Abstract: Provided herein is an antibiotic susceptibility and related compositions, methods and systems based on nucleic acid detection based on detected intracellular and extracellular nucleic acid from a same sample, which allows determination of antibiotic susceptibility of microorganisms as well as the diagnosis and/or treatment of related infections in individuals.

Abstract: Implementations of this disclosure are directed to systems, devices and methods for implementing a cache data management system. Webserver computers receive cache data requests for data stored at a computer cluster comprising a plurality of master cache data server computers that do not have corresponding slave cache data server computers to store reserve cache data. Proxy computers in communication with the plurality of webserver computers and the computer cluster route the cache data requests from the webserver computers to the computer cluster. Each proxy computer includes a sentinel module to monitor a health of the computer cluster by detecting failures of master cache data server computers and a trask monitor agent to manage the computer cluster.

Abstract: Implementations of this disclosure are directed to systems, devices and methods for implementing a cache data management system. Webserver computers receive cache data requests for data stored at a computer cluster comprising a plurality of master cache data server computers that do not have corresponding slave cache data server computers to store reserve cache data. Proxy computers in communication with the plurality of webserver computers and the computer cluster route the cache data requests from the webserver computers to the computer cluster. Each proxy computer includes a sentinel module to monitor a health of the computer cluster by detecting failures of master cache data server computers and a trask monitor agent to manage the computer cluster.

Abstract: Implementations of this disclosure are directed to systems, devices and methods for implementing a cache data management system. Webserver computers receive cache data requests for data stored at a computer cluster comprising a plurality of master cache data server computers that do not have corresponding slave cache data server computers to store reserve cache data. Proxy computers in communication with the plurality of webserver computers and the computer cluster route the cache data requests from the webserver computers to the computer cluster. Each proxy computer includes a sentinel module to monitor a health of the computer cluster by detecting failures of master cache data server computers and a trask monitor agent to manage the computer cluster.

Abstract: Implementations of this disclosure are directed to systems, devices and methods for implementing a cache data management system. Webserver computers receive cache data requests for data stored at a computer cluster comprising a plurality of master cache data server computers that do not have corresponding slave cache data server computers to store reserve cache data. Proxy computers in communication with the plurality of webserver computers and the computer cluster route the cache data requests from the webserver computers to the computer cluster. Each proxy computer includes a sentinel module to monitor a health of the computer cluster by detecting failures of master cache data server computers and a trask monitor agent to manage the computer cluster.

Abstract: Embodiments provided herein generally relate to methods and materials for ultrasound-mediated introduction of exogenous substances into microorganisms. Some embodiments relate to methods of introducing an exogenous material into a microorganism such as an algae. The methods can include, for example, providing a microorganism (e.g., algae); providing a population of bubbles that comprise one or more nucleic acid molecules and/or one or more polypeptide or one or more protein molecules; contacting the population of bubbles with the algae; applying ultrasound to the bubbles and algae with sufficient energy to cavitate one or more of bubbles comprising the one or more nucleic acid molecules in proximity to the algae; and maintaining the algae and the one or more burst bubbles comprising the one or more nucleic acid molecules in contact for a period of time sufficient to permit entry of at least one nucleic acid molecule into the algae.

Abstract: Embodiments provided herein generally relate to methods and materials for ultrasound-mediated introduction of exogenous substances into microorganisms. Some embodiments relate to methods of introducing an exogenous material into a microorganism such as an algae. The methods can include, for example, providing a microorganism (e.g., algae); providing a population of bubbles that comprise one or more nucleic acid molecules and/or one or more polypeptide or one or more protein molecules; contacting the population of bubbles with the algae; applying ultrasound to the bubbles and algae with sufficient energy to cavitate one or more of bubbles comprising the one or more nucleic acid molecules in proximity to the algae; and maintaining the algae and the one or more burst bubbles comprising the one or more nucleic acid molecules in contact for a period of time sufficient to permit entry of at least one nucleic acid molecule into the algae.