Abstract: The present invention relates to silk fibroin bioinks specifically formulated for use in 3D bioprinting and the production of ex-vivo models capable of supporting hematopoiesis and the production of platelets and blood cells.

Abstract: Systems and methods for optical assessments of bioink printability are described. The systems and methods include the use of hardware, software and optical targets to aid in the evaluation of bioink printability. The optical targets are developed and fabricated from 3D printable materials determined to be “nozzle fidelic” and/or materials that possess well characterized thermosensitivity. The optical targets make it possible to rapidly compare and evaluate bioink printability and can be easily customized and tailored for specific applications.

Abstract: A three-dimensional (3D) bioprinting method and system are disclosed. The method includes disposing/immersing a printing platform or surface into a first bioink, such as a bioink resin, curing one or more layer of the first bioink resin onto the printing platform or surface, and removing the printing platform or surface from the first bioink resin. The process is repeated with a second bioink resin such that the second bioink resin is cured on top of the one or more layer of first bioink resin, and can be further repeated with a third or even fourth bioink resin. By varying constituents of one or more or each bioink resin (such as living cell type or polymer), complex, multilayered tissues can be engineered. A system capable of performing the method is also disclosed.

Abstract: A bioprinter (1) comprising at least one printhead (4a, 4b, 4c) with nozzle (5), a printbed (20) onto which the printhead (4a, 4b, 4c) is arranged to print an ink, an ultrasonic sensor arrangement arranged at the printbed (20), a controller (7) arranged to control movement of the printhead/nozzle (4a, 4b, 4c; 5) and to perform calibration of the printhead/nozzle (4a, 4b, 5 4c; 5) based on information from the ultrasonic sensor arrangement.

Abstract: The present invention relates to preparation of bioink composed of cellulose nanofibril hydrogel with native or synthetic Calcium containing particles. The concentration of the calcium containing particles can be between 1% and 40% w/v. Such bioink can be 3D Bioprinted with or without human or animal cells. Coaxial needle can be used where cellulose nanofibril hydrogel filled with Calcium particles can be used as shell and another hydrogel based bioink mixed with cells can be used as core or opposite. Such 3D Bioprinted constructs exhibit high porosity due to shear thinning properties of cellulose nanofibrils which provides excellent printing fidelity. They also have excellent mechanical properties and are easily handled as large constructs for patient-specific bone cavities which need to be repaired. The porosity promotes vascularization which is crucial for oxygen and nutrient supply. The porosity also makes it possible for further recruitment of cells which accelerate bone healing process.

Abstract: The present disclosure relates to a 3D bioprinter (1) comprising a base unit (2). The base unit (2) has a support (3) adapted for mounting of at least one toolhead (4), a communication interface part (5) for communication of data with the at least one toolhead (4), when mounted, and a base unit processing element (7) adapted to communicate with a toolhead processing element (8) of the at least one toolhead over said communication interface part (5). The present disclosure relates further to a 3D bioprinter toolhead. The present disclosure relates further to a method for bioprinting a construct.

Abstract: The present disclosure relates to a 3D printer (1) for 3D printing of a construct. The 3D printer (1) has a print bed (2). The 3D printer further comprises at least one actuating tool head (3) with an extrusion element (4), wherein the extrusion element and the print bed are movable in relation to each other. The 3D printer also comprises at least one sensor (5) arranged to sense a force applied to the print bed (2) by the extrusion element (4), or vice versa. The 3D printer additionally comprises a control element (7) arranged to detect when the sensed force exceeds a predetermined value and to record a position of the print bed or extrusion element related to the detection that the predetermined value is exceeded. The present disclosure also relates to corresponding methods and computer programs.

Abstract: The disclosure relates to a method of culturing and analyzing at least one cell in a microchamber configured to allow for optical inspection of the at least one cell, wherein liquid is extracted from the microchamber for analysis, characterized in that the analysis returns information about particles secreted from the at least one cell and that this information can be correlated to the individual cell and/or cell population. The disclosure further relates to a device for use in the method and a system and a computer program for performing one or more of the steps of the method.

Abstract: The present invention relates to a 3D bioprinted skin tissue model, a method for providing said model and the use of said model. The 3D bioprinted skin tissue model comprises at least one bioink A, at least one cell type A, at least one factor A, wherein the bioink A comprises at least one biopolymer, a thickener, at least one extra-cellular matrix or a decellularized matrix, and optionally a photo initiator and/or cellular additions, the at least one cell type A is an epidermal, dermal and/or hypodermal cell or cell line, and the at least one factor A is a growth factor, protein and/or molecule that stimulates altered or abnormal metabolism of cell type A.

