Abstract: The present invention relates to temperature-sensitive variants of a Class-II Cas9 protein, polynucleotides encoding said variants, nucleic acid constructs and expression vectors comprising polynucleotides encoding said variants, host cells expressing said variant, methods of transient repression and expression of one or more genome target sequence using said variants, and use of said variants, polynucleotides, nucleic acid constructs, expression vectors, host cells, and methods.

Abstract: The present invention relates to protease variants, polynucleotides encoding said variants, nucleic acid constructs and expression vectors comprising said polynucleotides, host cells expressing said variants, methods of obtaining the variants, detergent compositions comprising said variants, and use of said variants or said detergent compositions.

Abstract: A needle cannula for an injection device, extends along a central axis and comprises: a) a skin piercing distal end (133b), b) a proximal end (133a) configured for penetrating a seal structure (102b) of a medicament container (102), and c) a sidewall extending between the skin piercing distal end (133b) and the proximal end (133a), wherein the proximal end (133a) is defined by an angled surface (134) pointing proximally, the angled surface (134) defining a leading portion (134.1) and a heel portion (134.2), and wherein the angled surface (134) comprises a surface portion (134a, 134b) at the heel portion (134.2) forming an angle (?1) between 50 to 75 degrees with respect to the central axis, and further comprises a surface portion (134c) at the leading portion (134.1) forming an angle (?2) between 60 to 85 degrees with respect to the central axis. An injection needle assembly for an injection device and an injection device comprising such assembly are further described.

Abstract: Disclosed are protease inhibitors capable of inhibiting the protease activity of a S1 or a S8 protease at ambient pH, but where the inhibitor further has a pH dependent binding to the S1 or the S8 inhibitor meaning that a complex of a S1 and a S8 protease and the inhibitor dissociates when pH is lowered to a pH below 6.0, e.g. to a pH value in the range of 4.0 to 6.0; e.g. in the range of 4.5 to 6.0; e.g. in the range of 4.5 to 5.5; whereby the protease activity is released.

Abstract: The present invention relates to a method for counter-selection by inhibition conditionally essential genes.

Abstract: The present invention relates to temperature-sensitive variants of a Class-II Cas9 protein, polynucleotides encoding said variants, nucleic acid constructs and expression vectors comprising polynucleotides encoding said variants, host cells expressing said variant, methods of transient repression and expression of one or more genome target sequence using said variants, and use of said variants, polynucleotides, nucleic acid constructs, expression vectors, host cells, and methods.

Abstract: The present invention relates to a method for constructing a host cell expressing a polypeptide of interest from at least two ORF's stably integrated onto the chromosome of the host cell comprising the steps of: a) providing the at least two ORF's encoding the same polypeptide, wherein the at least two DNA sequences of the ORF's differ in at least one position; b) integrating the at least two ORF's in the same orientation on the host cell chromosome.

Abstract: A needle cannula for an injection device, extends along a central axis and comprises: a) a skin piercing distal end (133b), b) a proximal end (133a) configured for penetrating a seal structure (102b) of a medicament container (102), and c) a sidewall extending between the skin piercing distal end (133b) and the proximal end (133a), wherein the proximal end (133a) is defined by an angled surface (134) pointing proximally, the angled surface (134) defining a leading portion (134.1) and a heel portion (134.2), and wherein the angled surface (134) comprises a surface portion (134a, 134b) at the heel portion (134.2) forming an angle (?1) between 50 to 75 degrees with respect to the central axis, and further comprises a surface portion (134c) at the leading portion (134.1) forming an angle (?2) between 60 to 85 degrees with respect to the central axis. An injection needle assembly for an injection device and an injection device comprising such assembly are further described.

Abstract: The present invention relates to methods for improving at least one property of a microorganism host cell by repressing the expression of one or more genome target sequence of interest by CRISPR-inhibition as well as the resulting host cells and methods of production emplying said host cells.

Abstract: The present invention relates to enzyme variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; methods of using the variants and composition comprising variants.

