Abstract: An example system includes a first gas-turbine engine configured to propel an aircraft, the first gas-turbine engine comprising a first electric starter, the first electric starter configured to rotate a spool of the first gas-turbine engine; and one or more controllers collectively configured to: cause, following operation of the first gas-turbine engine, the first electric starter to perform barring of the first gas-turbine engine; measure, during the barring of the first gas-turbine engine, values of one or more parameters of the first gas-turbine engine; and determine, based on the values of the one or more parameters, whether the first electric starter is available for use in performing mid-air restart of the first gas-turbine engine.

Abstract: The present disclosure relates to Neisseria meningitidis (Nme) 2 Cas9 (Nme2Cas9) and Nme2SmuCas9 variants comprising one or more amino acid substitutions with increased genome editing activities (e.g., improve nuclease and base editing efficiencies).

Abstract: The present invention is related to the field of gene editing. In particular, the gene editing is directed toward single nucleotide base editing. For example, such single nucleotide base editing results in a conversion of a mutated base pair to a wild type base pair. The high accuracy and precision of the presently disclosed single nucleotide base gene editor is accomplished by a fusion protein including an NmeCas9 nuclease and an inlaid nucleotide deaminase protein domain. The Nme2Cas9 protospacer interacting domain may be replaced with an SmuCas9 protospacer interacting domain.

Abstract: A non-pressurized fluid reservoir for a vehicle coolant system includes a bottle configured to store fluid. The bottle has a neck defining an inner circumferential surface that is devoid of threads. The reservoir further includes a cap having a lid and a shank extending from the lid. The shank has an outer circumferential surface and with a spiral ramp supported on the outer circumferential surface to encircle a portion of the shank. The shank is receivable within the neck with the spiral ramp adjacent to the inner circumferential surface to mitigate fluid leaks through the neck.

Abstract: One variation of a system for an electric motor includes a set of coil assemblies defining: an inner radial facet, an outer radial facet, a first axial facet, and a second axial facet opposite the first axial facet. Additionally, each coil assembly in the set of coil assemblies includes a receiving member arranged at the outer radial facet of the coil assembly. Furthermore, the system includes a rotor comprising a set of magnetic elements: encompassing the inner radial facet, the outer radial facet, the first axial facet, and the second axial facet of the set of coil assemblies; and defining a radial magnetic tunnel. The system also includes a housing: engaging the receiving member of each coil assembly, in the set of coil assemblies to couple the housing to the set of coil assemblies; and includes a shaft coupled to the set of magnetic elements.

Abstract: Techniques for transferring data to a secure computing region that is isolated from any public networks is disclosed. In some embodiments, one or more artifacts for the secure computing region are packaged. As part of the packaging, one or more data packets and metadata for the one or more artifacts can be generated. The metadata indicates corresponding destination components for the one or more artifacts within the secure computing region. The data packet(s) are received and transmitted by a first data diode endpoint device. The first data diode endpoint device is configured to transmit data to the secure computing region via a second data diode endpoint device, the second data diode endpoint device being configured to restrict data from being transmitted to a destination outside of the secure computing region. The transmission of the one or more data packets may be monitored by the system.

Abstract: Methods and apparatus consistent with the invention provide the ability to organize and build understandings of machine data generated by a variety of information-processing environments. Machine data is a product of information-processing systems (e.g., activity logs, configuration files, messages, database records) and represents the evidence of particular events that have taken place and been recorded in raw data format. In one embodiment, machine data is turned into a machine data web by organizing machine data into events and then linking events together.

Abstract: The disclosure provides methods and compositions for gene editing. In particular. the disclosure relates to compositions and methods of making and using modified nucleic acid donor templates for highly efficient and precise gene editing, such as gen editing in immune cells.

Abstract: A display system includes a head-mounted display configured to project light, having different amounts of wavefront divergence, to an eye of a user to display virtual image content appearing to be disposed at different depth planes. The wavefront divergence may be changed in discrete steps, with the change in steps being triggered based upon whether the user is fixating on a particular depth plane. The display system may be calibrated for switching depth planes for a main user. Upon determining that a guest user is utilizing the system, rather than undergoing a full calibration, the display system may be configured to switch depth planes based on a rough determination of the virtual content that the user is looking at. The virtual content may have an associated depth plane and the display system may be configured to switch to the depth plane of that virtual content.

Abstract: In a general aspect, an optimization problem is solved using a hybrid computing system. A classical processor unit receives a first data structure that represents the optimization problem. The classical processor unit executes a branch-and-bound process on the first data structure to generate values for a first subset of elements of a solution to the optimization problem. A second data structure is generated based on the first data structure and the first subset of elements. The second data structure represents a reduced version of the optimization problem. A quantum processor unit and a classical processor unit are used to execute a quantum approximate optimization algorithm (QAOA) on the second data structure to generate values for a second subset of the elements of the solution to the optimization problem. The first subset and second subset are combined to obtain the solution to the optimization problem.

Abstract: In examples, a clutch assembly includes a first clutch member disposed near a first end of a first shaft. The first clutch member defines a first surface. The first shaft includes a starter motor coupled to a second end of the first shaft. The clutch assembly includes a second clutch member disposed near a first end of a second shaft. The second clutch member defines a second surface opposing the first surface, and the second shaft includes an accessory gearbox of a gas turbine engine coupled to a second end of the second shaft. The surfaces of the first and second clutch member engage such that rotational motion is transferred between the first clutch member and the second clutch member. The first clutch member and the second clutch member are configured to passively disengage from each other and actively reengage with each other.

Abstract: Systems and methods for building passive and active electronics with diamond-like carbon (DLC) coatings are provided herein. DLC may be layered upon substrates to form various components of electronic devices. Passive components such as resistors, capacitors, and inductors may be built using DLC as a dielectric or as an insulating layer. Active components such as diodes and transistors may be built with the DLC acting substantially like a semiconductor. The amount of sp2and sp3 bonded carbon atoms may be varied to modify the properties of the DLC for various electronic components.

Abstract: A balloon garland assembly includes a frame assembly having a strut and a connector for coupling the strut to another strut. A socket is formed in the strut, the socket having an aperture formed through the strut and a gasket fit in the aperture. A balloon assembly is fit into the gasket, the balloon assembly comprising a balloon, an expander in a neck of the balloon, and a stem extending out from the expander away from the balloon. The stem is configured to fit into the gasket with a gas-impermeable fit.

Abstract: Extended Nucleic Acid (exNA)-modified crRNAs and tracrRNAs are provided. Methods of using the crRNAs and tracrRNAs for genome editing with a CRISPR nuclease and kits for performing the same are also provided.

Abstract: The disclosure provides novel methods and compositions for gene editing. In particular, the disclosure relates to compositions and methods of making modified nucleic acid donor templates for highly efficient and precise gene editing.