Abstract: Systems and methods presented herein provide for elastomeric and flexible cables. In one embodiment, the cables are configured with elastomeric cabling and circuitry. For example, a flexible circuit line (or lines) may be wrapped about an extruded elastomeric substrate (e.g., a polymer). Integrated circuits (e.g., sensors, accelerometers, light emitting diodes, controllers, microprocessors, etc.) may be disposed at various points along the circuit line(s). The cable may then be wrapped with a Polytetrafluoroethylene (PTFE) tape than can be heated to shrink about the cable for protection of the underlying circuitry. Then, the cable may be surrounded with a layer of polymer and extruded to form an elastomeric and flexible cable.

Abstract: The present invention includes a method and kit for cDNA synthesis of a 3?UTR/poly(A) tail junction of cellular RNA comprising: obtaining RNA comprising a 3?UTR/poly(A) junction and a poly(a) tail; combining the RNA with three terminating nucleotides of modified-deoxyGTP, modified-deoxyCTP and modified-deoxyATP, dNTPs, and adaptor sequence-oligo-dT; performing reverse transcription of the RNA with a reverse transcriptase primed with the adaptor sequence-oligo-dT to form terminated cDNA fragments that are stochastically terminated upstream of the 3?UTR/poly(A) junction, but not within the poly(A) tail; isolating the terminated cDNA fragments; chemically ligating a functionalized 5? adaptor to the terminated cDNA; and amplifying the chemically-ligated cDNA into an amplification product, wherein the cDNA is enriched for sequences at the 3?UTR/poly(A) tail junction without fragmentation or enzymatic ligation.

Abstract: Systems and methods presented herein provide for elastomeric and flexible cables. In one embodiment, the cables are configured with elastomeric cabling and circuitry. For example, a flexible circuit line (or lines) may be wrapped about an extruded elastomeric substrate (e.g., a polymer). Integrated circuits (e.g., sensors, accelerometers, light emitting diodes, controllers, microprocessors, etc.) may be disposed at various points along the circuit line(s). The cable may then be wrapped with a Polytetrafluoroethylene (PTFE) tape than can be heated to shrink about the cable for protection of the underlying circuitry. Then, the cable may be surrounded with a layer of polymer and extruded to form an elastomeric and flexible cable.

Abstract: The present invention includes a method and kit for cDNA synthesis of a 3?UTR/poly(A) tail junction of cellular RNA comprising: obtaining RNA comprising a 3?UTR/poly(A) junction and a poly(a) tail; combining the RNA with three terminating nucleotides of modified-deoxyGTP, modified-deoxyCTP and modified-deoxyATP, dNTPs, and adaptor sequence-oligo-dT; performing reverse transcription of the RNA with a reverse transcriptase primed with the adaptor sequence-oligo-dT to form terminated cDNA fragments that are stochastically terminated upstream of the 3?UTR/poly(A) junction, but not within the poly(A) tail; isolating the terminated cDNA fragments; chemically ligating a functionalized 5? adaptor to the terminated cDNA; and amplifying the chemically-ligated cDNA into an amplification product, wherein the cDNA is enriched for sequences at the 3?UTR/poly(A) tail junction without fragmentation or enzymatic ligation.

Abstract: Systems and methods presented herein provide for elastomeric and flexible cables. In one embodiment, the cables are configured with elastomeric cabling and circuitry. For example, a flexible circuit line (or lines) may be wrapped about an extruded elastomeric substrate (e.g., a polymer). Integrated circuits (e.g., sensors, accelerometers, light emitting diodes, controllers, microprocessors, etc.) may be disposed at various points along the circuit line(s). The cable may then be wrapped with a Polytetrafluoroethylene (PTFE) tape than can be heated to shrink about the cable for protection of the underlying circuitry. Then, the cable may be surrounded with a layer of polymer and extruded to form an elastomeric and flexible cable.

Abstract: Systems and methods presented herein provide for elastomeric and flexible cables. One cable includes a first insulator extruded as a tube. The cable also includes an elastomeric conductor comprising conductive particles embedded in a polymer. The elastomeric conductor is extruded with the elastomeric insulator through a conduit of the tube. Other cables include flexible wires extruded with elastomeric tubes. In some embodiments, the cables are configured with stay cords that limit a length of stretching in the cable.

Abstract: Systems and methods presented herein provide for elastomeric and flexible cables. One cable includes a first insulator extruded as a tube. The cable also includes an elastomeric conductor comprising conductive particles embedded in a polymer. The elastomeric conductor is extruded with the elastomeric insulator through a conduit of the tube. Other cables include flexible wires extruded with elastomeric tubes. In some embodiments, the cables are configured with stay cords that limit a length of stretching in the cable.