Patents by Inventor Eric Van Der Walt
Eric Van Der Walt has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240132942Abstract: Disclosed herein are methods and compositions for normalizing polynucleotide concentration. Normalizing may be accomplished using polynucleotide binding proteins (e.g., catalytically inactive CRISPR protein or catalytically inactive Argonaute proteins). The polynucleotide binding proteins may bind to the adapter sequences of a target polynucleotide. Thus, adding the same amount of a polynucleotide binding proteins to different samples and then extracting the polynucleotide protein is shown herein to extract similar amounts of target polynucleotides from the different samples.Type: ApplicationFiled: October 22, 2023Publication date: April 25, 2024Applicant: Watchmaker Genomics, Inc.Inventors: Martin Ranik, Eric van der Walt, Clara Ross, Craig Marshall, Lindsay Peterkin, Brian Kudlow
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Patent number: 11932847Abstract: Disclosed is a method of fragmenting DNA comprising contacting a sample of target DNA with (a) a composition comprising an active transpososome, and (b) a composition comprising an inactive transpososome, under conditions suitable for transpososome activity, wherein a ratio of an amount of the inactive transpososome in the composition of (b) to an amount of the active transpososome in the composition of (a) determines the mean fragment size and a level of insertion bias.Type: GrantFiled: April 19, 2017Date of Patent: March 19, 2024Assignee: KAPA BIOSYSTEMS, INC.Inventors: Martin Ranik, Eric Van der Walt, William Bourn, Jennifer Hsieh, Abre De Beer, Gerrida Uys, Paul McEwan
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Patent number: 11746339Abstract: The present invention provides improved DNA polymerases that may be better suited for applications in recombinant DNA technologies, in particular technologies involving plant-derived samples. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.Type: GrantFiled: March 11, 2021Date of Patent: September 5, 2023Assignee: Kapa Biosystems, Inc.Inventors: Wolfgang Schafer, Paul J. McEwan, Eric van der Walt, John Foskett, William Bourn
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Publication number: 20220380823Abstract: The disclosure provides a composition comprising a double-stranded deoxyribonucleic acid (dsDNA) sequence comprising from 5? to 3?, a sequence comprising a first adaptor sequence, a template sequence, and a second adaptor sequence, wherein the second adaptor sequence comprises a hybridization site for a template switching oligonucleotide (TSO). The disclosure provides methods for making the compositions of the disclosure using a template switching mechanism to add non-templated basepairs to the ends of a DNA molecule, hybridize a TSO to the non-templated basepairs, and then extend the sequence complementary to the TSO to add an adaptor.Type: ApplicationFiled: July 19, 2022Publication date: December 1, 2022Inventors: Jennifer Hsieh, Paul McEwan, Martin Ranik, Marliz Iain McAllister Strydom, Eric van der Walt, Ross Wadsworth
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Patent number: 11427846Abstract: The disclosure provides a composition comprising a double-stranded deoxyribonucleic acid (dsDNA) sequence comprising from 5? to 3?, a sequence comprising a first adaptor sequence, a template sequence, and a second adaptor sequence, wherein the second adaptor sequence comprises a hybridization site for a template switching oligonucleotide (TSO). The disclosure provides methods for making the compositions of the disclosure using a template switching mechanism to add non-templated basepairs to the ends of a DNA molecule, hybridize a TSO to the non-templated basepairs, and then extend the sequence complementary to the TSO to add an adaptor.Type: GrantFiled: June 4, 2020Date of Patent: August 30, 2022Assignee: Kapa Biosystems, Inc.Inventors: Jennifer Hsieh, Paul McEwan, Martin Ranik, Marliz Strydom, Eric van der Walt, Ross Wadsworth
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Publication number: 20210317422Abstract: The present invention provides improved DNA polymerases that may be better suited for applications in recombinant DNA technologies, in particular technologies involving plant-derived samples. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.Type: ApplicationFiled: March 11, 2021Publication date: October 14, 2021Inventors: Wolfgang Schafer, Paul J. McEwan, Eric van der Walt, John Foskett, William Bourn
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Patent number: 11001834Abstract: The present invention provides synthetic nucleic acid molecule tags that can be added into samples for identification and tracking. Among other things, the present invention provides synthetic, high-molecular weight concatemers, which can be combined with samples to yield hundreds of millions of unique identifiers. Examples applications for which the synthetic nucleic acid molecule tags can be used, include industrial, research and clinical applications.Type: GrantFiled: September 28, 2016Date of Patent: May 11, 2021Assignee: KAPA BIOSYSTEMS, INC.Inventors: Paul McEwan, Martin Ranik, Eric van der Walt
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Patent number: 10975361Abstract: The present invention provides improved DNA polymerases that may be better suited for applications in recombinant DNA technologies, in particular technologies involving plant-derived samples. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.Type: GrantFiled: April 15, 2016Date of Patent: April 13, 2021Assignee: Kapa Biosystems, Inc.Inventors: Wolfgang Schafer, Paul McEwan, Eric Van Der Walt, John Foskett, William Bourn
