Abstract: This disclosure relates to methods and compositions for determining the alleles present at polymorphic simple sequence repeat (SSR) sites in the Cannabis genome to produce a genetic profile for Cannabis plants.

Abstract: Grafted cannabis plants and related methods for producing a grafted cannabis plant are provided. In some embodiments, a scion is provided from a first cannabis plant having at least one of a first desired phenotype and a first desired genotype and a rootstock is provided from a second cannabis plant having at least one of a second desired phenotype and a second desired genotype, the rootstock compatible with the scion. The scion may be grafted onto the rootstock to form the grafted plant. In some embodiments, the scion is from a drug-type cannabis plant and the rootstock is from a hemp plant. The grafted cannabis plant may have at least one enhanced plant trait, enhanced harvest trait, and/or emergent property. Related cannabis plant materials and cannabis-derived products are also provided.

Abstract: An economical and accurate method for identifying sex of a Cannabis plant involves amplifying an amplicon of genomic nucleic acid molecules from a subject Cannabis plant using polymerase chain reaction (PCR), the amplicon being a genomic region containing the nucleotide sequence as set forth in SEQ ID NO: 1. The sex of the subject Cannabis plant is determined by determining whether SEQ ID NO: 1 is SEQ ID NO: 2 or SEQ ID NO: 3, where SEQ ID NO: 2 is in male plants and SEQ ID NO: 1 is in female plants. Preferably, high resolution melt (HRM) analysis is used to determine the sex of the subject Cannabis plant by comparing the HRM analysis to a control for male or female Cannabis plants. The amplicon can be amplified in both male and female plants with a common primer set.