Abstract: Clean chamber technology for 3D printers and bioprinters is described. An airtight chamber or enclosure is provided so that positive pressure can be created inside the chamber. Unfiltered air is sucked in from outside into the chamber through a high efficiency filter such as a HEPA filter, using an electrically powered fan or blower, filtering out at least about 99% of particles and contaminants. The filtered air is then pushed into a 3D printing area inside the chamber and out through vents within the frame of the chamber. The technology provides a clean environment for 3D bioprinting of human tissue models and organs and 3D cell culturing without requiring clean room facilities.

Abstract: A food manufacturing apparatus 100 for manufacturing a food product 1, in particular a meat or meat replacement product 1, in a 3D printing process, includes a movable carrier substrate 10 extending along a longitudinal substrate direction x and being configured for accommodating the food product 1, a deposition device 20 including a plurality of deposition modules 21, which are arranged along the longitudinal substrate direction x of the carrier substrate 10, wherein each deposition module 21 is arranged for depositing components of the food product 1 on the carrier substrate 10, a conveyor device 30 being arranged for moving the carrier substrate 10 along the longitudinal substrate direction x thereof relative to the deposition modules 21, and a collection device 40 being arranged for collecting the food product 1. Furthermore, a method of manufacturing a food product 1, in particular a meat or meat replacement product, is described.

Abstract: Systems and methods for real-time optoelectronic assessments of fluid volume and other fluid properties in fluid dispensing systems are described. The systems and methods include the use of optoelectronic and software algorithms to aid in the determination of fluid volume and other fluid properties, before, during and/or after the fluid dispensing process. The systems and methods described herein allow the fluid dispensing system and its user to have a feedback of real-time data about the dispensing fluid volume and other fluid properties and hence would improve the control, speed and quality of the fluid dispensing system and its dispensed fluid.

Abstract: The present invention provides for a liver tissue model construct composed of biomaterials and cells, to be used for scientific research within in the 3D liver tissue modelling field. The applications of said tissue model construct can be specific for pharmaceutical evaluations and/or discoveries, regenerative medicine investigations, tissue engineering developments, and liver physiology and/or pathology.

Abstract: The present disclosure relates to the field of liquid handling and dispensing systems in combination with additive manufacturing. In particular, it relates to temperature-controlled units, i.e. dispensing heads and source well holders, for receiving, holding and releasing liquid and semi-liquid material, liquid-handling and dispensing systems, apparatuses and methods for applying temperature-sensitive liquids. A temperature-controlled unit (1) comprises at least one Peltier element (3), each Peltier element having opposite first and second surfaces (4a, 4b). The unit (1) further comprises at least one cooling element (5). The at least one Peltier element (3) is arranged to have each respective first surface (4a) facing a reservoir block (2) of the unit (1). The at least one cooling element (5) is thermally connected to the Peltier element (3) and arranged to transfer heat generated by the at least one Peltier element (3) and dissipate the transferred heat away from the at least one Peltier element (3).

Abstract: A three-dimensional (3D) bioprinting method and system are disclosed. The method includes disposing/immersing a printing platform or surface into a first bioink, such as a bioink resin, curing one or more layer of the first bioink resin onto the printing platform or surface, and removing the printing platform or surface from the first bioink resin. The process is repeated with a second bioink resin such that the second bioink resin is cured on top of the one or more layer of first bioink resin, and can be further repeated with a third or even fourth bioink resin. By varying constituents of one or more or each bioink resin (such as living cell type or polymer), complex, multilayered tissues can be engineered. A system capable of performing the method is also disclosed.

Abstract: The present invention relates to a cell monitoring system for automatic cell monitoring and to a compact fluorescence microscope for high resolution live-cell imaging. The microscope comprises light sources (12), light source lenses (11), excitation filters (10), and beam combining means (9). The detector unit comprises emission filter (4), tube lens (5), and a detector (7) and optionally beam folding elements (6) for compactness. A dielectric mirror (3) combines and divides the excitation and the emission light.

Abstract: A material cartridge arrangement (100, 101) for a dispensing system, comprising a material cartridge (2) having a first end (2a), an opposing second end (2b) and an ink material channel (5) extending between the first and second ends in the material cartridge (2), the ink material channel (5) being bounded by an ink material channel wall/walls (9) extending between the first (2a) and second ends (2b). An ink material pressurising device (3) is arranged to cause ink material hold in the ink material channel (5) to flow in a direction from said first end (2a) towards said second end (2b) and through an ink material outlet. At least one temperature sensing element (16) is arranged at a position along the direction of extension of the ink material channel (5) and arranged to measure a temperature at the ink material channel wall (9).