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Publication number: 20200299764Abstract: The present invention provides a method for targeted enrichment of nucleic acids including contacting a nucleic acid including at least one region of interest with a plurality of transposase complexes. Each of the transposase complexes includes at least a transposase and a first polynucleotide having a transposon end sequence and a first label sequence. The method further includes incubating the nucleic acid and the transposase complexes under conditions whereby the nucleic acid is fragmented into a plurality of nucleic acid fragments including first polynucleotide attached to each 5? end of the nucleic acid fragments. The method further includes selectively amplifying the nucleic acid fragments, thereby enriching for a portion of the nucleic acid fragments including the at least one region of interest relative to a remaining portion of the nucleic acid fragments, and sequencing the enriched nucleic acid fragments.Type: ApplicationFiled: April 29, 2020Publication date: September 24, 2020Inventors: Bronwen Miller, Jo-Anne Elizabeth Penkler, Martin Ranik, Eric van der Walt
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Publication number: 20200291440Abstract: The disclosure provides a composition comprising a double-stranded deoxyribonucleic acid (dsDNA) sequence comprising from 5? to 3?, a sequence comprising a first adaptor sequence, a template sequence, and a second adaptor sequence, wherein the second adaptor sequence comprises a hybridization site for a template switching oligonucleotide (TSO). The disclosure provides methods for making the compositions of the disclosure using a template switching mechanism to add non-templated basepairs to the ends of a DNA molecule, hybridize a TSO to the non-templated basepairs, and then extend the sequence complementary to the TSO to add an adaptor.Type: ApplicationFiled: June 4, 2020Publication date: September 17, 2020Inventors: Jennifer Hsieh, Paul McEwan, Martin Ranik, Marliz Strydom, Eric van der Walt, Ross Wadsworth
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Publication number: 20180195059Abstract: Disclosed is a method of fragmenting DNA comprising contacting a sample of target DNA with (a) a composition comprising an active transpososome, and (b) a composition comprising an inactive transpososome, under conditions suitable for transpososome activity, wherein a ratio of an amount of the inactive transpososome in the composition of (b) to an amount of the active transpososome in the composition of (a) determines the mean fragment size and a level of insertion bias.Type: ApplicationFiled: April 19, 2017Publication date: July 12, 2018Inventors: Martin RANIK, Eric VAN DER WALT, William BOURN, Jennifer HSIEH
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Publication number: 20180016632Abstract: The present invention provides a method for targeted enrichment of nucleic acids including contacting a nucleic acid including at least one region of interest with a plurality of transposase complexes. Each of the transposase complexes includes at least a transposase and a first polynucleotide having a transposon end sequence and a first label sequence. The method further includes incubating the nucleic acid and the transposase complexes under conditions whereby the nucleic acid is fragmented into a plurality of nucleic acid fragments including first polynucleotide attached to each 5? end of the nucleic acid fragments. The method further includes selectively amplifying the nucleic acid fragments, thereby enriching for a portion of the nucleic acid fragments including the at least one region of interest relative to a remaining portion of the nucleic acid fragments, and sequencing the enriched nucleic acid fragments.Type: ApplicationFiled: July 12, 2017Publication date: January 18, 2018Inventors: Jo-Anne PENKLER, Bronwen MILLER, Martin RANIK, Eric van der WALT
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Publication number: 20170088832Abstract: The present invention provides synthetic nucleic acid molecule tags that can be added into samples for identification and tracking. Among other things, the present invention provides synthetic, high-molecular weight concatemers, which can be combined with samples to yield hundreds of millions of unique identifiers. Examples applications for which the synthetic nucleic acid molecule tags can be used, include industrial, research and clinical applications.Type: ApplicationFiled: September 28, 2016Publication date: March 30, 2017Inventors: Paul McEwan, Martin Ranik, Eric Van Der Walt
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Publication number: 20170067037Abstract: The present invention provides improved DNA polymerases that may be better suited for applications in recombinant DNA technologies, in particular technologies involving plant-derived samples. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.Type: ApplicationFiled: April 15, 2016Publication date: March 9, 2017Inventors: Wolfgang Schafer, Paul McEwan, Eric Van Der Walt, John Foskett, William Bourn
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Patent number: 9315787Abstract: The present invention provides improved DNA polymerases that may be better suited for applications in recombinant DNA technologies, in particular technologies involving plant-derived samples. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.Type: GrantFiled: January 13, 2012Date of Patent: April 19, 2016Assignee: KAPA BIOSYSTEMS, INC.Inventors: Wolfgang Schafer, Paul McEwan, Eric Van Der Walt, John Foskett, William Bourn
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Publication number: 20140030765Abstract: The present invention provides improved DNA polymerases that may be better suited for applications in recombinant DNA technologies, in particular technologies involving plant-derived samples. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.Type: ApplicationFiled: January 13, 2012Publication date: January 30, 2014Inventors: Wolfgang Schafer, Paul McEwan, Eric Van Der Walt, John Foskett, William Bourn
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Publication number: 20110294167Abstract: The present invention provides improved systems and methods for amplifying nucleic acids. Among other things the present invention provides a system for amplifying nucleic acids through use of a primase and a polymerase with strand-displacement ability without, for example, exogenously-added primers. The present invention is particularly useful for whole genome amplification.Type: ApplicationFiled: December 2, 2009Publication date: December 1, 2011Applicant: KAPABIOSYSTEMSInventors: Paul McEwan, Bjarne Faurholm, Eric Van Der Walt