Patents by Inventor Eric V. Shusta

Eric V. Shusta has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • ANTIBODIES TARGETING GLIOBLASTOMA STEM-LIKE CELLS AND METHODS OF USE THEREOF
    Publication number: 20240018263
    Abstract: The present invention provides antibodies that target glioblastoma stem-like cells and methods and kits for their use.
    Type: Application
    Filed: September 7, 2023
    Publication date: January 18, 2024
    Inventors: Michael Zorniak, Paul A. Clark, Yongku Peter Cho, Benjamin J. Umlauf, Eric V. Shusta, John Shu-Shin Kuo
  • Isogenic blood-brain barrier model
    Patent number: 11840708
    Abstract: A method of creating an isogenic multicellular blood-brain barrier model from iPSCs is disclosed.
    Type: Grant
    Filed: April 4, 2017
    Date of Patent: December 12, 2023
    Assignees: WISCONSIN ALUMNI RESEARCH FOUNDATION, CEDARS-SINAI MEDICAL CENTER
    Inventors: Eric V. Shusta, Scott G. Canfield, Clive N. Svendsen, Sean P. Palecek, Gad D. Vatine
  • METHOD OF CREATING HUMAN PLURIPOTENT STEM CELL DERIVED BRAIN PERICYTE-LIKE CELLS
    Publication number: 20230383246
    Abstract: A population of brain pericyte-like cells, wherein the cells express pericyte markers but do not express ACTA2 and wherein the cells are generated from hPSCs, is disclosed herein.
    Type: Application
    Filed: April 18, 2023
    Publication date: November 30, 2023
    Inventors: Eric V. Shusta, Sean P. Palecek, Matthew Stebbins
  • HUMAN BLOOD-BRAIN BARRIER MODEL FOR IMMUNOLOGICAL STUDIES
    Publication number: 20230375530
    Abstract: The present invention provides methods for differentiating brain microvascular endothelial cells having barrier properties and a mature immune phenotype for use in making an in vitro blood-brain barrier (BBB) model. Further, a BBB model having barrier properties and a mature immune phenotype is provided.
    Type: Application
    Filed: September 28, 2021
    Publication date: November 23, 2023
    Inventors: Eric V. SHUSTA, Hideaki NISHIHARA, Benjamin GASTFRIEND, Sean P. PALECEK, Britta ENGELHARDT
  • HUMAN BLOOD-BRAIN BARRIER TARGETING ANTIBODIES
    Publication number: 20230331835
    Abstract: The present invention provides antibodies or antigen-binding fragments thereof including single chain variable fragment (scFv) antibodies that specifically bind to and translocate the blood-brain barrier and methods of use.
    Type: Application
    Filed: January 28, 2021
    Publication date: October 19, 2023
    Inventors: Eric V. SHUSTA, Charles C. STUTZ, Loukas GOULATIS, Julia GEORGIEVA
  • Antibodies targeting glioblastoma stem-like cells and methods of use thereof
    Patent number: 11780932
    Abstract: The present invention provides antibodies that target glioblastoma stem-like cells and methods and kits for their use.
    Type: Grant
    Filed: October 9, 2018
    Date of Patent: October 10, 2023
    Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: Michael Zorniak, Paul A. Clark, Yongku Peter Cho, Benjamin J. Umlauf, Eric V. Shusta, John Shu-Shin Kuo
  • Method of creating human pluripotent stem cell derived brain pericyte-like cells
    Patent number: 11643636
    Abstract: A population of brain pericyte-like cells, wherein the cells express pericyte markers but do not express ACTA2 and wherein the cells are generated from hPSCs, is disclosed herein.
    Type: Grant
    Filed: July 10, 2019
    Date of Patent: May 9, 2023
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Eric V. Shusta, Sean P. Palecek, Matthew Stebbins
  • VARIABLE LYMPHOCYTE RECEPTORS THAT TARGET THE BLOOD BRAIN BARRIER AND METHODS OF USE
    Publication number: 20220204583
    Abstract: The present disclosure provides isolated polypeptides comprising variable lymphocyte receptors that specifically bind the blood brain barrier, compositions, and methods of use.
    Type: Application
    Filed: February 11, 2020
    Publication date: June 30, 2022
    Inventors: Eric V. Shusta, Jason M. Lajoie, Brantley R. Herrin
  • VARIABLE LYMPHOCYTE RECEPTORS THAT TARGET THE BRAIN EXTRACELLULAR MATRIX AND METHODS OF USE
    Publication number: 20220041659
    Abstract: The present disclosure provides isolated polypeptides comprising variable lymphocyte receptors that specifically bind the brain extracellular matrix, compositions, and methods of use. Methods of using the variable lymphocyte receptors for the detection and treatment of disease or injury, specifically, for example, cancers including glioblastoma are provided.
    Type: Application
    Filed: December 19, 2019
    Publication date: February 10, 2022
    Inventors: Eric V. SHUSTA, Benjamin UMLAUF, Brantley HERRIN, Paul CLARK, John KUO
  • BLOOD BRAIN BARRIER SELECTIVE ANTIBODIES AND METHODS OF USE
    Publication number: 20210277108
    Abstract: The present invention provides antibodies or antigen binding fragments thereof including single chain variable fragment (scFv) antibodies that specifically bind to the blood brain barrier in vivo and methods of use.
