Patents by Inventor Erika Leonard

Erika Leonard has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Immunofluorescence assays
    Patent number: 11885797
    Abstract: Provided herein are immunofluorescence assays that do not use an intensely colored lipophilic dye, and that result in a significant reduction of fluorescence due to aldehyde fixation, collagen, elastin, and/or red blood cells. In certain embodiments of the present invention, a two-step treatment of the tissue is contemplated that leads to the incorporation of reduced heteropoly acids or salts thereof (e.g., phosphomolybdate species) in the tissue. This reagent binds tightly to hydrophilic regions of the tissue and effectively quenches the fluorescence in those regions. In doing so, the overall signal-to-noise ratio for the immunofluorescence assay is increased.
    Type: Grant
    Filed: April 5, 2021
    Date of Patent: January 30, 2024
    Assignee: VECTOR LABORATORIES, INC.
    Inventors: Timothy B. Karpishin, Pamela James, Erika Leonard
  • COMPOSITIONS AND METHODS FOR REMOVAL OF BOUND DETECTION AGENTS
    Publication number: 20230358649
    Abstract: Disclosed are compositions, methods, systems, and kits for the detection of markers of interest, which may allow for sequential multiplexing. In some cases, the methods comprise sequential staining of biological samples with detection sets and removal of bound detection agents which may allow for sequential staining with additional detection agents.
    Type: Application
    Filed: May 8, 2023
    Publication date: November 9, 2023
    Inventors: August ESTABROOK, Dennis HICKS, Pamela JAMES, Timothy KARPISHIN, Erika LEONARD, Shamali Roy CHOUDHURY
  • IMMUNOFLUORESCENCE ASSAYS
    Publication number: 20220057386
    Abstract: Provided herein are immunofluorescence assays that do not use an intensely colored lipophilic dye, and that result in a significant reduction of fluorescence due to aldehyde fixation, collagen, elastin, and/or red blood cells. In certain embodiments of the present invention, a two-step treatment of the tissue is contemplated that leads to the incorporation of reduced heteropoly acids or salts thereof (e.g., phosphomolybdate species) in the tissue. This reagent binds tightly to hydrophilic regions of the tissue and effectively quenches the fluorescence in those regions. In doing so, the overall signal-to-noise ratio for the immunofluorescence assay is increased.
    Type: Application
    Filed: April 5, 2021
    Publication date: February 24, 2022
    Inventors: Timothy B. KARPISHIN, Pamela JAMES, Erika LEONARD
  • Immunofluorescence assays
    Patent number: 10969384
    Abstract: Provided herein are immunofluorescence assays that do not use an intensely colored lipophilic dye, and that result in a significant reduction of fluorescence due to aldehyde fixation, collagen, elastin, and/or red blood cells. In certain embodiments of the present invention, a two-step treatment of the tissue is contemplated that leads to the incorporation of reduced heteropoly acids or salts thereof (e.g., phosphomolybdate species) in the tissue. This reagent binds tightly to hydrophilic regions of the tissue and effectively quenches the fluorescence in those regions. In doing so, the overall signal-to-noise ratio for the immunofluorescence assay is increased.
    Type: Grant
    Filed: May 3, 2018
    Date of Patent: April 6, 2021
    Assignee: VECTOR LABORATORIES, INC.
    Inventors: Timothy B. Karpishin, Pamela James, Erika Leonard