Abstract: Disclosed are methods and compositions for producing xylosylated steviol glycosides that include a xylose residue, or an oligosaccharide moiety including a xylose residue, attached to the 19 carbon of the steviol base. A glycosyltransferase having UDP-xylose:19-steviol xylosyltransferase activity (such as one of SEQ ID NO:1 or a homolog or variant thereof) forms the xylosylated steviol glycoside using a steviol glycoside acceptor having a glucose residue attached to the 19 carbon of the steviol base. Glycosyltransferase variants with increased activity are also described.

Abstract: In some embodiments, a method of distributing toy objects is provided. The method includes: providing a toy object and a task identifier, the task identifier identifying a task; receiving, via a website, task-completion input from a user; associating the user with a profile, the profile being associated with the toy object; updating the profile to indicate that the task had been performed; identifying reward criteria; determining whether the reward criteria was met; and, upon determining that the reward criteria was met, initializing distribution of a physical reward.