Patents by Inventor Eric Marechal

Eric Marechal has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10590444
    Abstract: The application generally relates to bioproduction of molecules of interest in micro-organisms, more particularly in microalgae. In particular, the application relates to methods for increasing triacylglycerol production in micro-organisms, in particular in microalgae, using recombinant micro-organisms which have been genetically engineered to produce or overproduce nitric oxide (NO) and uses thereof.
    Type: Grant
    Filed: January 27, 2017
    Date of Patent: March 17, 2020
    Assignee: TOTAL RAFFINAGE CHIMIE
    Inventors: Eric Marechal, Lina Dolch
  • Publication number: 20190352346
    Abstract: The application generally relates to chloroplast targeting of nuclear-encoded proteins of interest in microalgae. Provided herein are expression cassettes comprising a nucleotide sequence encoding a chloroplast targeting peptide operably linked to a nucleotide sequence encoding a protein of interest, wherein said chloroplast targeting peptide comprises the bipartite targeting sequence of the phosphoribulokinase of Nannochloropsis gaditana (NgPRK BTS). The invention further provides vectors comprising the expression cassettes, and microalgae having stably incorporated or transiently expressed into their nuclear genomes an expression cassette described above. Methods are also provided for the production of a protein of interest in the chloroplast of a microalga, as well as methods for modulating chloroplast pathways.
    Type: Application
    Filed: December 5, 2017
    Publication date: November 21, 2019
    Inventors: Leonardo Magneschi, Eric Marechal, Giovanni Finazzi, Mariette Bedhomme, Séverine Collin, Frédéric Laeuffer, Laurent Fourage
  • Publication number: 20190264183
    Abstract: The invention relates to a microalga which is genetically modified by inhibiting the ?0 elongase (?0-ELO) enzyme activity, using palmitic acid, a saturated fatty acid, as a substrate, and which is associated with the production of mono-galactosyl diacylglycerol (MGDG). The invention also relates to a method for cultivating said microalga for increased triacylglycerol (TAG) production and harvesting of said TAG.
    Type: Application
    Filed: June 27, 2017
    Publication date: August 29, 2019
    Inventors: Lina Juana DOLCH, Camille RAK, Fabrice REBEILLE, Juliette JOUHET, Marina LETERRIER, Eric MARECHAL
  • Publication number: 20190024101
    Abstract: The present application relates to an expression system for multigene overexpression in microalgae, which expression system comprises at least two nucleic acid expression cassettes, wherein each expression cassette comprises a promoter operably linked to three or more transgenes connected to one another by at least one sequence encoding a 2A peptide (i.e. a multicistronic construct). Also disclosed herein are vector systems comprising said expression systems, host cells transformed with said expression systems or comprising said vector systems, methods for producing these host cells, as well as their use for biosynthesis.
    Type: Application
    Filed: January 27, 2017
    Publication date: January 24, 2019
    Inventors: Séverine COLLIN, Laurent FOURAGE, Fédéric LAEUFFER, Mariette BEDHOMME, Leonardo MAGNESCHI, Giovanni FINAZZI, Eric MARECHAL
  • Publication number: 20190017082
    Abstract: The application generally relates to bioproduction of molecules of interest in micro- organisms, more particularly in microalgae. In particular, the application relates to methods for increasing triacylglycerol production in micro-organisms, in particular in microalgae, using recombinant micro-organisms which have been genetically engineered to produce or overproduce nitric oxide (NO) and uses thereof.
    Type: Application
    Filed: January 27, 2017
    Publication date: January 17, 2019
    Inventors: Eric MARECHAL, Lina DOLCH
  • Patent number: 10073022
    Abstract: A method and a device for determining a concentration of lipids in a microorganism such as a micro-alga by illuminating a sample containing microorganisms and acquiring a total diffraction pattern of the sample is acquired, the total diffraction pattern including a plurality of unit diffraction pattern each associated with a microorganism.
    Type: Grant
    Filed: February 23, 2015
    Date of Patent: September 11, 2018
    Assignee: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
    Inventors: Jerome Boutet, Cedric Allier, Giovanni Finazzi, Eric Marechal
  • Patent number: 9938545
    Abstract: The present invention relates to a genetically engineered algae strain in which the expression of the CGI-58 gene or homologous gene thereof is silenced. The present invention further relates to a method of triacylglycerol accumulation using said genetically engineered diatom and/or diatom strain.
