Abstract: Disclosed herein are screening tools for fetal/maternal wellness, such as biomarkers for assessing preeclampsia. More specifically, methods are disclosed for assessing the risk of preeclampsia in a subject, the methods including obtaining a first serum sample from the subject, determining a level of glycosylated fibronectin (GlyFn) in the first serum sample, and comparing the determined level of GlyFn with a control value, wherein an elevation in the determined level of GlyFn in the first serum sample relative to the control value indicates that the subject is at increased risk of preeclampsia. Also disclosed are methods of determining the risk of preeclampsia in a subject during a first, second, or third trimester, methods of assessing severity and progression of preeclampsia and complications of a risk of low birth weight or HELLP syndrome.

Abstract: Disclosed herein are screening tools for fetal/maternal wellness, such as biomarkers for assessing preeclampsia. More specifically, methods are disclosed for assessing the risk of preeclampsia in a subject, the methods including obtaining a first serum sample from the subject, determining a level of glycosylated fibronectin (GlyFn) in the first serum sample, and comparing the determined level of GlyFn with a control value, wherein an elevation in the determined level of GlyFn in the first serum sample relative to the control value indicates that the subject is at increased risk of preeclampsia. Also disclosed are methods of determining the risk of preeclampsia in a subject during a first, second, or third trimester, methods of assessing severity and progression of preeclampsia and complications of a risk of low birth weight or HELLP syndrome.

Abstract: Disclosed herein are methods and tests for diagnosing and/or monitoring a metabolic condition such as diabetes in a subject, wherein the methods and tests measure salivary glycoproteins. Some of the methods are based on the oxidation of glycoproteins in a sample from the subject, such as saliva or urine, for example using sodium metaperiodate, and then detecting the aldehydes generated during oxidation using a chemical detection method. Also disclosed are kits and lateral flow devices for detecting glycoproteins in a saliva sample.

Abstract: Disclosed herein are screening tools for fetal/maternal wellness, such as biomarkers for assessing preeclampsia. More specifically, methods are disclosed for assessing the risk of preeclampsia in a subject, the methods including obtaining a first serum sample from the subject, determining a level of glycosylated fibronectin (GlyFn) in the first serum sample, and comparing the determined level of GlyFn with a control value, wherein an elevation in the determined level of GlyFn in the first serum sample relative to the control value indicates that the subject is at increased risk of preeclampsia. Also disclosed are methods of determining the risk of preeclampsia in a subject during a first, second, or third trimester, methods of assessing severity and progression of preeclampsia and complications of a risk of low birth weight or HELLP syndrome.

Abstract: Disclosed herein are methods and tests for diagnosing and/or monitoring a metabolic condition such as diabetes in a subject, wherein the methods and tests measure salivary glycoproteins. Some of the methods are based on the oxidation of glycoproteins in a sample from the subject, such as saliva or urine, for example using sodium metaperiodate, and then detecting the aldehydes generated during oxidation using a chemical detection method. Also disclosed are kits and lateral flow devices for detecting glycoproteins in a saliva sample.

Abstract: A saliva enhancement reagent for use in an ELISA wherein a horseradish peroxidase (HRP) enzyme conjugate system is disclosed.

Abstract: An immunoassay method and apparatus for diagnosing FeLV-induced, persistent viremia is provided comprising combining a feline saliva sample conveniently obtained with a specially designed probe, with anti-p27 antibodies and detecting complex formation as indicative of the viremia. Preferably, the antibodies are bound to the probe for complex formation.

Abstract: The saliva test for feline leukemia virus (FeLV) employs a probe having an immunochemically sensitive member for collecting saliva from the oral cavity of a cat and employs ELISA reagents for the incubation of the probe and the development of color reactions to indicate the presence or absence of FeLV within the saliva sample collected onto the probe.

Abstract: The performance of immunoassays for the analysis of serum analytes can be significantly improved by the pretreatment of the sample. Analyte in serum samples is often complexed with serum antibody. Such analyte-antibody complexes can mask the analyte and interfere with analyte specific binding steps of many immunoassays. The serum pretreatment method employs pH dependent chaotropes to dissociate the analyte-antibody complexes in the serum. At low pH, the complexes become dissociated and the antibody becomes denatured. After the dissociation and denaturation of the serum antibody, the serum sample and the chaotrope, contained therein, are then neutralized. Since the serum antibody has been denatured, it does not re-associate with the analyte upon neutralization. After the neutralization step, serum sample can then be analyzed by an immunoassay, without interference from serum antibody. A serum pretreatment kit is essential to the employment of the serum pretreatment method.