Abstract: The invention features a purified embryonic or fetal caprine somatic cell. The somatic cell can be in a preparation of embryonic or fetal caprine somatic cells. In addition, the cell can be used to derive an embryonic or fetal caprine somatic cell line. The somatic cell can be a genetically engineered caprine somatic cell, e.g., a somatic cell which includes a transgenic sequence, or which a nucleic acid has been introduced.

Abstract: The present invention provides method to improve the efficiency of both serial nuclear transfer and the consequent production of transgenic cells and/or animals. This new method, through the use of at least a second round of nuclear transfer, will enable donor cells to correct their reprogramming more efficiently generating larger numbers of useful transgenic embryos. Because the reprogramming of the embryos generated is enhanced the current invention will allow for the production of healthier transgenic animals.