Patents by Inventor Eric W. Overstrom

Eric W. Overstrom has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • OOCYTE SPINDLE-ASSOCIATED FACTORS IMPROVE SOMATIC CELL CLONING
    Publication number: 20100205678
    Abstract: The invention pertains to the discovery that the presence of oocyte spindle associated factors in an enucleated oocyte improves oocyte quality and subsequently nuclear transfer. In particular, it was discovered that maintaining oocyte spindle factors in the oocyte after enucleation improves oocyte quality for use in nuclear transfer methodology.
    Type: Application
    Filed: January 4, 2008
    Publication date: August 12, 2010
    Inventors: Eric W. Overstrom, Elena Ibáñez, Christine A. Lima
  • Nuclear transfer embryo formation method
    Patent number: 7612250
    Abstract: A nuclear transfer embryo is formed by destabilizing microtubules of an oocyte, whereby essentially all endogenous chromatin collects at a second polar body during meiosis of an oocyte. The oocyte is fused with the nucleus of a donor somatic cell of the same species of said oocyte prior to cessation of extrusion of the second polar body from the oocyte, thereby forming the nuclear transfer embryo. In one embodiment, the nuclear transfer embryo is employed to impregnate an animal, such as a mammal. In another embodiment, the donor nucleus is transgenic.
    Type: Grant
    Filed: July 29, 2002
    Date of Patent: November 3, 2009
    Assignee: Trustees of Tufts College
    Inventors: Eric W. Overstrom, Daniela Fischer Russell
  • Mammalian telophase oocyte enucleation
    Patent number: 7592503
    Abstract: The present invention pertains to methods for cloning animals. In particular, the invention includes methods of cloning an animal by combining a genome from an activated donor cell with an activated enucleated oocyte to thereby obtain a nuclear transfer embryo, and impregnating an animal with the nuclear transfer embryo in conditions suitable for gestation of a cloned animal. The invention further relates to methods of chemically enucleating an oocyte having a meiotic spindle apparatus by exposing the oocyte with a compound that destabilizes the meiotic spindle apparatus.
    Type: Grant
    Filed: August 6, 2004
    Date of Patent: September 22, 2009
    Assignee: Trustees of Tufts College
    Inventors: Alexander Baguisi, Eric W. Overstrom
  • Methods for cloning animals
    Publication number: 20050144663
    Abstract: The present invention pertains to methods for cloning animals. In particular, the invention includes methods of cloning an animal by combining a genome from an activated donor cell with an activated enucleated oocyte to thereby obtain a nuclear transfer embryo, and impregnating an animal with the nuclear transfer embryo in conditions suitable for gestation of a cloned animal. The invention further relates to methods of chemically enucleating an oocyte having a meiotic spindle apparatus by exposing the oocyte with a compound that destabilizes the meiotic spindle apparatus.
    Type: Application
    Filed: August 6, 2004
    Publication date: June 30, 2005
    Inventors: Alexander Baguisi, Eric W. Overstrom
  • Methods for cloning mammals using telophase oocytes
    Patent number: 6781030
    Abstract: The present invention pertains to methods for cloning animals. In particular, the invention includes methods of cloning an animal by combining a genome from an activated donor cell with an activated enucleated oocyte to thereby obtain a nuclear transfer embryo, and impregnating an animal with the nuclear transfer embryo in conditions suitable for gestation of a cloned animal. The invention further relates to methods of chemically enucleating an oocyte having a meiotic spindle apparatus by exposing the oocyte with a compound that destabilizes the meiotic spindle apparatus.
    Type: Grant
    Filed: November 2, 1999
    Date of Patent: August 24, 2004
    Assignee: Trustee of Tufts College, Ballou Hall
    Inventors: Alexander Baguisi, Eric W. Overstrom
  • Nuclear transfer embryo formation method
    Publication number: 20040019924
    Abstract: A nuclear transfer embryo is formed by destabilizing microtubules of an oocyte, whereby essentially all endogenous chromatin collects at a second polar body during meiosis of an oocyte. The oocyte is fused with the nucleus of a donor somatic cell of the same species of said oocyte prior to cessation of extrusion of the second polar body from the oocyte, thereby forming the nuclear transfer embryo. In one embodiment, the nuclear transfer embryo is employed to impregnate an animal, such as a mammal. In another embodiment, the donor nucleus is transgenic.
    Type: Application
    Filed: July 29, 2002
    Publication date: January 29, 2004
    Applicant: The Trustees of Tufts College
    Inventors: Eric W. Overstrom, Daniela Fischer