Abstract: The invention relates to a method for producing a fructose composition comprising the following successive stages: provision of an initial composition comprising glucose; concentration of the initial composition by evaporation of water to obtain a concentrated initial composition; isomerization of glucose to fructose from the concentrated initial composition, making it possible to obtain an intermediate composition; purification of the intermediate composition in a multicolumn chromatography system, making it possible to obtain a glucose-rich raffinate and a fructose-rich extract; concentration of the extract by evaporation of water; wherein the intermediate composition is not subjected to a concentration step by evaporation of water between the isomerization step and the purification step.

Abstract: The invention relates to a method for purifying a mixture to be separated, in a multicolumn chromatography system, the method comprising successively and cyclically: a step of collecting a raffinate, a step of injecting the mixture to be separated, a step of collecting an extract and an eluent injection step, at an operating temperature; wherein the mixture to be separated has a viscosity at 20° C. greater than or equal to 3 mPa·s; and wherein the dry matter mass concentration of the mixture to be separated is equal, within 5%, to a threshold concentration, said threshold concentration is such that: the viscosity of the mixture to be treated at a dry matter mass concentration equal to the threshold concentration and at the operating temperature, is equal to twice the viscosity of the mixture to be treated, at a dry matter mass concentration equal to 85% of the threshold concentration and at the operating temperature.

Abstract: The invention relates to a method for separating a mixture in a system comprising a plurality of chromatography columns, the method successively comprising, in a cyclical manner, in a given part of the system: a step of collecting a raffinate; a step of injecting the mixture to be separated; a step of collecting an extract; and a step of injecting a mobile phase; the method further comprising: determining, in a node of the system, the history of a variable representing the concentration of at least one species contained in the mixture to be separated; detecting, within said history, a characteristic point between the beginning of a step of collecting the extract and the end of the following step of collecting the raffinate; comparing the position of the characteristic point in relation to a target position; and adjusting the carrier volume of the characteristic point, modifying the position of the characteristic point in order to bring the position of the characteristic point closer to the target position; th

Abstract: The invention relates to an apparatus for purification of biomolecules, comprising an injection device comprising an injection channel provided with at least one inlet for a precipitation agent composition and a plurality of outlets on the external surface of the injection channel and a mixing device comprising a mixing channel with at least one main inlet for a composition to be purified and at least one main outlet, and a plurality of secondary inlets, wherein the injection device and the mixing device are coupled such that each of the plurality of outlets of the injection device is connected to a respective secondary inlet of the mixing channel. The invention further relates to a set comprising the apparatus, a composition comprising at least one biomolecule and at least one impurity, and a composition comprising at least one precipitation agent, and to a method for purification of biomolecules using the apparatus.

Abstract: The invention relates to a method for producing a fructose composition comprising the following successive stages: provision of an initial composition comprising glucose; concentration of the initial composition by evaporation of water to obtain a concentrated initial composition; isomerization of glucose to fructose from the concentrated initial composition, making it possible to obtain an intermediate composition; purification of the intermediate composition in a multicolumn chromatography system, making it possible to obtain a glucose-rich raffinate and a fructose-rich extract; concentration of the extract by evaporation of water; wherein the intermediate composition is not subjected to a concentration step by evaporation of water between the isomerization step and the purification step.

Abstract: The invention relates to a method for purifying a mixture to be separated, in a multicolumn chromatography system, the method comprising successively and cyclically: a step of collecting a raffinate, a step of injecting the mixture to be separated, a step of collecting an extract and an eluent injection step, at an operating temperature; wherein the mixture to be separated has a viscosity at 20° C. greater than or equal to 3 mPa·s; and wherein the dry matter mass concentration of the mixture to be separated is equal, within 5%, to a threshold concentration, said threshold concentration is such that: the viscosity of the mixture to be treated at a dry matter mass concentration equal to the threshold concentration and at the operating temperature, is equal to twice the viscosity of the mixture to be treated, at a dry matter mass concentration equal to 85% of the threshold concentration and at the operating temperature.

