Abstract: The present invention is related to sustainable fermentation processes with increased efficiency and less environmental impact. Particularly, the present invention is related to a process wherein in one fermentation process two or more fermentation products can be produced and isolated, i.e. a “primary” fermentation product and a “secondary” fermentation product, particularly wherein one is a water soluble organic compound and one is a fat-soluble organic compound particularly a fat-soluble vitamin, preferably vitamin K2.

Abstract: A belt step conveyor system includes a moving belt gripper mounted on a linear movement system to convey the conveyor belt rotating about a plurality of pulleys in successive distance movements while the moving belt gripper engages the conveying belt and the moving belt gripper is moved from a home position to an end position by linear movement system. The conveyor belt is stagnant by engaging a stagnating belt gripper while the moving belt gripper moves back to its home position, else the stagnating belt gripper releases the conveyor belt.

Abstract: A belt step conveyor system includes a moving belt gripper mounted on a linear movement system to convey the conveyor belt rotating about a plurality of pulleys in successive distance movements while the moving belt gripper engages the conveying belt and the moving belt gripper is moved from a home position to an end position by linear movement system. The conveyor belt is stagnant by engaging a stagnating belt gripper while the moving belt gripper moves back to its home position, else the stagnating belt gripper releases the conveyor belt.

Abstract: We describe a method for purifying chymosin comprising providing an aqueous liquid sample containing chymosin and a separation medium comprising a base matrix and a plurality of firmly attached ligands that are capable of binding to chymosin, contacting the matrix with the sample under conditions permitting binding of chymosin to the matrix, and desorbing chymosin from the matrix. The characterizing feature is that the matrix is hydrophilic and that the ligands in the plurality of ligands are hydrocarbon groups in which all carbon atoms are sp3-hybridised, possibly with an ether oxygen or a thioether sulphur inserted between two carbon atoms at one or more positions in at least one of the hydrocarbon groups, and possibly a hydroxy group replacing a hydrogen atom at one or more positions in at least one of the hydrocarbon groups.

Abstract: A method for purifying chymosin comprising the steps of: providing an aqueous liquid sample containing chymosin and a separation medium comprising a base matrix and a plurality of firmly attached ligands that are capable of binding to chymosin; contacting the matrix with the sample under conditions permitting binding of chymosin to the matrix; and desorbing chymosin from the matrix. The characterizing feature is that the matrix is hydrophilic and that the ligands in the plurality of ligands are hydrocarbon groups in which all carbon atoms are sp3-hybridised, possibly with an ether oxygen or a thioether sulphur inserted between two carbon atoms at one or more positions in at least one of the hydrocarbon groups, and possibly a hydroxy group replacing a hydrogen atom at one or more positions in at least one of the hydrocarbon groups.