Abstract: A method for purifying a protein product prepared in a fermentation process where the protein of interest is present in solid, crystalline or amorphous form in the fermentation container is disclosed, where the pH of the fermentation broth is adjusted to a low pH whereby the protein of interest dissolves and can be efficiently separated from insoluble.

Abstract: The present invention relates to a method of producing a natively non-secreted polypeptide without a secretion signal in a Bacillus host cell and recovering the polypeptide without performing a lysis step as well as to a Bacillus host cell comprising one or more exogenous or heterologous polynucleotides encoding a natively non-secreted polypeptide with no secretion signal.

Abstract: In a method for applying a curable liquid using an inkjet printing method, a gloss level is controlled by first applying a first layer of liquid and curing the first layer. Having thus obtained a layer having known properties, a second layer is applied on the first layer. Droplets of the second layer are uncured for a predetermined period of time allowing the droplets to spread and coalesce to form a layer having a smooth and flat surface such to provide a desired gloss level, in particular a high gloss. Further, a printing apparatus configured to perform the method is provided.

Abstract: In a method for applying a curable liquid using an inkjet printing method, a gloss level is controlled by first applying a first layer of liquid and curing the first layer. Having thus obtained a layer having known properties, a second layer is applied on the first layer. Droplets of the second layer are uncured for a predetermined period of time allowing the droplets to spread and coalesce to form a layer having a smooth and flat surface such to provide a desired gloss level, in particular a high gloss. Further, a printing apparatus configured to perform the method is provided.

Abstract: Power amplification devices are described, which are configured to amplify a radio frequency (RF) transmission signal. The power amplification device includes a voltage regulation circuit and a power amplification circuit. The voltage regulation circuit is configured to generate a regulated voltage for the power amplification device from a supply voltage and to adjust a regulated voltage level of the regulated voltage in accordance with a voltage adjustment gain. The voltage adjustment gain of the voltage regulation circuit is set by a feedback resistance. To help prevent the voltage regulation circuit from saturating, the voltage regulation circuit adjusts the feedback resistance to reduce the voltage regulation gain.

Abstract: The invention provides methods of screening a microbial host cell for a property of interest in a microfluidic device, the method comprising the steps of: a) transforming a d-alanine racemase-deficient microbial host cell with a polynucleotide construct comprising: i) one or more polynucleotide region providing the property of interest when present in the host cell, and ii) at least one polynucleotide region complementing the d-alanine racemase deficiency when present in the host cell; and b) screening the transformed host cell for the property of interest in the microfluidic device in the absence of externally provided d-alanine.

Abstract: A mouse hole damper device positioned at the bottom portion of a mouse hole pipe, the mouse hole damper being arranged to dampen an impact from an object falling in the mouse hole pipe, and the mouse hole damper including a material which has been worked into forming walls around elongated openings, and the material being arranged in such a way that the openings are substantially parallel to the longitudinal axis of the mouse hole pipe.

Abstract: A power amplification device is disclosed that includes a power amplification circuit operable to amplify a radio frequency (RF) signal in accordance with an amplification gain, and a voltage regulation circuit operable to generate a regulated voltage. A regulated voltage level of the regulated voltage sets the amplification gain. To help prevent the voltage regulation circuit from saturating, the voltage regulation circuit is configured to reduce a voltage adjustment gain when the regulated voltage level reaches a threshold voltage level. In one embodiment, the threshold voltage level is set to be higher when a band-select signal indicates that the RF signal is being transmitted within a first frequency band, and is set to be lower when the band-select signal indicates that the RF signal is being transmitted within a second frequency band. The spectral performance of the power amplification device thus improves with regard to the second frequency band.

Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.

Abstract: The present invention relates to a method for constructing a host cell expressing a polypeptide of interest from at least two ORF's stably integrated onto the chromosome of the host cell comprising the steps of: a) providing the at least two ORF's encoding the same polypeptide, wherein the at least two DNA sequences of the ORF's differ in at least one position; b) integrating the at least two ORF's in the same orientation on the host cell chromosome.