Abstract: The invention relates to a method for separating a mixture in a system comprising a plurality of chromatography columns, the method successively comprising, in a cyclical manner, in a given part of the system: a step of collecting a raffinate; a step of injecting the mixture to be separated; a step of collecting an extract; and a step of injecting a mobile phase; the method further comprising: determining, in a node of the system, the history of a variable representing the concentration of at least one species contained in the mixture to be separated; detecting, within said history, a characteristic point between the beginning of a step of collecting the extract and the end of the following step of collecting the raffinate; comparing the position of the characteristic point in relation to a target position; and adjusting the carrier volume of the characteristic point, modifying the position of the characteristic point in order to bring the position of the characteristic point closer to the target position; th

Abstract: The invention relates to a method for purifying a solution, the method comprising the following successive steps: —bringing a solution to be purified into contact with an ion exchange resin by suspending the ion exchange resin in the solution to be purified, the ion exchange resin having the form of particles having a size Dv50 smaller than or equal to 200 ?m; —separating the solution into a purified solution and a loaded resin; —regenerating the loaded resin by passing at least one regenerating solution through a compact bed of loaded resin. The invention also relates to an assembly for implementing the method.

Abstract: The invention relates to a fluid distributor (9, 10) for a column (1) comprising at least one cell (2) containing a bed of particles (3), the fluid distributor (9, 10) comprising: —a floor (11, 21) comprising at least one injection point (15, 25) providing a fluidic connection on either side thereof; —a layer of a resistive medium (12, 22) fixed on one face of the floor (11, 21), the resistive medium being permeable to the fluid; —a spacing device (13, 23) inserted between the floor (11, 21) and the layer of resistive medium (12, 22) and maintaining a space for the circulation of the fluid between the floor (11, 21) and the layer of resistive medium (12, 22). The invention also relates to a column, in particular a chromatography column, provided with this fluid distributor.

Abstract: The invention relates to a method for purifying a solution, the method comprising the following successive steps: —bringing a solution to be purified into contact with an ion exchange resin by suspending the ion exchange resin in the solution to be purified, the ion exchange resin having the form of particles having a size Dv50 smaller than or equal to 200 ?m; —separating the solution into a purified solution and a loaded resin; —regenerating the loaded resin by passing at least one regenerating solution through a compact bed of loaded resin. The invention also relates to an assembly for implementing the method.

Abstract: The invention relates to a fluid distributor (9,10)for a column (1)comprising at least one cell (2) containing a bed of particles (3), the fluid distributor (9, 10) comprising: - a floor (11, 21) comprising at least one injection point (15, 25) providing a fluidic connection on either side thereof; - a layer of resistive medium (12, 22) fixed on one face of the floor(11, 21), the resistive medium being permeable to the fluid; - a spacing device (13, 23) inserted between the floor (11, 21) and the layer of reistive medium (12, 22) and maintaining a space for the circulation of the fluid between the floor (11, 21) and the layer of resistive medium (12, 22). The invention also relates to a column, in particular a chromatography column, provided with this fluid distributor.

Abstract: A read circuit for an electrical signal produced by a POSFET device is disclosed which comprises a transconductance amplifier (4) connected at its inverting input to the output of the POSFET device (1), at least one neuron (8a, 8b) connected to the output of said transconductance amplifier, said amplifier being adapted to receive a signal from the POSFET device representative of a force or pressure exerted on it and to produce at its output at least one current signal (I0?, I0+) representative of such force or pressure, the at least one neuron being adapted to receive said current signal and to produce an output signal (12a, 12b) being a pulse train with frequency proportional to said current signal.

