Abstract: Provided herein are methods, compositions, and kits to spatially detect genetic variants in a target nucleic acid via templated-ligation and reversible terminator nucleotides.

Abstract: Provided herein are methods, compositions, and kits for reducing capture of analytes from a biological sample on a spatial array on areas not covered by a biological sample.

Abstract: The present disclosure relates to compositions, methods, and kits for generating capture probes on a substrate for identifying the location of analytes in a biological sample.

Abstract: Systems and methods for tissue classification are provided. An image of tissue on a substrate is obtained as a plurality of pixels. Fiducial markers are on the substrate boundary. Pixels are assigned to a first class, indicating tissue sample, or a second class, indicating background. The assigning uses the fiducial markers to define a bounding box within the image and disregards pixels outside the box. Then, heuristic classifiers are applied to the pixels: for each respective pixel in the plurality of pixels, each heuristic classifier votes for the respective pixel between the first and second class, thereby forming an aggregated score for each pixel that in one of first class, likely first class, likely second class, and obvious second class. The aggregated score and intensity of each pixel is applied to a segmentation algorithm to assign a probability to each pixel of being tissue sample or background.

Abstract: Systems and methods for tissue classification are provided. An image of tissue on a substrate is obtained as a plurality of pixels. Fiducial markers are on the substrate boundary. Pixels are assigned to a first class, indicating tissue sample, or a second class, indicating background. The assigning uses the fiducial markers to define a bounding box within the image and disregards pixels outside the box. Then, heuristic classifiers are applied to the pixels: for each respective pixel in the plurality of pixels, each heuristic classifier votes for the respective pixel between the first and second class, thereby forming an aggregated score for each pixel that in one of first class, likely first class, likely second class, and obvious second class. The aggregated score and intensity of each pixel is applied to a segmentation algorithm to assign a probability to each pixel of being tissue sample or background.

Abstract: Provided herein are methods, compositions and kits for detecting SARS-CoV-2 and host nucleic acids in a tissue sample.

Abstract: Provided herein are methods for selective silencing of one or more probes used in templated ligation, or RNA-templated ligation. In some embodiments, the selective silencing is achieved using one or more blocker oligonucleotides.

Abstract: The present disclosure relates to compositions and methods for generating capture probes on a substrate for identifying the location of analytes in a biological sample.

Abstract: The present disclosure relates to compositions, methods, and kits for generating capture probes on a substrate for identifying the location of analytes in a biological sample.

Abstract: The present disclosure relates to compositions and methods for generating capture probes on a substrate for identifying the location of analytes in a biological sample.

Abstract: The present disclosure relates to compositions and methods for generating capture probes on a substrate for identifying the location of analytes in a biological sample.

Abstract: Systems and methods for spatial analysis of analytes include placing a sample on a substrate having fiducial markers and capture spots. Then, an image of the sample is acquired and sequence reads are obtained from the capture spots. Each capture probe plurality in a set of capture probe pluralities is (i) at a different capture spot, (ii) directly or indirectly associates with analytes from the sample and (iii) has a unique spatial barcode. The sequencing reads serve to detect the analytes. Sequencing reads include a spatial barcode of the corresponding capture probe plurality. Spatial barcodes localize reads to corresponding capture spots, thereby dividing them into subsets, each subset for a respective capture spot. Fiducial markers facilitate a composite representation comprising (i) the image aligned to the capture spots and (ii) a representation of each subset of sequence reads at respective positions within the image mapping to the corresponding capture spots.

Abstract: Systems and methods for tissue classification are provided. An image of tissue on a substrate is obtained as a plurality of pixels. Fiducial markers are on the substrate boundary. Pixels are assigned to a first class, indicating tissue sample, or a second class, indicating background. The assigning uses the fiducial markers to define a bounding box within the image and disregards pixels outside the box. Then, heuristic classifiers are applied to the pixels: for each respective pixel in the plurality of pixels, each heuristic classifier votes for the respective pixel between the first and second class, thereby forming an aggregated score for each pixel that in one of first class, likely first class, likely second class, and obvious second class. The aggregated score and intensity of each pixel is applied to a segmentation algorithm to assign a probability to each pixel of being tissue sample or background.

Abstract: A flat panel antenna for satellite communications, the flat panel antenna including a printed circuit board (PCB) including a plurality of patches and a feed network; a waveguide manifold including a transmit feed network on one side and a receive feed network on the opposite side; a transition region on the transmit feed network wherein a waveguide input port transitions to a ridged waveguide for compact dimension; a transition region on the transmit feed network wherein the waveguide input port transitions to the ridged waveguide for compact dimension; an integrated transmit reject filter on the receive feed network; and wherein the flat panel antenna is operable to provide right hand circular polarization TX and left-hand circular polarization RX.

Abstract: A retrofit electric drive apparatus is provided for converting a fossil fuel fitted motorcycle into an electric-powered motorcycle. In an embodiment, the apparatus includes a housing supporting electric drive train components. At least a portion of the housing is shaped to congruously match a portion of the motorcycle frame and reside within an envelope defined by the motorcycle frame. In another embodiment, the retrofit electric drive apparatus comprises a housing including a first side element and a second side element, the first side element connected to the second side element at a predetermined spacing to create an internal space. The housing is shaped to reside within an envelope defined by the motorcycle frame and has at least a portion congruous with a portion of the motorcycle frame defining the envelope. In another embodiment, the retrofit electric drive apparatus comprises a housing kit.

Abstract: A saltwater fishing assembly includes a body resembling a forage fish. The body includes a quick connect device that is swivelable relative to a fishing line and is reelable to a fishing rod tip. A quick connection subassembly is connectable to the quick connect device of the body and includes a hook and is releasable with a hooked fish. Methods of using the fishing assembly are also provided.

Abstract: A saltwater fishing assembly includes a body resembling a forage fish. The body includes a quick connect device that is swivelable relative to a fishing line and is reelable to a fishing rod tip. A quick connection subassembly is connectable to the quick connect device of the body and includes a hook and is releasable with a hooked fish. Methods of using the fishing assembly are also provided.

Abstract: A hip prosthesis has an oblong plate with at one head end a mounting pin provided with a hinge ball, and at the opposite head end a fastening pin for fastening the hip prosthesis in a shaft of a bone. A first part of the plate extends on a first side of the mounting pin. The mounting pin forms an angle ? smaller than 90 degrees with the first part of the plate and the tangent to the fastening pin at the location of the connection between the fastening pin and the first part of the plate forms an angle ? greater than 90 degrees with the first part of the plate. In order to insert the hip prosthesis into a bone in a simple manner, the fastening pin includes a curved shape from the plate onwards in a direction to the first part of the plate.

Abstract: A saltwater fishing assembly including a body resembling a forage fish. The body includes a quick connect device that is swivelable relative to a fishing line and is reelable to a fishing rod tip. A quick connection subassembly is connectable to the quick connect device of the body and includes a hook and is releasable with a hooked fish. Methods of using the fishing assembly are also provided.

Abstract: A saltwater fishing assembly includes a body resembling a forage fish. The body includes a quick connect device that is swivelable relative to a fishing line and is reelable to a fishing rod tip. A quick connection subassembly is connectable to the quick connect device of the body and includes a hook and is releasable with a hooked fish. Methods of using the fishing assembly are also provided.