    Type: Application
    Filed: July 24, 2019
    Publication date: September 9, 2021
    Inventors: Eric V. SHUSTA, Charles C. STUTZ
  • ANTIBODIES TARGETING GLIOBLASTOMA STEM-LIKE CELLS AND METHODS OF USE THEREOF
    Publication number: 20200354472
    Abstract: The present invention provides antibodies that target glioblastoma stem-like cells and methods and kits for their use.
    Type: Application
    Filed: October 9, 2018
    Publication date: November 12, 2020
    Applicant: Wisconsin Alumni Research Foundation
    Inventors: Michael Zorniak, Paul A. Clark, Yongku Peter Cho, Benjamin J. Umlauf, Eric V. Shusta, John Shu-Shin Kuo
  • Retinoic acid enhanced human stem cell derived blood brain barrier model
    Patent number: 10590393
    Abstract: In one embodiment, the present invention is a method of creating a fully-human blood-brain barrier (BBB) model, comprising the steps of (a) obtaining a mixture of neural cells and brain microvascular endothelial cells (BMECs), wherein the neural cells and BMECs that comprise the mixture were produced from the differentiation of human pluripotent stem cells (hPSCs); (b) purifying BMECs from the mixture of neural cells and BMECs of step (a); and (c) co-culturing the purified BMECs with a cell type selected from the group consisting of pericytes, astrocytes and differentiated neural progenitor cells (NPCs), wherein a blood brain barrier model is created.
    Type: Grant
    Filed: March 11, 2013
    Date of Patent: March 17, 2020
    Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: Eric V. Shusta, Sean P. Palecek, Ethan S. Lippmann, Samira M. Azarin
  • METHOD OF CREATING HUMAN PLURIPOTENT STEM CELL DERIVED BRAIN PERICYTE-LIKE CELLS
    Publication number: 20200017827
    Abstract: A population of brain pericyte-like cells, wherein the cells express pericyte markers but do not express ACTA2 and wherein the cells are generated from hPSCs, is disclosed herein.
    Type: Application
    Filed: July 10, 2019
    Publication date: January 16, 2020
    Inventors: Eric V. Shusta, Sean P. Palecek, Matthew Stebbins
  • ISOGENIC BLOOD-BRAIN BARRIER MODEL
    Publication number: 20190093084
    Abstract: A method of creating an isogenic multicellular blood-brain barrier model from iPSCs is disclosed.
    Type: Application
    Filed: April 4, 2017
    Publication date: March 28, 2019
    Inventors: Eric V. Shusta, Scott G. Canfield, Clive N. Svendsen
  • pH-dependent antibodies targeting the transferrin receptor and methods of use thereof to deliver a therapeutic agent
    Patent number: 10233252
    Abstract: A pH-dependent antibody that binds an antigen with high affinity at a first pH and rapidly dissociates at a second pH, wherein the antigen is a transferrin receptor (TfR), wherein the association at the second pH/the first pH is less than 20%, and wherein the pH-dependent antibody comprises at least two consecutive histidine residues at a single complementarity determining region (CDR) is disclosed.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: March 19, 2019
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Eric V. Shusta, Benjamin J. Tillotson
  • Methods for differentiation of human pluripotent stem cells to brain microvascular endothelial cells
    Patent number: 10214724
    Abstract: Methods for generating functional brain microvascular endothelial cells (BMECs) under chemically defined, serum-free conditions are provided. In particular, efficient and cost-effective methods for generating functional BMECs under chemically defined culture conditions are provided. BMECs obtained according to the methods provided herein are suitable for in vitro blood brain barrier (BBB) formation.
    Type: Grant
    Filed: April 4, 2017
    Date of Patent: February 26, 2019
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Tongcheng Qian, Eric V. Shusta, Sean P. Palecek
  • METHOD OF DIFFERENTIATING HUMAN PLURIPOTENT STEM CELLS TO PODOCYTES
    Publication number: 20190055520
    Abstract: The present invention provides methods and kits for differentiating podocytes from pluripotent stem cells and from other cell types.
    Type: Application
    Filed: August 20, 2018
    Publication date: February 21, 2019
    Inventors: Tongcheng Qian, Sean P. Palecek, Eric V. Shusta
  • Human blood-brain barrier endothelial cells derived from pluripotent stem cells and blood-brain barrier model thereof
    Patent number: 9902940
    Abstract: A model blood brain barrier obtained from hPSCs is disclosed.
    Type: Grant
    Filed: June 8, 2011
    Date of Patent: February 27, 2018
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Eric V. Shusta, Samira Azarin, Sean Palecek, Ethan Lippmann
  • METHODS FOR DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELLS TO BRAIN MICROVASCULAR ENDOTHELIAL CELLS
    Publication number: 20170283772
    Abstract: Methods for generating functional brain microvascular endothelial cells (BMECs) under chemically defined, serum-free conditions are provided. In particular, efficient and cost-effective methods for generating functional BMECs under chemically defined culture conditions are provided. BMECs obtained according to the methods provided herein are suitable for in vitro blood brain barrier (BBB) formation.
    Type: Application
    Filed: April 4, 2017
    Publication date: October 5, 2017
    Inventors: Tongcheng Qian, Eric V. Shusta, Sean P. Palecek
  • Engineered intein for improved production of protein-intein fusions
    Patent number: 9771412
    Abstract: The invention discloses engineered non-self-cleaving inteins derived from Mxe GyrA inteins and methods of using such inteins to chemically modify proteins.
    Type: Grant
    Filed: October 29, 2015
    Date of Patent: September 26, 2017
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Eric V. Shusta, Carrie J. Marshall