Abstract: The invention concerns a method for separating fractions of a mixture to be separated, in a device (1) comprising: several chromatography columns (21, 22, 23,) mounted in series; an open separation loop comprising in input a point for injecting (6) eluent in one of the columns and in output a point for drawing (8) a fraction of the mixture. The method includes the following steps: batch injection of the mixture to be separated in the open separation loop (4); collecting at least two fractions; offsetting at least one column of the eluent injecting and fraction drawing points (6, 8) of the separation loop (4). The invention also concerns a device for separating fractions of a mixture to be separated.

Abstract: The invention relates to a method for separating fractions of a mixture to be separated including the steps of providing a device comprising at least two chromatography columns mounted in series, discontinuously injecting a mixture to be separated, in a single point of the device, discontinuously drawing off the fraction enriched in the product of interest, in a single point of the device, and injecting eluents in each column and displacing the points for injection eluents, the eluents having a different eluting power. The method allows efficient separation which is simple to perform.

Abstract: A process is disclosed for separating biomolecules from an aqueous solution containing the biomolectules and impurities, having different affinities and/or interactions with a solid support. The solution is passed over a fixed bed of chromatographic resin containing at least three zones, with flow of liquid being arranged between adjacent zones and between a last and first zone. Each of several sequences includes at least an adsorption stage, a rinsing stage, or a desorption stage, with each subsequent sequence being carried out by a downstream displacement of fronts in the zones by approximately the same increment before the periodical displacement of the introduction and withdrawal points.

Abstract: A process is disclosed for separating biomolecules from an aqueous solution containing the biomolecules and impurities, having different affinities and/or interactions with a solid support. The solution is passed over a fixed bed of chromatographic resin containing at least three zones, with flow of liquid being arranged between adjacent zones and between a last and first zone. Each of several sequences includes at least an adsorption stage, a rinsing stage, or a desorption stage, with each subsequent sequence being carried out by a downstream displacement of fronts in the zones by approximately the same increment before the periodical displacement of the introduction and withdrawal points.

Abstract: The invention concerns a method for separating fractions of a mixture using a chromatography device. The method includes the following steps: controlling in one node of the device, the history of a specific variable of the fractions of the mixture to be separated; detecting one characteristic point of the history, the characteristic point being between two successive steps of fraction collecting; comparing the position of the characteristic point relative to a target position; adjusting the amount of mobile phase modifying the position of the characteristic point to cause the position of the characteristic point to coincide with the target position. The invention also concerns a device for implementing the method. The method enables the operation of the device to be automatically set.

Abstract: A process is disclosed for separating biomolecules from an aqueous solution containing the biomolectules and impurities, having different affinities and/or interactions with a solid support. The solution is passed over a fixed bed of chromatographic resin containing at least three zones, with flow of liquid being arranged between adjacent zones and between a last and first zone. Each of several sequences includes at least an adsorption stage, a rinsing stage, or a desorption stage, with each subsequent sequence being carried out by a downstream displacement of fronts in the zones by approximately the same increment before the periodical displacement of the introduction and withdrawal points.

Abstract: The invention concerns a method for separating fractions of a mixture to be separated, in a device (1) comprising: several chromatography columns (21, 22, 23,) mounted in series; an open separation loop comprising in input a point for injecting (6) eluent in one of the columns and in output a point for drawing (8) a fraction of the mixture. The method includes the following steps: batch injection of the mixture to be separated in the open separation loop (4); collecting at least two fractions; offsetting at least one column of the eluent injecting and fraction drawing points (6, 8) of the separation loop (4). The invention also concerns a device for separating fractions of a mixture to be separated.

Abstract: The invention relates to a method for separating fractions of a mixture to be separated comprising the steps of providing a device comprising at least two chromatography columns mounted in series, discontinuously injecting a mixture to be separated, in a single point of the device, discontinuously drawing off the fraction enriched in the product of interest, in a single point of the device, injecting eluents in each column and displacing the points for injecting eluents, the eluents having a different eluting power. The method allows efficient separation which is simple to perform.