Abstract: The application generally relates to bioproduction of molecules of interest in microorganisms, more particularly in microalgae. In particular, the application relates to methods for increasing triacylglycerol production in micro-organisms, in particular in microalgae, using recombinant micro-organisms which have been genetically engineered to express or overexpress a mutant of a bubblegum-type acyl-CoA synthase, and uses thereof.

Abstract: The application generally relates to chloroplast targeting of nuclear-encoded proteins of interest in microalgae. Provided herein are expression cassettes comprising a nucleotide sequence encoding a chloroplast targeting peptide operably linked to a nucleotide sequence encoding a protein of interest, wherein said chloroplast targeting peptide comprises the bipartite targeting sequence of the phosphoribulokinase of Nannochloropsis gaditana (NgPRK BTS). The invention further provides vectors comprising the expression cassettes, and microalgae having stably incorporated or transiently expressed into their nuclear genomes an expression cassette described above. Methods are also provided for the production of a protein of interest in the chloroplast of a microalga, as well as methods for modulating chloroplast pathways.

Abstract: The disclosure pertains to a method for the production of triacylglycerides (TAGs or Triacylglyerols) and fatty acids by the recombinant expression of a ?11 fatty acid desaturase in protists.

Abstract: The invention relates to a method for triggering triacylglycerols (TAG) accumulation in microalgae comprising the step of contacting a source of exogenous nitroxide (NO) with said microalgae in their growth medium.

Abstract: The invention relates to a method for accumulating triacylglycerols in microalgae by inhibiting the sterol metabolism, by incubating the microalgae with an inhibitor of sterol metabolism. The invention also relates to a method for producing fatty acids, biofuels, pharmaceutical or cosmetic compositions, and also food supplements, comprising a triacylglycerols accumulation step in microalgae according to the invention. Finally, the invention concerns the use of an inhibitor of sterol metabolism to accumulate triglycerides in microorganisms, and preferably microalgae.

Abstract: The present technology relates to a microalga that is genetically modified by inhibiting the activity of the enzyme ?0-elongase (?0-ELO), which uses palmitic acid, a saturated fatty acid, as substrate and is associated with the production of monogalactosyldiacylglycerol (MGDG). The present technology also relates to a process for culturing the microalga for increased production of triacylglycerols (TAGs) and recovery of the TAGs.

Abstract: The application generally relates to bioproduction of molecules of interest in micro-organisms, more particularly in microalgae. In particular, the application relates to methods for increasing triacylglycerol production in micro-organisms, in particular in microalgae, using recombinant micro-organisms which have been genetically engineered to produce or overproduce nitric oxide (NO) and uses thereof.

Abstract: The application generally relates to chloroplast targeting of nuclear-encoded proteins of interest in microalgae. Provided herein are expression cassettes comprising a nucleotide sequence encoding a chloroplast targeting peptide operably linked to a nucleotide sequence encoding a protein of interest, wherein said chloroplast targeting peptide comprises the bipartite targeting sequence of the phosphoribulokinase of Nannochloropsis gaditana (NgPRK BTS). The invention further provides vectors comprising the expression cassettes, and microalgae having stably incorporated or transiently expressed into their nuclear genomes an expression cassette described above. Methods are also provided for the production of a protein of interest in the chloroplast of a microalga, as well as methods for modulating chloroplast pathways.

Abstract: A method and a device for determining a concentration of lipids in a microorganism such as a micro-alga by illuminating a sample containing microorganisms and acquiring a total diffraction pattern of the sample is acquired, the total diffraction pattern including a plurality of unit diffraction pattern each associated with a microorganism.

Abstract: The present invention relates to a genetically engineered algae strain in which the expression of the CGI-58 gene or homologous gene thereof is silenced. The present invention further relates to a method of triacylglycerol accumulation using said genetically engineered diatom and/or diatom strain.

Abstract: The invention relates to a method for triggering triacylglycerols (TAG) accumulation in microalgae comprising the step of contacting a source of exogenous nitroxide (NO) with said microalgae in their growth medium.

Abstract: The invention relates to a method for accumulating triacylglycerols in microalgae by inhibiting the sterol metabolism, N by incubating the microalgae with an inhibitor of sterol metabolism. The invention also relates to a method for producing fatty acids, biofuels, pharmaceutical or cosmetic compositions, and also food supplements, comprising a triacylglycerols accumulation step in microalgae according to the invention. Finally, the invention concerns the use of an inhibitor of sterol metabolism to accumulate triglycerides in microorganisms, and preferably microalgae.

Abstract: The invention relates to the use of compounds having a monogalactosyldiacylglycerol (MGDG) synthase inhibitory activity as herbicide or algaecide, and to herbicide and algaecide compositions containing at least one of these compounds.

Abstract: Novel polyspirane compounds used in the treatment of diseases involving parasites that belong to the phylum of apicomplexae, and a method for preparing the same.

Abstract: Novel polyspirane compounds used in the treatment of diseases involving parasites that belong to the phylum of apicomplexae, and a method for preparing the same.

Abstract: The Invention relates to compounds having an antiparasitic activity, and to their use as a drug, in particular as a drug for the prevention and/or treatment of parasitic diseases caused by apicomplexans. The invention also relates to pharmaceutical compositions containing those compounds.

Abstract: The invention relates to the use of compounds having a monogalactosyldiacylglycerol (MGDG) synthase inhibitory activity as herbicide or algaecide, and to herbicide and algaecide compositions containing at least one of these compounds.

Abstract: The invention relates to a digalactolipidic antigen exposed on the surface of apicomplex parasites, in the form of a vegetable-type digalactoglycerolipid, and adapted for inducing the production of specific antibodies capable of inhibiting the proliferation and/or the invasive properties of said parasites; the invention also relates to a derived antibody or functional antibody fragment, and to their diagnostic, immunotherapeutic and vaccine applications in human beings or animals.

Abstract: The invention concerns membrane fractions of cells containing a recombinant MGDG synthase and enriched with 1,2-sn-diacylglycerol, their preparation method, their use for screening molecules inducing MGDG synthase activity and a method for screening molecules inducing MGDG synthase activity using said membrane fractions.

Abstract: The invention concerns a method for screening and selecting parasiticides (apicomplex phyllum parasites) and/or herbicides and the uses thereof. Said method consists in incubating a substance to be tested with a MGDG synthase and measuring the specific enzymatic activity, after said incubation. The invention also concerns the use of MGDG synthase or a plant isolated plastid membrane for selecting or screening the products inhibiting the activity of the MGDG synthase, capable of being used as active principles against apicomplex parasites and/or of being used as herbicides.