Patents by Inventor Eric Takeshi HARADA
Eric Takeshi HARADA has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230416813Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.Type: ApplicationFiled: June 19, 2023Publication date: December 28, 2023Applicant: Roche Sequencing Solutions, Inc.Inventors: Randall W. Davis, Edward Shian Liu, Eric Takeshi Harada, Anne Aguirre, Andrew Trans, James Pollard, Cynthia Cech
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Patent number: 11725236Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.Type: GrantFiled: April 27, 2021Date of Patent: August 15, 2023Inventors: Randall W. Davis, Edward Shian Liu, Eric Takeshi Harada, Anne Aguirre, Andrew Trans, James Pollard, Cynthia Cech
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Publication number: 20230105456Abstract: Systems and methods for inserting a single pore into a membrane under faradaic conditions are described herein. A stepped or ramped voltage waveform can be applied across the membranes of the cells of an array, where the voltage waveform starts at first voltage and increases in magnitude over a period of time to a second voltage. The voltage waveform has a polarity that maintains a first species of a redox couple in its current oxidation state. The first voltage is selected to be low enough to reduce the risk of damaging the membrane, while the rate of voltage increase is selected to provide sufficient time for the pores to insert into the membranes. Once a pore is inserted into the membrane, the voltage across the membrane rapidly drops, thereby reducing the risk of damaging the membrane even if the applied voltage between the electrodes is further increased.Type: ApplicationFiled: December 9, 2022Publication date: April 6, 2023Inventors: Geoffrey BARRALL, Eric Takeshi HARADA, Jason David KOMADINA, J. William MANEY, JR., Charlotte YANG
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Publication number: 20210324462Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.Type: ApplicationFiled: April 27, 2021Publication date: October 21, 2021Applicant: Roche Sequencing Solutions, Inc.Inventors: Randall W. DAVIS, Edward Shian LIU, Eric Takeshi HARADA, Anne AGUIRRE, Andrew TRANS, James POLLARD, Cynthia CECH
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Patent number: 11021745Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.Type: GrantFiled: February 11, 2019Date of Patent: June 1, 2021Assignee: Roche Sequencing Solutions, Inc.Inventors: Randall W. Davis, Edward Shian Liu, Eric Takeshi Harada, Anne Aguirre, Andrew Trans, James Pollard, Cynthia Cech
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Publication number: 20190185927Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.Type: ApplicationFiled: February 11, 2019Publication date: June 20, 2019Applicant: Genia Technologies, Inc.Inventors: Randall W. DAVIS, Edward Shian LIU, Eric Takeshi HARADA, Anne AGUIRRE, Andrew TRANS, James POLLARD, Cynthia CECH
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Patent number: 10273536Abstract: A biochip for molecular detection and sensing is disclosed. The biochip includes a substrate. The biochip includes a plurality of discrete sites formed on the substrate having a density of greater than five hundred wells per square millimeter. Each discrete site includes sidewalls disposed on the substrate to form a well. Each discrete site includes an electrode disposed at the bottom of the well. In some embodiments, the wells are formed such that cross-talk between the wells is reduced. In some embodiments, the electrodes disposed at the bottom of the wells are organized into groups of electrodes, wherein each group of electrodes shares a common counter electrode. In some embodiments, the electrode disposed at the bottom of the well has a dedicated counter electrode. In some embodiments, surfaces of the sidewalls are silanized such that the surfaces facilitate the forming of a membrane in or adjacent to the well.Type: GrantFiled: March 10, 2016Date of Patent: April 30, 2019Assignees: GENIA TECHNOLOGIES, INC., ROCHE MOLECULAR SYSTEMS, INC.Inventors: Randall W. Davis, Edward Shian Liu, Eric Takeshi Harada, Anne Aguirre, Andrew Trans, James Pollard, Cynthia Cech, Hui Tian, Robert Yuan, John Foster, Roger Chen
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Patent number: 10246743Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.Type: GrantFiled: December 20, 2016Date of Patent: April 2, 2019Assignee: GENIA TECHNOLOGIES, INC.Inventors: Randall W. Davis, Edward Shian Liu, Eric Takeshi Harada, Anne Aguirre, Andrew Trans, James Pollard, Cynthia Cech
