Abstract: Provided are compositions comprising a target cell binding moiety conjugated with a nucleic acid polymer, the nucleic acid polymer characterized by a predicted melting temperature range (of secondary structures thereof) and/or a predicted free energy range (of self-hybridization). The compositions may be included in kits or systems, together with one or more complementary oligonucleotides, competitor oligonucleotides, anti-competitor oligonucleotides or the like. The compositions, kits, and systems may be used in methods of stimulating cells, quenching the stimulation of cells, modulating the stimulation of cells, or tagging (stimulated or unstimulated) cells to be analyzed.

Abstract: A transfection reagent composition comprising: 30-60 MOL. % of a cationic lipid, or a pharmaceutical acceptable salt thereof; 10-60 MOL % of a structural lipid; 10-20 MOL % of a triglyceride; and 0.1 to about 10 MOL. % of a stabilizing agent, is provided. The transfection agent is effective in transfecting cells, particularly neurons, with siRNA, mRNA and plasmid nucleic acid, Sand maintaining viability of the cells as well as activity of the delivered nucleic acid.

Abstract: A transfection reagent composition comprising: 30-60 MOL % of an cationic lipid, or pharmaceutical acceptable salt thereof; 10-60 MOL % structural lipid; a sterol and 0.1 to about 10 MOL % of a stabilizing agent is provided. The reagent is particularly adapted for neuron and related cell types. A method of manufacturing LNP including nucleic acid for selective uptake into either neurons or astrocytes or neural progenitor cells is also provided.

Abstract: A transfection reagent composition comprising: 30-60 MOL. % of a cationic lipid, or a pharmaceutical acceptable salt thereof; 10-60 MOL % of a structural lipid; 10-20 MOL % of a triglyceride; and 0.1 to about 10 MOL. % of a stabilizing agent, is provided. The transfection agent is effective in transfecting cells, particularly neurons, with siRNA, mRNA and plasmid nucleic acid, Sand maintaining viability of the cells as well as activity of the delivered nucleic acid.

Abstract: Apparatuses and methods for cutting a facestock on a liner. An anvil roller receives facestock on liner. A cutting roller, under a force, cuts facestock between the cutting roller and the anvil roller. At least one support roller supports the cutting roller and, optionally, the anvil roller, thereby distributing the force into two or more portions and/or subportions, in two or more directions. Apparatuses, methods and kits for preparing a cutting surface of a cutting roller in a facestock cutting apparatus, or for extending the usefulness of a cutting roller. An equalizer roller is provided in a position adjacent to the cutting roller. The equalizer roller has an equalizing surface having a hardness at least as hard as a hardness of the cutting surface. The cutting roller is rotated against the equalizer roller such that the cutting surface is equalized by the equalizing surface.

Abstract: Apparatuses and methods for cutting a facestock on a liner. An anvil roller receives facestock on liner. A cutting roller, under a force, cuts facestock between the cutting roller and the anvil roller. At least one support roller supports the cutting roller and, optionally, the anvil roller, thereby distributing the force into two or more portions and/or subportions, in two or more directions. Apparatuses, methods and kits for preparing a cutting surface of a cutting roller in a facestock cutting apparatus, or for extending the usefulness of a cutting roller. An equalizer roller is provided in a position adjacent to the cutting roller. The equalizer roller has an equalizing surface having a hardness at least as hard as a hardness of the cutting surface. The cutting roller is rotated against the equalizer roller such that the cutting surface is equalized by the equalizing surface.

Abstract: Apparatuses and methods for cutting a facestock on a liner. An anvil roller receives facestock on liner. A cutting roller, under a force, cuts facestock between the cutting roller and the anvil roller. At least one support roller supports the cutting roller and, optionally, the anvil roller, thereby distributing the force into two or more portions and/or subportions, in two or more directions. Apparatuses, methods and kits for preparing a cutting surface of a cutting roller in a facestock cutting apparatus, or for extending the usefulness of a cutting roller. An equalizer roller is provided in a position adjacent to the cutting roller. The equalizer roller has an equalizing surface having a hardness at least as hard as a hardness of the cutting surface. The cutting roller is rotated against the equalizer roller such that the cutting surface is equalized by the equalizing surface.

Abstract: Apparatuses and methods for cutting a facestock on a liner. An anvil roller receives facestock on liner. A cutting roller, under a force, cuts facestock between the cutting roller and the anvil roller. At least one support roller supports the cutting roller and, optionally, the anvil roller, thereby distributing the force into two or more portions and/or subportions, in two or more directions. Apparatuses, methods and kits for preparing a cutting surface of a cutting roller in a facestock cutting apparatus, or for extending the usefulness of a cutting roller. An equalizer roller is provided in a position adjacent to the cutting roller. The equalizer roller has an equalizing surface having a hardness at least as hard as a hardness of the cutting surface. The cutting roller is rotated against the equalizer roller such that the cutting surface is equalized by the equalizing surface.