Abstract: Methods, systems, and compositions are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation, and for obtaining desired cells from cryopreserved and fresh bone marrow. In some embodiments, the methods provide cryopreserved compositions having increased viability of bone marrow cells.

Abstract: The present disclosure provides composition and methods preparing a population of isolated MSCs for pharmaceutical application, the method comprising: providing a population of isolated MSCs; and synchronizing the population of isolated MSCs, wherein no more than 25% of the population of isolated MSCs are in the S phase of the cell cycle.

Abstract: The present disclosure relates to, at least, a vacuum-assisted method for infiltrating cadaver bone with a cryoprotectant and a method for rapidly warming the cryopreserved cadaver bone for bone marrow processing.

Abstract: Described herein are methods of cell therapies. Also described herein are methods of generating donor derived T cells in an organ transplant recipient, by administering bone marrow stem cells to the organ transplant recipient about 1 to about 30 days after the organ transplant recipient receives one or more organ transplants.

Abstract: Methods, systems, and compositions are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation, and for obtaining desired cells from cryopreserved and fresh bone marrow.

Abstract: Described herein are methods of cell therapies. Also described herein are methods of generating donor derived T cells in an organ transplant recipient, by administering bone marrow stem cells to the organ transplant recipient about 1 to about 30 days after the organ transplant recipient receives one or more organ transplants.

Abstract: A bone cutting assembly includes a manually actuated upper cutting element that carries a plurality of cutting blades configured to cut through frozen bone segments. A lower cutting element supports a bone segment to be cut and can include cutting blades aligned with the cutting blades of the upper cutting assembly. A pivoting linkage or a rack and pinion arrangement can be provided between the upper cutting element and a manually operated handle to provide sufficient mechanical advantage or leverage to allow an operator to manually cut through the bone.

Abstract: A bone cutting assembly includes a manually actuated upper cutting element that carries a plurality of cutting blades configured to cut through frozen bone segments. A lower cutting element supports a bone segment to be cut and can include cutting blades aligned with the cutting blades of the upper cutting assembly. A pivoting linkage or a rack and pinion arrangement can be provided between the upper cutting element and a manually operated handle to provide sufficient mechanical advantage or leverage to allow an operator to manually cut through the bone.

Abstract: Disclosed is a device useful for preserving tissue samples after extraction from human or animal patients. The device includes a circular blade for taking a tissue sample, the blade being coupled to a sealing cap which mates to one end of a container. A retrieval port is also disclosed for extracting fluids from the container. Methods are also disclosed by which tissue samples may be taken and prepared for storage, processing, culture, or other analysis.

Abstract: A bone cutting assembly includes a manually actuated upper cutting element that carries a plurality of cutting blades configured to cut through frozen bone segments. A lower cutting element supports a bone segment to be cut and can include cutting blades aligned with the cutting blades of the upper cutting assembly. A pivoting linkage or a rack and pinion arrangement can be provided between the upper cutting element and a manually operated handle to provide sufficient mechanical advantage or leverage to allow an operator to manually cut through the bone.

Abstract: Disclosed are viable cell compositions and related methods of preparation, maintenance and use. The viable cell composition can contain specified levels of cells, hydroxyethyl starch, and dimethylsulfoxide, and can be cryopreserved. The cryopreserved form of the composition can be thawed and combined with an aqueous liquid diluting medium to prepare a diluted viable cell composition that can contain specified, reduced levels of the dimethylsulfoxide and hydroxyethyl starch. The diluting medium can contain trehalose. The prepared, diluted viable cell composition can be administered to a patient.

Abstract: The present disclosure provides a composition comprising a bioactive fraction derived from a platelet concentrate, methods of making the bioactive fraction, and culture medium supplemented with the bioactive fraction. Preferred bioactive fractions have relatively low fibrinogen concentrations while retaining native growth factors in beneficial amounts and ratios.

Abstract: Disclosed is a device useful for preserving tissue samples after extraction from human or animal patients. The device includes a circular blade for taking a tissue sample, the blade being coupled to a sealing cap which mates to one end of a container. A retrieval port is also disclosed for extracting fluids from the container. Methods are also disclosed by which tissue samples may be taken and prepared for storage, processing, culture, or other analysis.

Abstract: Disclosed is a device useful for preserving tissue samples after extraction from human or animal patients. The device includes a circular blade for taking a tissue sample, the blade being coupled to a sealing cap which mates to one end of a container. A retrieval port is also disclosed for extracting fluids from the container. Methods are also disclosed by which tissue samples may be taken and prepared for storage, processing, culture, or other analysis.

Abstract: The present disclosure provides a composition comprising a bioactive fraction derived from a platelet concentrate, methods of making the bioactive fraction, and culture medium supplemented with the bioactive fraction. Preferred bioactive fractions have relatively low fibrinogen concentrations while retaining native growth factors in beneficial amounts and ratios.

Abstract: Disclosed is a device useful for preserving tissue samples after extraction from human or animal patients. The device includes a circular blade for taking a tissue sample, the blade being coupled to a sealing cap which mates to one end of a container. A retrieval port is also disclosed for extracting fluids from the container. Methods are also disclosed by which tissue samples may be taken and prepared for storage, processing, culture, or other analysis.

Abstract: Compositions and methods for culturing therapeutic cells are provided herein. According to at least one embodiment, compositions comprising cord blood plasma and lysed platelets and methods for making and using same are provided herein.

Abstract: A method is disclosed for removal of cryoprotectants from preserved suspension of biological cells and tissues. Sorbent materials are used, alone or in combination, to bind the cryoprotectant component of preserved cell suspensions with minimal osmotic stress on the preserved cells. The present method is used to effectively remove the cryoprotectants from cryopreserved cells and tissues prior to their use in transfusion, transplantation, insemination or other applications, with minimal osmotic damage due to cell swelling. Specific devices and methods are described.

Abstract: A method to remove cryoprotectants from cryopreserved biological cells and tissues is described. Enzymes are used to convert compounds used as cryoprotectants for cells during cryopreservation to membrane impermeable products. This process effectively removes the cryoprotectant chemicals from the cells prior to their use in transfusion, transplantation, insemination or other applications, without causing osmotic damage due to cell swelling. Methods are described for subsequent removal of the enzymes and enzyme conversion products from the cell and tissue preparations.