    Type: Grant
    Filed: July 21, 2014
    Date of Patent: April 10, 2018
    Assignees: FERMENTALG, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE(C.N.R.S), COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
    Inventors: Pierre Calleja, Marina Leterrier, Eric Marechal
  • Publication number: 20170218412
    Abstract: The invention relates to a method for triggering triacylglycerols (TAG) accumulation in microalgae comprising the step of contacting a source of exogenous nitroxide (NO) with said microalgae in their growth medium.
    Type: Application
    Filed: January 27, 2017
    Publication date: August 3, 2017
    Inventors: Eric Marechal, Lina Juana Dolch
  • Publication number: 20170010200
    Abstract: The invention relates to a method for determining a concentration of lipids in a microorganism, in particular a micro-alga, wherein: a sample containing microorganisms is illuminated (101); and a total diffraction pattern (12) of the sample is acquired (102), the total diffraction pattern comprising a plurality of unit diffraction patterns (131; 14A; 14B) each associated with a microorganism. According to the invention, a value of a numerical indicator (In) representative of a dispersion of the light intensity in a zone of interest (13) of the total diffraction pattern is determined (104), and a concentration of lipids (Cx) in the microorganisms is deduced therefrom. The invention also relates to a device for implementing such a method.
    Type: Application
    Filed: February 23, 2015
    Publication date: January 12, 2017
    Applicant: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
    Inventors: Jerome BOUTET, Cedric ALLIER, Giovanni FINAZZI, Eric MARECHAL
  • Publication number: 20160348138
    Abstract: The invention relates to a method for accumulating triacylglycerols in microalgae by inhibiting the sterol metabolism, N by incubating the microalgae with an inhibitor of sterol metabolism. The invention also relates to a method for producing fatty acids, biofuels, pharmaceutical or cosmetic compositions, and also food supplements, comprising a triacylglycerols accumulation step in microalgae according to the invention. Finally, the invention concerns the use of an inhibitor of sterol metabolism to accumulate triglycerides in microorganisms, and preferably microalgae.
    Type: Application
    Filed: January 27, 2015
    Publication date: December 1, 2016
    Inventors: Melissa Conte, Lina-Juana Dolch, Coline Mei, Caroline Barette, Dimitris Petroutsos, Denis Falconet, Juliette Jouhet, Fabrice Rebeille, Jean-Christophe Cintrat, Eric Marechal
  • Publication number: 20160160247
    Abstract: The present invention relates to a genetically engineered algae strain in which the expression of the CGI-58 gene or homologous gene thereof is silenced. The present invention further relates to a method of triacylglycerol accumulation using said genetically engineered diatom and/or diatom strain.
    Type: Application
    Filed: July 21, 2014
    Publication date: June 9, 2016
    Inventors: Pierre CALLEJA, Marina LETERRIER, Eric MARECHAL
  • Patent number: 8637496
    Abstract: The invention relates to the use of compounds having a monogalactosyldiacylglycerol (MGDG) synthase inhibitory activity as herbicide or algaecide, and to herbicide and algaecide compositions containing at least one of these compounds.
    Type: Grant
    Filed: June 2, 2008
    Date of Patent: January 28, 2014
    Assignees: Commissariat a l'Energie Atomique, Centre National de la Recherche Scientifique
    Inventors: Anne-Laure Bonneau, Cyrille Botte, Michael Deligny, Nadia Saidani, Hélène Hardre, Bernard Rousseau, Roman Lopez, Eric Marechal
  • Patent number: 8389569
    Abstract: Novel polyspirane compounds used in the treatment of diseases involving parasites that belong to the phylum of apicomplexae, and a method for preparing the same.
    Type: Grant
    Filed: October 2, 2008
    Date of Patent: March 5, 2013
    Assignee: Universite Joseph Fourier
    Inventors: Yung-Sing Wong, Marine Aimée Peuchmaur, Eric Marechal, Cyrille Botte, Henri Joseph Vial, Nadia Saidani
  • Publication number: 20100298422
    Abstract: Novel polyspirane compounds used in the treatment of diseases involving parasites that belong to the phylum of apicomplexae, and a method for preparing the same.
