Abstract: A chimeric helper nucleic acid molecule contains polynucleotides encoding helper functions from three distinct viruses for producing recombinant adeno-associated virus (rAAV). The molecule includes adenoviral elements with an E2A gene, an E4 gene, and a VA-RNA polynucleotide; a herpes simplex virus (HSV) polynucleotide encoding a UL12 protein or ICP8 protein; and a human bocavirus (HBoV) polynucleotide encoding a NS2 protein or NP1 protein. These polynucleotides are arranged as individual expression cassettes under non-endogenous promoter control. The chimeric helper molecule, when used in combination with an AAV transfer plasmid and Rep/Cap plasmid, provides enhanced production of infectious rAAV particles compared to conventional helper plasmids containing only adenoviral elements. The molecule enables efficient production of multiple AAV serotypes across different cell lines and transfection conditions.

Abstract: Ornamental articles and devices such as, but not limited to, charms, flags, animated characters, flashlights, magnifying glasses, and gas pump speed pay devices clipped to or otherwise attached to a writing instrument, such as a pen. In a preferred embodiment, the ornamental articles and other devices clip to the top of the writing instrument. In a further embodiment, the ornamental articles and devices attach to the pocket clip of a writing instrument and are visibly displayed when the writing instrument is carried in a pocket of an article of clothing.

Abstract: A device for installation in an automobile including a passenger car, minivan or bus (e.g., a school bus) indicates the position of each window and emergency exit of the vehicle and sounds an alarm to indicate to the driver of the vehicle that one or more of the windows and/or emergency exits is open. The alarm may be a combination of a visual lighted display on the driver console and/or an audible alarm indicator. The visual indicator may further indicate the operative position of any particular window or emergency door opening between the closed position, slight open, half open and fully open positions. The device alerts the driver of the vehicle to correct the window position of each window and emergency exit of the vehicle for purposes of safety as well as to prevent robbery and/or damage to the vehicle (e.g., from rain, sprinklers, etc.) when leaving the vehicle unoccupied. The device may also alert the driver to crack the windows partially open when leaving a pet in the vehicle for a short time.