Abstract: Provided herein are microelectrodes and methods of localizing and targeting the same. The microelectrodes include electrochemical or biological sensors, an array of electrical contacts along a long axis of the microelectrode, or both. The methods of localizing and targeting use statistical manipulations to reduce the errors inherent in spike train analyses.

Abstract: A method for identifying neuronal spikes (extracellular action potentials) is described wherein measured microelectrode readings from tissue are reviewed to identify spikes (successive readings having prolonged rises and/or falls). The frequency of such spikes as a function of their amplitude assumes a bimodal distribution wherein higher amplitude spikes represent neuronal spikes (signal) and lower amplitude spikes represent noise, and thus the higher amplitude spikes can be assumed to be neuronal spikes. Neuronal spikes from the same neuron can then be assumed to have substantially the same waveform shape and period, with the only significant difference between them being the scaling of their amplitudes (i.e., the amplitudes of spikes from the same neuron tend to be proportionate at any given time along their period). Thus, by testing identified neuronal spikes for matching timing and for proportional amplitudes, the neuronal spikes may further be identified as coming from the same or different neurons.