Abstract: The present invention relates to temperature-sensitive variants of a Class-II Cas9 protein, polynucleotides encoding said variants, nucleic acid constructs and expression vectors comprising polynucleotides encoding said variants, host cells expressing said variant, methods of transient repression and expression of one or more genome target sequence using said variants, and use of said variants, polynucleotides, nucleic acid constructs, expression vectors, host cells, and methods.

Abstract: A dynamic fluid flow control structure is provided that allows precise control over fluid flow using a series of two or more orifices, at least one of which may be reconfigured to change its flow characteristics. A flood control system and a flood control process are provided that emulate a preset discharge profile over time. Some versions of the structure, process, and system can be used to provide controlled storm discharge patterns in a developed area that emulate the natural pre-development discharge patterns.

Abstract: The present invention is directed at a thermal expansion valve for a residential refrigeration application, including a valve body with a high pressure inlet, a low pressure outlet, a first suction gas port and a second suction gas port. The valve further includes a superheat defining mechanism with a diaphragm, a pushpin and a charge.

Abstract: The invention relates to a capsule for a valve as well as to a valve comprising such a capsule. The task of the present invention is to provide a valve in which tight tolerances for construction can be achieved and the assembly of the valve is simplified. According to the present invention the above task is solved by a capsule (1) for a valve comprising at least one diaphragm (2), a valve seat (3), a capsule inlet (5) and a capsule outlet (6). The valve seat (3) is openable and closable to permit or stop a fluid flow from the capsule inlet (5) to the capsule outlet (6). The above task is also solved by a capsule (1) for a valve that comprises at least one diaphragm (2), a valve seat (3), a capsule inlet (5) and a capsule outlet (6), wherein the capsule (1) is structured and arranged to control the position of a pilot valve element external to the capsule (1).

Abstract: A dynamic fluid flow control structure is provided that allows precise control over fluid flow using a series of two or more orifices, at least one of which may be reconfigured to change its flow characteristics. A flood control system and a flood control process are provided that emulate a preset discharge profile over time. Some versions of the structure, process, and system can be used to provide controlled storm discharge patterns in a developed area that emulate the natural pre-development discharge patterns.

Abstract: A mining system with an inertial guidance system configured to enable precise excavation of geological material without a need to advance a survey line over a long distance and/or nonlinear excavation path, thereby maximizing productivity of the mind by minimizing a width of un-mined material necessary for support between adjacent excavation paths and minimizing equipment downtime.

Abstract: A mining system with an inertial guidance system configured to enable precise excavation of geological material without a need to advance a survey line over a long distance and/or nonlinear excavation path, thereby maximizing productivity of the mind by minimizing a width of un-mined material necessary for support between adjacent excavation paths and minimizing equipment downtime.

Abstract: A tool for cutting a substrate and method of use. The tool has a blade with a distal cutting tip at least one shoulder on one side of and proximal the cutting tip. The cutting tip and shoulder are positioned so that when cutting, at least a portion of the at least one shoulder is in contact with at least one cut edge of the substrate defined by a cut line formed by penetrating the substrate thickness with the cutting tip. A heater heats the blade shoulder to a temperature that causes the portion of the shoulder in contact with the cut edge of the substrate to seal the cut edge. The method includes heating the blade, penetrating the substrate with the cutting tip with a portion of the shoulder contacting the substrate, and moving the blade relative to the substrate.

Abstract: A method of making a self-expanding stent entails applying a bend stress sufficient to over-bend a portion of a wire by an amount in a range from about 85% to about 105%, where the wire comprises a Ni—Ti alloy and includes from about 40% to about 46% cold work. The bend stress is then released, thereby forming a bend having a predetermined bend angle in the wire. The application and release of the bend stress are repeated on successive portions of the wire in order to create a series of bends along a length of the wire in a predetermined bend pattern. The wire comprising the predetermined bend pattern is then positioned about a mandrel in an expanded stent geometry and heat set. Thus, a self-expanding stent is formed.

Abstract: A tool for cutting a substrate and method of use. The tool has a blade with a distal cutting tip at least one shoulder on one side of and proximal the cutting tip. The cutting tip and shoulder are positioned so that when cutting, at least a portion of the at least one shoulder is in contact with at least one cut edge of the substrate defined by a cut line formed by penetrating the substrate thickness with the cutting tip. A heater heats the blade shoulder to a temperature that causes the portion of the shoulder in contact with the cut edge of the substrate to seal the cut edge. The method includes heating the blade, penetrating the substrate with the cutting tip with a portion of the shoulder contacting the substrate, and moving the blade relative to the substrate.

Abstract: A mining system with an inertial guidance system configured to enable precise excavation of geological material without a need to advance a survey line over a long distance and/or nonlinear excavation path, thereby maximizing productivity of the mind by minimizing a width of un-mined material necessary for support between adjacent excavation paths and minimizing equipment downtime.

Abstract: A mining system with an inertial guidance system configured to enable precise excavation of geological material without a need to advance a survey line over a long distance and/or nonlinear excavation path, thereby maximizing productivity of the mind by minimizing a width of un-mined material necessary for support between adjacent excavation paths and minimizing equipment downtime.

Abstract: A valve seat for hydraulic fracturing valves employs a through-hardening process, for example, with austempering, to provide greater wear resistance at the interface between the valve seat and the valve plug as well as resistance to abrasion and leakage in the interface between an alignment ring of the valve seat and a bore in the pump body.

Abstract: A dynamic fluid flow control structure is provided that allows precise control over fluid flow using a series of two or more orifices, at least one of which may be reconfigured to change its flow characteristics. A flood control system and a flood control process are provided that emulate a preset discharge profile over time. Some versions of the structure, process, and system can be used to provide controlled storm discharge patterns in a developed area that emulate the natural pre-development discharge patterns.

Abstract: A valve seat puller provides jaws for expanding on a first side of a valve seat to engage that seat in a first reaction for removal of the seat in a second direction.

Abstract: A bulb (5) for a thermostatic expansion valve is provided, said bulb (5) comprising a chamber (7), said chamber (7) being located within a metallic casing of said bulb and being filled with a filling adapted to influence a valve element of said thermostatic expansion valve. Service of a temperature controlled valve connected to a bulb should be facilitated. To this end said bulb (5) comprises a connection geometry (10) adapted to be connected to a capillary member (6) and said casing being provided with a closed opening zone located within said connection geometry (10), said opening zone being adapted to be opened upon mounting a counterpart (15) to said connection geometry (10).

Abstract: The invention relates to an insert (1) for a thermostatic expansion valve as a thermostatic expansion valve (2) comprising such an insert and to a method of assembling such a thermostatic expansion valve (2). The insert (1) comprises an expansion valve element (3) and an expansion valve seat (4). The expansion valve element (3) is held inside the insert (1) by a biasing member (5), wherein the insert (1) is structured and arranged to be inserted into a valve housing (8). The task of the invention is to provide an insert for a thermostatic expansion valve that allows a simple assembly of the valve and also provides an improved functionality. The task is solved in that the insert (1) further comprises a control valve (12), wherein the control valve (12) is located in the same flow path through the insert (1) as the expansion valve seat (4).