site after it is cured.

Abstract: A meniscus repair composition for application to a meniscus injury to promote growth of new tissue at the meniscus injury site is provided. The composition comprises: from about 10 to about 50 percent by weight of allograft meniscus particles having an average particle size of from about 10 ?m to about 500 ?m; and a carrier comprising a solid fibrin web matrix. When introduced to a defect site in a meniscus, the composition is non-adhering to the defect site. A method for repairing a meniscus injury comprises administering a meniscus repair composition to the injury site.

Abstract: A sterile implant for treatment of a spinal disc defect comprising an allograft cortical bone demineralized to a Type I collagen having a specific shape which is treated to eliminate osteoinductivity. The implant is lyophilized and compressed into smaller first shape which 20 to 80% from its original shape in at least one dimension and hardened. The implant expanding when hydrated into a second shape having the shape memory of the first shape and expanded in dimensional size from the first compressed shape.

Abstract: Shaped constructs for repair of a defect in a body part or tissue of a subject are discussed herein. More specifically, implants suitable for delivery to a subject, such as a subject having a defect in a body part or body tissue, are discussed. Even more specifically, implants for intervertebral disc repair comprising corticocancellous bone, which can extend into the nucleus pulposus of an intervertebral disc and can be integrally attached to the annulus fibrosus of the disc to keep the implant in position, are described. Also, implants for meniscal repair comprising corticocancellous bone, which can extend from the outer edge of the meniscus to the inner region of the meniscus and can be integrally attached to the meniscal rim to keep the implant in position, are described. Implants for the repair of defects in bone, cartilage, and fibrocartilage are further described.

Abstract: The invention is directed toward a cartilage repair assembly comprising a shaped allograft two piece construct with a demineralized cancellous cap and a mineralized cylindrical base member defining a blind bore with a through going transverse bore intersecting the blind bore. The demineralized cancellous cap has a cylindrical top portion and a smaller diameter cylindrical stem extending away from the top portion which fits into the blind bore of the mineralized base member. The cap stem defines a transverse through going bore which is aligned with the through going bore of the base member to receive a cylindrical cortical pin holding the cap within the base member. The shaped structure is dimensioned to fit in a drilled bore in a cartilage defect area so that the assembly engages the side wall of the drilled bore in an interference fit.

Abstract: Mixtures, such as gels or pastes, comprising freeze-milled cartilage particles and exogenous growth factors are used for repairing chondral defects. Such mixtures may be applied to constructs comprising cancellous bone for implantation at the defect site. Suitable growth factors include variants of FGF-2, particularly variants that include a sole amino acid substitution for asparagine at amino acid 111 of the ?8-?9 loop of the FGF-2 peptide. Such FGF-2 variants are released slowly and continuously at a constant rate from cartilage pastes. In other embodiments, the amino acid substituted for asparigine is glycine. Other variants that may be used include FGF-9 variants having truncated chains and a sole amino acid substitution in the ?8-?9 loop of the FGF-9 peptide either for tryptophan at amino acid 144 or for asparagine at amino acid 143.

Abstract: The invention is directed toward an osteochondral repair assembly comprising a shaped allograft construct comprising an unbalanced barbell-shaped cylindrical cancellous bone primary member formed with a mineralized cylindrical base section having a smaller diameter cylindrical stem leading to a second cylindrical section which is demineralized. A mineralized ring-shaped support member is forced over the compressed demineralized second demineralized the aperture of the ring-shaped member to fit around the stem with one ring surface being adjacent the bottom surface to the second cylindrical section and the opposite ring surface being adjacent the upper surface of the mineralized cylindrical base section.

Abstract: Constructs that are at least partially constructed of allograft cancellous bone are disclosed, along with cartilage particles that may be used with the constructs for repairing articular cartilage defects. A multi-piece construct includes a base member, a cap member and at least one pin that secures the cap member to the base member. The base member may be constructed of mineralized cancellous bone, and is used to replace the subchondral bone removed when a surgeon cuts a bore in the area of an adjacent cartilage defect. The base member includes a blind bore and first and second through-going transverse bores in opposite sides of a wall of the base member. The cap member includes an upper section that has a thickness that is similar to that of a patient's surrounding articular cartilage layer and a stem depending from the upper section that is dimensioned to be received in and by the blind bore of the base member.