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Publication number: 20170198343Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.Type: ApplicationFiled: December 20, 2016Publication date: July 13, 2017Inventors: Randall W. DAVIS, Edward Shian LIU, Eric Takeshi HARADA, Anne AGUIRRE, Andrew TRANS, James POLLARD, Cynthia CECH
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Patent number: 9567630Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.Type: GrantFiled: October 22, 2014Date of Patent: February 14, 2017Assignee: Genia Technologies, Inc.Inventors: Randall W. Davis, Edward Shian Liu, Eric Takeshi Harada, Anne Aguirre, Andrew Trans, James Pollard, Cynthia Cech
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Publication number: 20160327507Abstract: A biochip for molecular detection and sensing is disclosed. The biochip includes a substrate. The biochip includes a plurality of discrete sites formed on the substrate having a density of greater than five hundred wells per square millimeter. Each discrete site includes sidewalls disposed on the substrate to form a well. Each discrete site includes an electrode disposed at the bottom of the well. In some embodiments, the wells are formed such that cross-talk between the wells is reduced. In some embodiments, the electrodes disposed at the bottom of the wells are organized into groups of electrodes, wherein each group of electrodes shares a common counter electrode. In some embodiments, the electrode disposed at the bottom of the well has a dedicated counter electrode. In some embodiments, surfaces of the sidewalls are silanized such that the surfaces facilitate the forming of a membrane in or adjacent to the well.Type: ApplicationFiled: March 10, 2016Publication date: November 10, 2016Inventors: Randall W. DAVIS, Edward Shian LIU, Eric Takeshi HARADA, Anne AGUIRRE, Andrew TRANS, James POLLARD, Cynthia CECH, Hui TIAN, Robert YUAN, John FOSTER, Roger CHEN
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Patent number: 9322062Abstract: A biochip for molecular detection and sensing is disclosed. The biochip includes a substrate. The biochip includes a plurality of discrete sites formed on the substrate having a density of greater than five hundred wells per square millimeter. Each discrete site includes sidewalls disposed on the substrate to form a well. Each discrete site includes an electrode disposed at the bottom of the well. In some embodiments, the wells are formed such that cross-talk between the wells is reduced. In some embodiments, the electrodes disposed at the bottom of the wells are organized into groups of electrodes, wherein each group of electrodes shares a common counter electrode. In some embodiments, the electrode disposed at the bottom of the well has a dedicated counter electrode. In some embodiments, surfaces of the sidewalls are silanized such that the surfaces facilitate the forming of a membrane in or adjacent to the well.Type: GrantFiled: October 22, 2014Date of Patent: April 26, 2016Inventors: Randall W. Davis, Edward Shian Liu, Eric Takeshi Harada, Anne Aguirre, Andrew Trans, James Pollard, Cynthia Cech, Hui Tian, Robert Yuan, John Foster, Roger Chen
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Publication number: 20150152494Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.Type: ApplicationFiled: October 22, 2014Publication date: June 4, 2015Inventors: Randall W. DAVIS, Edward Shian LIU, Eric Takeshi HARADA, Anne AGUIRRE, Andrew TRANS, James POLLARD, Cynthia CECH
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Publication number: 20150153302Abstract: A biochip for molecular detection and sensing is disclosed. The biochip includes a substrate. The biochip includes a plurality of discrete sites formed on the substrate having a density of greater than five hundred wells per square millimeter. Each discrete site includes sidewalls disposed on the substrate to form a well. Each discrete site includes an electrode disposed at the bottom of the well. In some embodiments, the wells are formed such that cross-talk between the wells is reduced. In some embodiments, the electrodes disposed at the bottom of the wells are organized into groups of electrodes, wherein each group of electrodes shares a common counter electrode. In some embodiments, the electrode disposed at the bottom of the well has a dedicated counter electrode. In some embodiments, surfaces of the sidewalls are silanized such that the surfaces facilitate the forming of a membrane in or adjacent to the well.Type: ApplicationFiled: October 22, 2014Publication date: June 4, 2015Inventors: Randall W. DAVIS, Edward Shian LIU, Eric Takeshi HARADA, Anne AGUIRRE, Andrew TRANS, James POLLARD, Cynthia CECH