Abstract: A purified acarbose which contains less than 10% by weight of sugar-like secondary components is obtained by column chromatograph of a solution of prepurified acarbose with a pH 4 to 7. The column contains as a packing material a weakly acid cation exchanger which has carboxyl groups and is based on dextran, agarose and cellulose or exchangers which are derived from the latter with the addition of polyamide.

Abstract: In the purification of acarbose by contacting an acarbose-containing solution with a cation exchanger to adsorb the acarbose, eluting the ion exchanger and collecting an eluate fraction enriched in purified acarbose, the improvement wherein the cation exchanger is a polymer obtained by polymerizing an aromatic compound possessing at least one vinyl group and at least one hydrophilic monomer in the presence of a solvent for the monomer which is a precipitant for the crosslinked polymer formed, isolating the resistant polymer, and sulphonating the polymer in the presence of a swelling agent for the polymer. The invention gives sharp separation and other processing advantages.

Abstract: In the purification of acarbose by contacting an acarbose-containing solution with a cation exchanger to adsorb the acarbose, eluting the ion exchanger and collecting an eluate fraction enriched in purified acarbose, the improvement wherein the cation exchanger is a polymer obtained by polymerizing an aromatic compound possessing at least one vinyl group and at least one hydrophilic monomer in the presence of a solvent for the monomer which is a precipitant for the crosslinked polymer formed, isolating the resistant polymer, and sulphonating the polymer in the presence of a swelling agent for the polymer. The invention gives shapr separation and other processing advantages.

Abstract: The invention relates to a process for isolating O-{4,6-dideoxy-4-[[1S-(1,4,6/5)-4,5,6-trihydroxy-3-hydroxymethyl-2-cyclohe xen-1-yl]-amino]-.alpha.-D-glucopyranosyl}-(1.fwdarw.4)-O-.alpha.-D-glucopy ranosyl-(1.fwdarw.4)-D-glucopyranose from culture broths such as are obtained by fermenting micro-organisms of the family Actinoplanaceae, especially of strains of the genus Actinoplanes involving the use of ion exchangers.

Abstract: There are disclosed water-insoluble proteinic preparations in which a protein or polypeptide is bound to a copolymer, which preparations are useful for binding enzymes in order to carry out enzyme-catalyzable reactions. These preparations comprise a peptide material bound to a cross-linked copolymer comprising the following copolymerized units:A. about 0.1 to 50 weight percent of at least one .alpha.,.beta. - monoolefinically unsaturated dicarboxylic acid anhydride having about 4 to 9 carbon atoms;B. about 99.9 to 50 weight percent of at least one di- and/or poly(meth)acrylate of a diol and/or a polyol as hereinafter defined,The copolymer having a bulk volume of 1.4 to 30 ml/g and a specific surface area of about 1 to 500 m.sup.2 /g, and containing, after saponification of the anhydride groups, about 0.02 to 10 milliequivalents of acid per gram.

Abstract: There are disclosed water-insoluble proteinic preparations in which a protein or polypeptide is bound to a copolymer, which preparations are useful for binding enzymes in order to carry out enzyme-catalyzable reactions. These preparations comprise a peptide material bound to a crosslinked copolymer comprising the following copolymerized units:A. about 0.1 to 30 wt. % of at least one .alpha.,.beta.-monoolefinically unsaturated dicarboxylic acid anhydride having about 4 to 9 carbon atoms;B. about 35 to 90 wt. % of at least one di- and/or poly(meth)acrylate of a diol and/or polyol as hereinafter defined; andC. about 5 to 60 wt. % of at least one hydrophilic monomer,The copolymer having a bulk volume of 1.4 to 30 ml/g and a specific surface area of about 1 to 500 m.sup.2 /g, and containing, after saponification of the anhydride groups, about 0.01 to 14 milliequivalents of acid per gram.