    Type: Application
    Filed: October 2, 2008
    Publication date: November 25, 2010
    Applicants: UNIVERSITE JOSEPH FOURIER, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
    Inventors: Yung-Sing Wong, Marine Aimee Peuchmaur, Eric Marechal, Cyrille Botte, Henri Joseph Vial, Nadia Saidani
  • Publication number: 20100292214
    Abstract: The Invention relates to compounds having an antiparasitic activity, and to their use as a drug, in particular as a drug for the prevention and/or treatment of parasitic diseases caused by apicomplexans. The invention also relates to pharmaceutical compositions containing those compounds.
    Type: Application
    Filed: June 2, 2008
    Publication date: November 18, 2010
    Applicants: commissariat a l'Energie Atomique, Centre National De La Recherche Scientifique
    Inventors: Michael Deligny, Nadia Saidani, Anne-Laure Bonneau, Cyrille Botte, Helene Hardre, Bernard Rousseau, Henri Vial, Corinne Mercier, Roman Lopez, Eric Marechal
  • Publication number: 20100273653
    Abstract: The invention relates to the use of compounds having a monogalactosyldiacylglycerol (MGDG) synthase inhibitory activity as herbicide or algaecide, and to herbicide and algaecide compositions containing at least one of these compounds.
    Type: Application
    Filed: June 2, 2008
    Publication date: October 28, 2010
    Applicants: COMMISSARIAT A L'ENERGIE ATOMIQUE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
    Inventors: Anne-Laure Bonneau, Cyrille Botte, Michael Deligny, Nadia Saidani, Helene Hardre, Bernard Rousseau, Roman Lopez, Eric Marechal
  • Publication number: 20100040615
    Abstract: The invention relates to a digalactolipidic antigen exposed on the surface of apicomplex parasites, in the form of a vegetable-type digalactoglycerolipid, and adapted for inducing the production of specific antibodies capable of inhibiting the proliferation and/or the invasive properties of said parasites; the invention also relates to a derived antibody or functional antibody fragment, and to their diagnostic, immunotherapeutic and vaccine applications in human beings or animals.
    Type: Application
    Filed: February 7, 2008
    Publication date: February 18, 2010
    Applicants: COMMISSARIAT A L'ENERGIE ATOMIQUE, CENTRE NATIONAL DE LA RECHERCHE SEIENTIFIQUE
    Inventors: Cyrille Botte, Nadia Saidani, Maryse Block, Jean-Francois Dubremetz, Henri Vial, Marie-France Cesbron-Delauw, Corinne Mercier, Eric Marechal
  • Patent number: 7435583
    Abstract: The invention concerns membrane fractions of cells containing a recombinant MGDG synthase and enriched with 1,2-sn-diacylglycerol, their preparation method, their use for screening molecules inducing MGDG synthase activity and a method for screening molecules inducing MGDG synthase activity using said membrane fractions.
    Type: Grant
    Filed: October 26, 2001
    Date of Patent: October 14, 2008
    Assignees: Commissariat a l'Energie Atomique, Centre National de la Recherche Scientifique
    Inventors: Eric Marechal, Stéphane Miras, Jacques Joyard
  • Patent number: 6924092
    Abstract: The invention concerns a method for screening and selecting parasiticides (apicomplex phyllum parasites) and/or herbicides and the uses thereof. Said method consists in incubating a substance to be tested with a MGDG synthase and measuring the specific enzymatic activity, after said incubation. The invention also concerns the use of MGDG synthase or a plant isolated plastid membrane for selecting or screening the products inhibiting the activity of the MGDG synthase, capable of being used as active principles against apicomplex parasites and/or of being used as herbicides.
    Type: Grant
    Filed: March 17, 2000
    Date of Patent: August 2, 2005
    Assignees: Commissariat a L'Energie Atomique, Centre National de la Recherche Scientifique
    Inventors: Eric Marechal, Maryse Block, Jacques Joyard, Roland Douce
  • Publication number: 20040013739
    Abstract: The invention concerns membrane fractions of cells containing a recombinant MGDG synthase and enriched with 1,2-sn-diacylglycerol, their preparation method, their use for screening molecules inducing MGDG synthase activity and a method for screening molecules inducing MGDG synthase activity using said membrane fractions.
    Type: Application
    Filed: April 30, 2003
    Publication date: January 22, 2004
    Inventors: Eric Marechal, Stephane Miras, Jacques Joyard