Abstract: A fluoroescence spectrometer or fluorometer for simultaneously presenting the excitation and emission spectra of a multicomponent sample in the form of an intensity matrix is disclosed which comprises an elongated cuvette for holding the fluoroescent sample, along whose length a spatially distributed spectrum of exciting energies is simultaneously directed, and a polychromatic analyzer which simultaneously analyzes the excitation and emission spectra given off by the sample along its length by producing a spectrally dispersed image of the cuvette. The matrix of spectral intensities so obtained may be converted to electrical signals by a low-light-level television detector and displayed on a video monitor or processed with appropriate analog or digital computers for immediate analysis. The instrument can acquire 241 emission spectra taken at 241 different exciting wavelengths in 16.7 milliseconds.